Protocol summary

Summary
Traumatic brain injury is an important public health problem and a leading cause of injury -related death and disability. Inflammatory and anti- apoptotic effects and significantly reduces secondary damage. Although pre-clinical studies show the advantage of progesterone, and there are positive results two human clinical trials, more clinical trials are needed to confirm the use of progesterone in acute traumatic brain injury. Taken the neuroprotective potential benefits of estrogen therapy, time is for controlled clinical trials of estrogen therapy in traumatic brain injury. Therefore, in this study, the probable efficacy and potential mechanism of estrogen and progesterone in reduction of brain damage in patients with moderate and severe traumatic brain injury, 18 to 60 years old admitted in Kerman Shahid Bahonar Hospital, in a Randomized, double-blind, placebo- controlled clinical trial are tested . In this study inclusion criteria: diffuse TBI; being male; having age between 18 to 60 years of age and severity of moderate to severe brain injury with GCS (Glasgow Coma Scale) (with grades 3-8 as severe and 9-12 as moderate). Exclusion criteria: patients with blunt head trauma; damage with unknown time; entering the hospital with damage time more than 4 hours; severe hypothermia (temperature less than 28 ° C); age less than 18 years and more than 60 years; SCI, patients who are candidates for craniotomy; diseases related to the immune system, gastrointestinal, oncology, infectious diseases, and other associated trauma are. Patients are divided randomly 3 groups; Control group: in this group, only routine and standard treatment is done. Progesterone group: in addition to routine and standard treatment, progesterone dose 1 mg /kg within 4 hours of brain damage is administered intramuscularly, and then this dose is repeated every 12 hours for 5 consecutive days. Estrogen group: in addition to routine and standard treatment, estrogen dose 1/25 mg within 4 hours of brain damage is administered oral, and then this dose is repeated everyday for 5 consecutive days. In this study, GOS (Glasgow-Outcome Scale-Extended), FIM (Functional Independence Measure), and CRS-R (coma recovery scale – revised) are measured after resuscitation, time discharge, 3 and 6 months after TBI. Serum inflammatory factors, Serum brain proteins, Serum MDA (Malone dialdeid ) Levels, Serum protein carbonyl content, and Serum levels of total antioxidant activity are measured, After resuscitation, 24 hours and 6 days after TBI.

General information

Acronym
IRCT registration information
IRCT registration number: IRCT2014042017356N1
Registration date: 2014-08-21, 1393/05/30
Registration timing: registered_while_recruiting

Last update:
Update count: 0
Registration date
2014-08-21, 1393/05/30
Registrant information
Name
Zahra Soltani
Name of organization / entity
Kerman University of Medical Sciences
Country
Iran (Islamic Republic of)
Phone
+98 34 1322 0081
Email address
z.soltani@kmu.ac.ir
Recruitment status
Recruitment complete
Funding source
Kerman Neuroscience Research Center, Research Council of Kerman University of Medical Sciences
Expected recruitment start date
2014-05-22, 1393/03/01
Expected recruitment end date
2016-05-21, 1395/03/01
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
Evaluation of the efficacy and probable mechanism of estrogen and progesteron on the complications of moderate and severe diffuse traumatic brain injury in patients admitted in Kerman Shahid Bahonar Hospital: a clinical trial
Public title
Effect of female sex hormones on traumatic brain injury
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria: diffuse TBI; being male; having age between 18 to 60 years of age and severity of moderate to severe brain injury with GCS (Glasgow Coma Scale) (with grades 3-8 as severe and 9-12 as moderate). Exclusion criteria: patients with blunt head trauma; damage with unknown time; entering the hospital with damage time more than 4 hours; severe hypothermia (temperature less than 28 ° C); age less than 18 years and more than 60 years; SCI, patients who are candidates for craniotomy; diseases related to the immune system, gastrointestinal, oncology, infectious diseases, and other associated trauma.
Age
From 18 years old to 60 years old
Gender
Male
Phase
N/A
Groups that have been masked
No information
Sample size
Target sample size: 90
Randomization (investigator's opinion)
Randomized
Randomization description
Blinding (investigator's opinion)
Double blinded
Blinding description
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Research Council of Kerman University of Medical Sciences
Street address
Sina Avenue, Jahad blvd., somayeh cross
City
Kerman
Postal code
Approval date
2014-04-13, 1393/01/24
Ethics committee reference number
92/579/کا

Health conditions studied

1

Description of health condition studied
Traumatic brain injury
ICD-10 code
S06.2
ICD-10 code description
Diffuse brain injury

Primary outcomes

1

Description
GOS (Glasgow-Outcome Scale-Extended )
Timepoint
after resuscitation, time discharge, 3 and 6 months after TBI
Method of measurement
Questionnaire

