The purpose of the study is to examine the effect of multisensory stimulation on the pain caused by eye examination in an attempt to administer screening examinations for retinopathy of prematurity. In this randomized clinical trial, 80 preterm infants with a gestational age of 32 weeks or less based on baby’s records, and infants who weigh less than 1500 gr at birth, or infants who weigh 1500 to 2000 gr and suffer from a severe systematic disease and age 4 weeks, were selected by an available sampling, and randomly divided into two groups; intervention and control. In the intervention group, a multisensory stimulation program runs. For tactile stimulation, baby’s face, upper and lower limbs will be gently caressed by her mother. For visual stimulation, mother takes a close look at baby’s face and attempts to make eye contact with her. For auditory stimulation, mother gently and continuously talks to her baby. For olfactory stimulation, the smell of vanilla will be used; which runs 15 minutes before examination starts and lasts until the examination starts. For gustatory stimulation, 33% glucose was used 2 minutes before examination starts. The control group received ordinary care in the ward. PIPP was used for pain assessment tool, the pain was examined in eight stages before intervention, before examination starts, during first eye and second eye examination, and in 4 30-second stages immediately after the end of eye examination.