There are many researches on relationship between stress and depression with migraine; however, much experimental evidence is needed to make such a view more validated. The aim of this study was experimental investigation of mood induction and stress on pain intensity in migraine patients. Patients were included in the study if they were: aged between 18 and 65, had a formal diagnosis of migraine by a neurologist, had a complaint of migraine for six months or longer and had a high school certificate (12 years of formal education or higher). Patients excluded from the study were those with severe depression or anxiety, severe daily headache and pregnancy. The study population was all patients suffering migraine in Tehran. For this purpose, 60 migraine patients were recruited from Baqiyatallah Hospital in Tehran-Iran in 2011-2012 years, and were randomly assigned into one of three groups: negative mood induced group, positive mood induced group and control group. The following instruments and measures were used in this study: Mood induction by presenting different types of films, and a computerized cognitive task to elicit pain. The pain intensity was measured in three steps (at baseline, after computerized task, and after showing film). All eligible participants were asked to complete baseline self-report measure of pain intensity prior to receiving pain and mood induction. Following this, computerized task of cognitive exhaustion to elicit pain was used, and after this stage, the mood induction procedure by showing a film was applied.