Aim: This study will conduct to determine the effect of resistance exercise with different rest intervals between sets on the apoptosis biomarkers in athletes. Methods: Fifteen trained male (aged 18-29 years) will perform two experimental sessions that consisted of resistance exercise in six sessions resistance exercise performed for four sets with 85% 1RM load.
On experimental day 1, the subjects performed all exercises with the 1-minute rest between sets, then they did all the exercises with the 3-minute rest on test day 2 (at least 72 hours later). Blood samples will obtain pre-exercise, immediately post-exercise, 3 and 24 hours post-exercise for the measurement of the serum protein-p53, Caspase-3, Caspase-9, Insulin-like growth factor 1(IGF-1), Creatine kinase and plasma Lactate concentrations.
General information
Acronym
ApopTra2013
IRCT registration information
IRCT registration number:IRCT2013020612386N1
Registration date:2013-03-17, 1391/12/27
Registration timing:prospective
Last update:
Update count:0
Registration date
2013-03-17, 1391/12/27
Registrant information
Name
Hassan Faraji
Name of organization / entity
Islamic Azad University, Mariwan Branch
Country
Iran (Islamic Republic of)
Phone
+98 87 5330 3954
Email address
farajienator@gmail.com
Recruitment status
Recruitment complete
Funding source
The research will funded by Islamic Azad University, Mariwan Branch.
Expected recruitment start date
2013-04-08, 1392/01/19
Expected recruitment end date
2014-02-07, 1392/11/18
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
The effect of resistance exercise with different rest intervals between sets on the apoptosis biomarkers in athletes
Public title
Effect of exercise on apoptosis biomarkers
Purpose
Basic scienece
Inclusion/Exclusion criteria
Inclusion criteria: males; athletes; aged 18-29 years; healthy; without consume any anti-inflammatory or oxidative supplementation (during 6 months prior to the study).
Exclusion criteria: smoking; chronic diseases such as diabetes, cardiovascular disease, atherosclerosis and hypertension; muscle injures; uncontrolled intake of oxidative and anti-inflammatory drags supplements and stimulants (during the period).
Age
From 18 years old to 29 years old
Gender
Male
Phase
N/A
Groups that have been masked
No information
Sample size
Target sample size:
15
Randomization (investigator's opinion)
N/A
Randomization description
Blinding (investigator's opinion)
Not blinded
Blinding description
Placebo
Not used
Assignment
Single
Other design features
Secondary Ids
empty
Ethics committees
1
Ethics committee
Name of ethics committee
Tabriz University of Medical Sciences
Street address
Golgasht St. Daneshgah St. Tabriz, East Azarbaijan, Iran. Islamic republic of Iran
City
Tabriz
Postal code
Approval date
2013-01-07, 1391/10/18
Ethics committee reference number
91171
Health conditions studied
1
Description of health condition studied
Encounter exercise for healthy administratin
ICD-10 code
Z02
ICD-10 code description
Examination and encounter for administrative purposes
Primary outcomes
1
Description
Serum protein-p53
Timepoint
Pre-exercise, immediately post-exercise, 3 and 24 hours post-exercise
Method of measurement
ELISA
2
Description
Caspase-3
Timepoint
Pre-exercise, immediately post-exercise, 3 and 24 hours post-exercise
Method of measurement
ELISA
3
Description
Caspase-9
Timepoint
Pre-exercise, immediately post-exercise, 3 and 24 hours post-exercise
Method of measurement
ELISA
4
Description
Insulin-like growth factor 1(IGF-1)
Timepoint
Pre-exercise, immediately post-exercise, 3 and 24 hours post-exercise
Method of measurement
ELISA
Secondary outcomes
1
Description
Creatine Kinase
Timepoint
Pre-exercise, immediately post-exercise, 3 and 24 hours post-exercise
Method of measurement
Enzymatic
2
Description
Plasma Lactate
Timepoint
Pre-exercise, immediately post-exercise, 3 and 24 hours post-exercise
Method of measurement
Enzymatic
Intervention groups
1
Description
In the1th exercise session, rest between sets will perform 1 minute and in the 2th exercise session rest between sets well perform 3 minutes.
Category
Other
Recruitment centers
1
Recruitment center
Name of recruitment center
Islamic Azad University, Mariwan Branch
Full name of responsible person
Hassan Faraji
Street address
Department of physical education and sport sciences, Islamic Azad University, Mariwan, Kourdestan, Iran
City
Mariwan
Sponsors / Funding sources
1
Sponsor
Name of organization / entity
Islamic Azad University, Mariwan Branch
Full name of responsible person
Abdollah Shaeisteh
Street address
Department of physical education and sport sciences, Islamic Azad University, Mariwan, Kourdestan , Iran
City
Mariwan
Grant name
32291
Grant code / Reference number
7892
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Islamic Azad University, Mariwan Branch
Proportion provided by this source
100
Public or private sector
empty
Domestic or foreign origin
empty
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
empty
Person responsible for general inquiries
Contact
Name of organization / entity
Kurdestan University
Full name of responsible person
Dr. Rahman Rahimi
Position
PhD/assistance professor of exercise physiology
Other areas of specialty/work
Street address
Department of physical education and sport sciences, Kurdestan University, Kurdestan,Sanandaj, Iran
City
Sanandaj
Postal code
6617715175
Phone
+98 87 1666 4600
Fax
Email
Rahman.rahimi@yahoo.com
Web page address
Person responsible for scientific inquiries
Contact
Name of organization / entity
Islamic Azad University, Mariwan Branch
Full name of responsible person
Hassan Faraji
Position
PhD student of exercise physiology
Other areas of specialty/work
Street address
Department of physical education and sport sciences, Islamic Azad University, Mariwan, Kourdestan , Iran
City
Mariwan
Postal code
Phone
+98 87 5322 0186
Fax
Email
farajienator@gmail.com
Web page address
Person responsible for updating data
Contact
Name of organization / entity
Islamic Azad University, Mariwan Branch
Full name of responsible person
Hasan Faraji
Position
PhD student of exercise physiology
Other areas of specialty/work
Street address
Department of physical education and sport sciences, Islamic Azad University, Mariwan, Kourdestan , Iran
City
Mariwan
Postal code
Phone
+98 87 5332 0186
Fax
+98 87 5322 0186
Email
farajienator@gmail.com
Web page address
Sharing plan
Deidentified Individual Participant Data Set (IPD)