Protocol summary

Summary
This randomized double blind placebo controlled trial design with aim to determine the effect of chlorella vulgaris supplementation on oxidative stress and inflammatory indices on non-alcoholic fatty liver disease (NAFLD) patients. 70 patients with NAFLD will be randomly divided into 2 intervention and placebo groups. Subjects in two intervention and placebo group will receive 4 tablets of chlorella vulgaris daily (each containing 300 mg, daily 1200 mg) or 4 tablets of placebo respectively for two month. For all of the participants, personality questionnaire and 3 dietary records (0,4.8 weeks) will be filled for assessment of nutrient intake and food frequency questionnaire for food habit evaluation. The anthropometric and biochemical measurements at first and end of the study will be repeated.

General information

Acronym
IRCT registration information
IRCT registration number: IRCT2012110911220N1
Registration date: 2012-11-16, 1391/08/26
Registration timing: prospective

Last update:
Update count: 0
Registration date
2012-11-16, 1391/08/26
Registrant information
Name
Soodabeh Aliashrafi
Name of organization / entity
Student Research committee, Nutrition and Health faculty
Country
Iran (Islamic Republic of)
Phone
+98 41 1385 2090
Email address
aliashrafis@tbzmed.ac.ir
Recruitment status
Recruitment complete
Funding source
Student research committe, Health and Nutrition Faculty, Tabriz University of Medical Sciences
Expected recruitment start date
2012-11-21, 1391/09/01
Expected recruitment end date
2013-02-19, 1391/12/01
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
The Effect of Chlorella Vulgaris Supplementation on Metabolic, Blood Pressure and Antropometric Indices on Non-alcoholic fatty liver disease (NAFLD) patients
Public title
The Effect of Chlorella Vulgaris Supplementation on Metabolic, Blood Pressure and Antropometric Indices on Non-alcoholic fatty liver disease (NAFLD) patients
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria: Liver steatosis on sonographic image; 20-50 years old; BMI>=30; moderately activity. Exclusion criteria: Alcohol intake more than 20g/d; gestation or lactation or Menopause; very active or inpatients; Cardiovascular disease; Pulmonary disease; renal disease; liver transplantation; other liver diseases and chronic or acute liver disease(Hepatitis A,B, ...); bilious impairments; cancers; inheritance diseases; using drugs like, insulin sensitivity enhancer, hepatotoxic drugs and estrogen
Age
From 20 years old to 50 years old
Gender
Both
Phase
N/A
Groups that have been masked
No information
Sample size
Target sample size: 50
Randomization (investigator's opinion)
Randomized
Randomization description
Blinding (investigator's opinion)
Double blinded
Blinding description
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
The Research Ethics Committee of Tabriz University of Medical Sciences
Street address
Tabriz University of Medical Sciences, Attar Neyshabori Street
City
Tabriz
Postal code
Approval date
2012-03-12, 1390/12/22
Ethics committee reference number
9129

Health conditions studied

1

Description of health condition studied
Non-alcoholic fatty liver disease
ICD-10 code
K76.0
ICD-10 code description
Fatty (change of) liver, not elsewhere classified

Primary outcomes

1

Description
Liver Enzyme (ALT,AST,ALP)
Timepoint
Beginning and end of the intervention
Method of measurement
IFCC

2

Description
Liver steatosis
Timepoint
Begining and end of intervention
Method of measurement
Liver Sonography

3

Description
FBS
Timepoint
Begining and end of intervention
Method of measurement
Enzymatic method

4

Description
TC, TG, LDL-C, HDL_c
Timepoint
Begining and end of intervention
Method of measurement
Enzymatic method forTC,TG and HDL-c for LDL-c:Freid wals formula

5

Description
Blood pressure
Timepoint
Begining and end of intervention
Method of measurement
Mercury manometer

6

Description
anthropometry
Timepoint
Begining and end of intervention
Method of measurement
Scale and meter

Secondary outcomes

1

Description
Energy and nutrient intake
Timepoint
beginning and end of the intervention
Method of measurement
Dietary record and food frequency questionnaire

Intervention groups

1

Description
Intervention Group: 300 mg chlorella vulgaris, 4 tablets/day for 8 weeks
Category
Treatment - Drugs

2

Description
Placebo groups: placebo, 4 tablets/day for 8 weeks
Category
Placebo

Recruitment centers

1

Recruitment center
Name of recruitment center
Sheykholrais clinic
Full name of responsible person
Street address
City
Tabriz

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Tabriz University of Medical Sciences
Full name of responsible person
Dr. Ostadrahimi
Street address
Tabriz University of Medical Sciences, Attar Neyshabori Street, Golgasht Street
City
Tabriz
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Tabriz University of Medical Sciences
Proportion provided by this source
100
Public or private sector
empty
Domestic or foreign origin
empty
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
empty

Person responsible for general inquiries

Contact
Name of organization / entity
Student Serearch Committee, Nutrition and Health faculty,Tabriz university of medical sciences
Full name of responsible person
Soodabeh Aliashrafi
Position
Master Science Student of Nutrition
Other areas of specialty/work
Street address
Tabriz University of Medical Sciences, Attar Neyshabori Street, Golgasht Street
City
Tabriz
Postal code
Phone
+98 41 1334 1113
Fax
Email
Aliashrafis@tbzmed.ac.ir ; SA.NUY89@yahoo.com ; SAliashrafi@gmail.com
Web page address

Person responsible for scientific inquiries

Contact
Name of organization / entity
Student research committe, Health and nutrition faculty, Tabriz University of Medical Sciences
Full name of responsible person
Soodabeh Aliashrafi
Position
Master Science Student of Nutrition
Other areas of specialty/work
Street address
Health and Nutrition Faculty,Tabriz University of Medical Sciences, Attar Neyshabori Street, Golgasht Street
City
Tabriz
Postal code
Phone
+98 41 1334 1113
Fax
Email
Aliashrafis@tbzmed.ac.ir ; SA.NUT89@yahoo.com ; SAliashrafi@gmail.com
Web page address

Person responsible for updating data

Contact
Name of organization / entity
Student research committee, Tabriz University of Medical Sciences, Health and nutrition faculty
Full name of responsible person
Soodabeh Aliashrafi
Position
Master Science Student of Nutrition
Other areas of specialty/work
Street address
Health and Nutrition Faculty,Tabriz University of Medical Sciences, Attar Neyshabori Street, Golgasht Street
City
Tabriz
Postal code
Phone
+98 41 1336 6581
Fax
Email
Aliashrafis@tbzmed.ac.ir ; SA.NUT89@yahoo.com ; SAliashrafi@gmail.com
Web page address

Sharing plan

Deidentified Individual Participant Data Set (IPD)
empty
Study Protocol
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Statistical Analysis Plan
empty
Informed Consent Form
empty
Clinical Study Report
empty
Analytic Code
empty
Data Dictionary
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