Protocol summary

Study aim
Evaluating the effect of increasing the amount of milk protein content by protein supplement on growth indices of preterm infants
Design
A concealed, randomized, blinded, clinical trial with a parallel group design of 30 patients, enrolled between March 2014 and March 2015. ّ
Settings and conduct
The study was conducted at Imam Khomeini Hospital's NICU in Tehran. Newborns were randomly divided into two groups. By enteral feeding beginning we have added protein supplement to reach 4 grams per kilogram per day as total protein receiving per day which compare to the Control group who has taken 3.5 grams per kilogram per day protein with their enteral feeding. Its effects on neonatal growth indices, days of hospitalization, full fed time were recorded. Double-blinded study, which the patients, the nurses, the conductors, and the analyst _who later joined the research team and was not a member initially _ were not aware.
Participants/Inclusion and exclusion criteria
Inclusion criteria: Gestational age less than 34 weeks; Birth weight less than 1500 grams. Exclusion criteria: Growth affecting anomalies; Parents disapproval; Unable to continue feeding for more than 72 hours; Urea over than 60 milligram per deciliter
Intervention groups
Participant in intervention group are premature infants born < 32 complete week's gestational and birthweight les than 1500 gram who will receive high protein diet, reaching the goal of 4 grams per kilogram per day. Participant in intervention group are also premature infants born < 32 complete week's gestational and birthweight les than 1500 gram who will receive standard protein diet which is 3.5 grams per kilogram per day. All participants' growth indices, days of hospitalization and full fed time were recorded.
Main outcome variables
Growth indices; Length of hospitalization; Oral tolerance

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT20141118019991N3
Registration date: 2021-09-16, 1400/06/25
Registration timing: retrospective

Last update: 2021-09-16, 1400/06/25
Update count: 0
Registration date
2021-09-16, 1400/06/25
Registrant information
Name
Saharnaz Talebiyan
Name of organization / entity
Tehran university of medical science
Country
Iran (Islamic Republic of)
Phone
+98 21 6119 2407
Email address
s_talebiyan@yahoo.com
Recruitment status
Recruitment complete
Funding source
TEHRAN UNIVERSITY OF MEDICAL SCIENCE
Expected recruitment start date
2014-03-20, 1392/12/29
Expected recruitment end date
2015-03-20, 1393/12/29
Actual recruitment start date
2014-03-20, 1392/12/29
Actual recruitment end date
2015-03-20, 1393/12/29
Trial completion date
2015-03-20, 1393/12/29
Scientific title
Evaluating the effect of increasing the amount of milk protein content by protein supplement on growth indices of preterm infants
Public title
Effect of increasing the amount of milk protein content by protein supplement on growth indices of preterm infants
Purpose
Supportive
Inclusion/Exclusion criteria
Inclusion criteria:
Gestational age less than 34 weeks Birth weight less than 1500 grams No anomalies which affect on growth
Exclusion criteria:
Parents disapproval Unable to continue feeding more than 72 hours Urea over than 50 milligram per deciliter
Age
From 1 day old to 30 days old
Gender
Both
Phase
N/A
Groups that have been masked
  • Participant
  • Care provider
  • Outcome assessor
  • Data analyser
Sample size
Target sample size: 30
Actual sample size reached: 30
Randomization (investigator's opinion)
Randomized
Randomization description
We will Use permuted block randomization and assign participants 1:1 to the standard of care diet (control) or higher protein Diet (intervention). Twins will be randomized individually. The study biostatistician will generate the allocation sequence and place the research assignments in envelopes, to be opened by research assistants on the study start date.
Blinding (investigator's opinion)
Double blinded
Blinding description
Parents, clinical staff, and all study staff apart from the research assistants who prepare the study diet will be blinded to the study group. Unblinding will be permitted if an error in milk preparation is identified, or if unexpected safety concerns arise that require unblinding.
Placebo
Not used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Tehran University of Medical Sciences
Street address
Imam Khomeini Hospital Complex, Tohid Squre, Tehran, Iran
City
Tehran
Province
Tehran
Postal code
1419733141
Approval date
2016-01-30, 1394/11/10
Ethics committee reference number
IR.TUMS.REC.1394.1781

Health conditions studied

1

Description of health condition studied
Very Low Birth Weight
ICD-10 code
P07.00
ICD-10 code description
Extremely low birth weight newborn, unspecified weight

Primary outcomes

1

Description
Weight
Timepoint
Daily from the first day of life
Method of measurement
Digital scale

2

Description
Height
Timepoint
Weekly from the first day of life
Method of measurement
meter

3

Description
Head Circumference
Timepoint
Weekly from the first day of life
Method of measurement
meter

