effect of intrauterine infusion of autologous platelet-rich plasma(PRP)on endometrial thickness and pregnancy rate as secondary endpoint in FET cycles.

Overal Objective: Evaluation of the effect of intrauterine infusion of platelet-rich plasma(PRP)on endometrial thickness in infertile women that have insufficient endometrial thickness in frozen embryo transfer cycles tients treated for frozen embryo transfer cycles after intrauterine infusion of PRP The endometrial thickness in patients treated for frozen embryo transfer cycles without intrauterine infusion of PRP Compare the endometrial thickness in patients treated for frozen embryo transfer cycles after intrauterine infusion of PRP and control group pregnancy rate in patients treated for frozen embryo transfer cycles after intrauterine infusion of PRP pregnancy rate in patients treated for frozen embryo transfer cycles without intrauterine infusion of PRP compare pregnancy rate in patients treated for frozen embryo transfer cycles after intrauterine infusion of PRP and group control Material and Method: In this study 100 infertile patients who have came to infertility clinic of mother and child hospital for transfer frozen embryo that have endometrial thickness less than 6 mm despite conventional treatment with low dose 6 mg estradiol in 10th day cycles.they randomized and divided to groups of 50 patients and after taking testimonial from study will start .excluding factors :platelet dysfunction syndrome ,thrombocytopenia ,continued use of the NSAIDS in the past 48 hours ,use corticosteroid during the past month,smoking,history of cancer ,recent fever ,plt<105000 In conventional treatment method Estradiol Valerate at 6 mg per day was given on day 2 of menstruation cycle and in all case to 8 mg increases and the maximum dose was 8 mg per day .similarly in first group the medication is continued and second group candidate for PRP. On the 10th day of HRT cycle 15 cc of venous blood was dramn from the syringe pre-filled with 5 cc of anticoagulant solution (ACD-A)and centrifuged immediately at 200 gr for 10 min .the blood was divided into three layers:red blood cells at the bottom ,cellular plasma in the supernatant and a buffy coat layer between them,the plasma layer and buffy coat were collected to another tube and re centrifuged 500 gr for 10 min.the resulting plellet of platelets was mixed with 1 cc of supernatant and then 0.5-1 cc of PRPwas obtained. It was infused into the uterus cavity immidiatly with Tomcat catheter .endometrial thickness was re-assessed 72 hour later .if the endometrial thickness was satisfied infusion of PRP was performed in 72 hr after first infusion. At last 2 group of case and control will be compared with each other by endometrial thickness before and after PRP infusion ,implantation rate ,pregnancy rate and number of canceling cycles.

Shiraz university of Medical Sciences