Objectives; Comparing the effect of repeated low dose and unique high dose sucrose with placebo (distilled water) on the control of pain secondary to retinopathy of prematurity examination of premature neonates. Design; Placebo controlled, double-blinded, randomized study. Setting and conduct; Single center, in Istanbul, Turkey. Participants including major eligibility criteria; Premature neonates who underwent first retinopathy of prematurity examination, who did not have asphyxia, intraventricular hemorrhage, who were not resuscitated, who had congenital anomaly, who underwent invasive or noninvasive mechanical ventilation therapy during retinopathy of prematurity examination, and who take analgesia or sedative medicine. Intervention; Distilled water group, three repeated doses of 0.2 ml sucrose (24%) and single dose of 0.6 ml sucrose (24%) group. Main outcome measures: Primary outcome measure was the “Premature Infant Pain Profile” (PIPP), which includes assessment of facial expressions, heart rate, oxygen saturation and took behavioral state and gestational age into consideration. Secondary outcome measures were heart rate differences, desaturation, crying time and the degree of cry.