This clinical trial was carried out to assess the effects of probiotics in the treatment of infantile colic in 84 breast fed and formula fed infants that have age less than 13 weeks who referred to Arak AmirKabir hospital Based on inclusion criteria: infantile colic (crying and restlessness more than 3 hours a day for more than 3 days a week); age less than 3 months, more than 36 weeks gestational age at birth, birth weight greater than 2500 g and Exclusion criteria: growth retardation, medical problems( immune compromise, genetic disorders) or taking solids, antibiotics or kidilact. Children were Randomly assigned in two groups, case groups: (treated with 5 drops once daily dose of probiotic Pedilact and voice and vocal technique recommended to reduce colic include: rhythmic rocking motion, walking, mild tremor, etc.) and the control group (only recommend techniques for reducing colic), respectively. Clinical improvement of infants was assessed within 28 days after start of intervention. Outcome variables were crying attacks, restlessness, and crying time in infants within 24 hours.