2

Description
FIM (Functional Independence Measure )
Timepoint
after resuscitation, time discharge, 3 and 6 months after TBI
Method of measurement
Questionnaire

3

Description
CRS-R ( coma recovery scale – revised )
Timepoint
After resuscitation, time discharge, 3 and 6 months after TBI
Method of measurement
Questionnaire

4

Description
serum infammatory factors
Timepoint
After resuscitation, 24 hours and 6 days after TBI
Method of measurement
ELISA

5

Description
Serum brain proteins
Timepoint
After resuscitation, 24 hours and 6 days after TBI
Method of measurement
ELISA

6

Description
Serum MDA(Malon dialdeid ) Levels
Timepoint
After resuscitation, 24 hours and 6 days after TBI
Method of measurement
Thiobarbituric acid method

7

Description
Serum protein carbonyl content
Timepoint
After resuscitation, 24 hours and 6 days after TBI
Method of measurement
DNPH(2, 4-D Nytrvfnl hydrazine) method

8

Description
Serum levels of total antioxidant activity
Timepoint
After resuscitation, 24 hours and 6 days after TBI
Method of measurement
ferric reducing of antioxidants power (FRAP)

Secondary outcomes

1

Description
mortality
Timepoint
24 hours, and 1, 3 and 6 months after TBI
Method of measurement
number

2

Description
complications and adverse effects
Timepoint
24 hours, and 1, 3 and 6 months after TBI
Method of measurement
visual

3

Description
Length of stay
Timepoint
Hospitalization time
Method of measurement
visual

4

Description
differenace GCS between Admission and discharge times
Timepoint
hospitalization
Method of measurement
visual

Intervention groups

1

Description
Control group: in this group, only routine and standard treatment is done.
Category
Placebo

2

Description
Progesterone group: in addition to routine and standard treatment, progesterone dose 1 mg /kg within 4 hours of brain damage is administered intramuscularly, and then this dose is repeated every 12 hours for 5 consecutive days.
Category
Treatment - Drugs

3

Description
Estrogen group: in addition to routine and standard treatment, estrogen dose 1/25 mg within 4 hours of brain damage is administered oral, and then this dose is repeated everyday for 5 consecutive days.
Category
Treatment - Drugs

Recruitment centers

1

Recruitment center
Name of recruitment center
Kerman Shahid Bahonar Hospital
Full name of responsible person
Saeed Karamouzian
Street address
City
Kerman

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Kerman Neuroscience Research Center
Full name of responsible person
Dr. Vahid Sheibani
Street address
Sina Avenue, Jahad blvd., somayeh cross
City
Kerman
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Kerman Neuroscience Research Center
Proportion provided by this source
Public or private sector
empty
Domestic or foreign origin
empty
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
empty

2

Sponsor
Name of organization / entity
Research Council of Kerman University of Medical Sciences
Full name of responsible person
Dr. Abbass Pardakhti
Street address
Sina Avenue, Jahad Blvd.,somayeh cross
City
Kerman
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Research Council of Kerman University of Medical Sciences
Proportion provided by this source
Public or private sector
empty
Domestic or foreign origin
empty
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
empty

Person responsible for general inquiries

Contact
Name of organization / entity
Kerman University of Medical Sciences
Full name of responsible person
Dr. Mohammad Khaksari
Position
Professor
Other areas of specialty/work
Street address
Department of Physiology, Afzalipour Medicine College,22 bahman Blvd.
City
Kerman
Postal code
Phone
+98 34 1322 0081
Fax
Email
physiolojy@kmu.ac.ir
Web page address

Person responsible for scientific inquiries

Contact
Name of organization / entity
Kerman University of Medical Sciences
Full name of responsible person
Dr. Mohammad Khaksari
Position
Professor
Other areas of specialty/work
Street address
Department of Physiology, Afzalipour Medicine College,22 bahman Blvd.
City
Kerman
Postal code
Phone
+98 34 1322 0081
Fax
Email
physiolojy@kmu.ac.ir
Web page address

Person responsible for updating data

Contact
Name of organization / entity
Kerman University of Medical Sciences
Full name of responsible person
Zahra Soltani
Position
Ph.D. Student of Physiology
Other areas of specialty/work
Street address
Department of Physiology, Afzalipour Medicine College,22 bahman Blvd.
City
Kerman
Postal code
Phone
+98 34 1322 0081
Fax
Email
Web page address

Sharing plan

Deidentified Individual Participant Data Set (IPD)
empty
Study Protocol
empty
Statistical Analysis Plan
empty
Informed Consent Form
empty
Clinical Study Report
empty
Analytic Code
empty
Data Dictionary
empty
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