Secondary outcomes

1

Description
Urea
Timepoint
Biweekly
Method of measurement
Blood Sample Enzymatic tests (gr/dl)

2

Description
‌Bicarbonate
Timepoint
Biweekly
Method of measurement
Blood Sample enzymatic auto-analyzer

3

Description
Calcium
Timepoint
Biweekly
Method of measurement
Blood Sample Enzymatic tests (mg/dl)

4

Description
Phosphorus
Timepoint
Biweekly
Method of measurement
Blood Sample Enzymatic tests (mg/dl)

5

Description
Alkaline phosphatase
Timepoint
Biweekly
Method of measurement
Blood Sample Enzymatic tests

6

Description
Albumin
Timepoint
Biweekly
Method of measurement
Blood Sample Enzymatic tests

7

Description
Total protein
Timepoint
Biweekly
Method of measurement
Blood Sample Enzymatic tests (gr/dl)

8

Description
Clinical and Para-clinical sign of Necrotizing Enterocolitis
Timepoint
Multiple clinical Evaluation per day, and if needed also Para-clinical evaluation
Method of measurement
Clinical and Para-clinical Signs and symptoms.

9

Description
Sepsis Early or Late Onset, clinical or Para-clinical Sign and Symptoms.
Timepoint
Multiple clinical Evaluation per day, and if needed also Para-clinical evaluation
Method of measurement
Clinical and Para-clinical Signs and Symptoms

10

Description
Hospitalization Duration
Timepoint
Hospitalization Duration by days number at discharge.
Method of measurement
Hospitalization Duration days

Intervention groups

1

Description
Intervention group: High protein diet (4 grams per kilogram per day)  which is provided by protein supplement, when the enteral feeding has been started.
Category
Treatment - Other

2

Description
Control group: Routine protein diet (3.5 grams per kilogram per day)  which is provided by protein supplement, when the enteral feeding has been started.
Category
Treatment - Other

Recruitment centers

1

Recruitment center
Name of recruitment center
Breastfeeding research center Tehran university of medical sciences
Full name of responsible person
Saharnaz Talebiyan
Street address
Imam Khomeini Hospital Complex, Tohid Squre, Tehran, Iran
City
Tehran
Province
Tehran
Postal code
1419733141
Phone
+98 21 2274 9212
Email
s_talebiyan@yahoo.com

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Tehran University of Medical Sciences
Full name of responsible person
Vice-Chancellor in Research Affairs
Street address
6th floor, Central University Organization, Qods Street, Keshavarz Boulevard
City
Tehran
Province
Tehran
Postal code
1419733141
Phone
+98 21 8163 3698
Email
vcr@tums.ac.ir
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Tehran University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Tehran University of Medical Sciences
Full name of responsible person
Saharnaz Talebiyan
Position
Consultant
Latest degree
Subspecialist
Other areas of specialty/work
Pediatrics
Street address
Vali-asr hospital, Imam Khomeini Hospital Complex, Tohid Squre
City
Tehran
Province
Tehran
Postal code
1419733141
Phone
+98 21 2274 9212
Fax
Email
s_talebiyan@yahoo.com
Web page address

Person responsible for scientific inquiries

Contact
Name of organization / entity
Tehran University of Medical Sciences
Full name of responsible person
Saharnaz Talebiyan
Position
Consultant
Latest degree
Subspecialist
Other areas of specialty/work
Pediatrics
Street address
Vali-asr hospital, Imam Khomeini Hospital Complex, Tohid Squre
City
Tehran
Province
Tehran
Postal code
1419733141
Phone
+98 21 2274 9212
Fax
Email
s_talebiyan@yahoo.com
Web page address

Person responsible for updating data

Contact
Name of organization / entity
Tehran University of Medical Sciences
Full name of responsible person
Saharnaz Talebiyan
Position
Consultant
Latest degree
Subspecialist
Other areas of specialty/work
Pediatrics
Street address
Vali-asr hospital, Imam Khomeini Hospital Complex, Tohid Squre
City
Tehran
Province
Tehran
Postal code
1419733141
Phone
+98 21 2274 9212
Fax
Email
s_talebiyan@yahoo.com
Web page address

Sharing plan

Deidentified Individual Participant Data Set (IPD)
No - There is not a plan to make this available
Justification/reason for indecision/not sharing IPD
No more information.
Study Protocol
Undecided - It is not yet known if there will be a plan to make this available
Statistical Analysis Plan
Undecided - It is not yet known if there will be a plan to make this available
Informed Consent Form
No - There is not a plan to make this available
Clinical Study Report
No - There is not a plan to make this available
Analytic Code
No - There is not a plan to make this available
Data Dictionary
Not applicable
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