For investigating the effectiveness of short-term vitamin E supplementation on lipid peroxidation at rest and response to exhaustive exercise Nineteen healthy male student with 66.9±6.5 Kg mean weight, 37/9±4 ml.Kg1min-1 maximal oxygen uptake, 21.9±2.5 body mass index and 21/1±1/2 mean age selected and randomly divided to supplement (n=9) and placebo group (n=10).Vitamin group consumed one capsule containing 400IU alpha tocopheryl asetat and placebo group 0.4 gr amylum in the same capsule during 45 minute before eating dinner for 14 days. Blood samples were taking before and after supplementation in two steps. Each step was done in two times before and immediately after exhaustive exercise on ergometer cycle following 12 to 14 hours with empty-stomached in a same condition. Serum malondialdehyde (index of lipidperoxidation) was estimated by method of thiobarbituric acid.
General information
Acronym
lipid peroxidation
IRCT registration information
IRCT registration number:IRCT2012081310578N3
Registration date:2012-12-23, 1391/10/03
Registration timing:retrospective
Last update:
Update count:0
Registration date
2012-12-23, 1391/10/03
Registrant information
Name
ٍEbrahim Moslehi
Name of organization / entity
Payamnoor University
Country
Iran (Islamic Republic of)
Phone
+98 74 1223 3595
Email address
ebrahim.moslehi@gmail.com
Recruitment status
Recruitment complete
Funding source
Kohgiloye & Boyerahmad Payamnoor Universiy
Expected recruitment start date
2012-04-20, 1391/02/01
Expected recruitment end date
2012-06-19, 1391/03/30
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
Effect of two week vitamin E supplementation on lipid peroxidation at rest and response to exhaustive exercise
Public title
Purpose
Basic scienece
Inclusion/Exclusion criteria
Inclusion criteria: being a student; male; healthy; non-athlete; living in student dormitory; following the students nutrition food menu; not doing regular exercise; born in native regions below 1000 meter height; not smoking; not using alcohol, caffeine, drugs and antioxidant food such as vegetables, fish oil, soya and any other antioxidant supplement which isn't included in research process; eating one capsule contained vitamin E or placebo 45 min before dinner for two week; doing exhaustive exercise in two stage before and after two week supplementation on ergo meter cycle; having permission to get 10 cc blood from brachial vein in two stage before and after supplementation immediately pre and post exhaustive exercise.
Age
From 21 years old to 22 years old
Gender
Male
Phase
N/A
Groups that have been masked
No information
Sample size
Target sample size:
19
Randomization (investigator's opinion)
Randomized
Randomization description
Blinding (investigator's opinion)
Double blinded
Blinding description
Placebo
Used
Assignment
Parallel
Other design features
Secondary Ids
empty
Ethics committees
1
Ethics committee
Name of ethics committee
Kohgiloye & Boyerahmad Payamnoor Universiy
Street address
Payamnoor University, Shahid Ghandi street, Yasuj
City
Yasuj
Postal code
Approval date
2012-04-20, 1391/02/01
Ethics committee reference number
2331/245/د
Health conditions studied
1
Description of health condition studied
ICD-10 code
ICD-10 code description
Primary outcomes
1
Description
lipid peroxidation (malondi aldehyde)
Timepoint
befor and after two week vitamin E consumption, pre and post exhostive exercise
Method of measurement
nanomol per litr
Secondary outcomes
1
Description
alteration of plasma vulome
Timepoint
befor and after suplementation response to exhustive exercise
Method of measurement
with Dill & Costill coefication by CBC Experiment
Intervention groups
1
Description
Two week vitamin E consumption 45 min before dinner
Category
Treatment - Drugs
2
Description
exhustve execise
Category
Other
Recruitment centers
1
Recruitment center
Name of recruitment center
Yasuj Payamnoor University
Full name of responsible person
Ebrahim Moslehi
Street address
Yasuj Payamnoor University
City
Yasuj
Sponsors / Funding sources
1
Sponsor
Name of organization / entity
Payamnoor University
Full name of responsible person
Ebrahim Moslehi
Street address
payamnoor university, Shahid Ghandi, Yasuj
City
Yasuj
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?