The effect of garlic supplementation on response to inflammatory and enzymatic indices of oxidative stress after an intense aerobic and resistance exercise in young inactive females
The effect of garlic supplementation on response to inflammatory and enzymatic indices of oxidative stress after an intense aerobic and resistance exercise in young inactive females
Design
Randomized clinical trail with with 40 volunteers in 4 groups. With placebo group, with parallel groups.
Settings and conduct
The present research is a clinical trail study on sedentary young girl. Aerobic exercise were done by Bruce protocol test and resistance exercises were done by bodybuilding machine as exhausting of 70, 75 and 80%.
For receive supplements and placebo, cans of two different colors was used in a bag (without the knowledge of the researcher and the subject). Exercise protocols and blood sampling were carried out at the club and heart clinic.
Participants/Inclusion and exclusion criteria
Inclusion criteria: Inactive girl with 18 to 30 years old, Having complete physical, Not have regular exercise training during previous 3 months
Exclusion criteria: Having allergy to garlic, Blood coagulation disease
Intervention groups
Intervention group 1: Aerobic exercise including Bruce protocol test combine with consumption of garlic tablet 500 mg , twice a day and for 2 weeks. Intervention group 2: Aerobic exercise including Bruce protocol test on treadmill combine with consumption of lactose capsule 500 mg , twice a day for 2 weeks.Intervention group 3: Resistance exercises were done by bodybuilding machine as exhausting of 70, 75 and 80% and the rest time for each station was 30 seconds and between each set was 3 minutes combine Similar to garlic supplementation. Intervention group 4: Resistance exercises were done by bodybuilding machine as exhausting of 70, 75 and 80% and the rest time for each station was 30 seconds and between each set was 3 minutes combine with consumption of lactose capsule 500 mg , twice a day for 2 weeks.
Main outcome variables
inflammatory and enzymatic indices
General information
Reason for update
Acronym
IRCT registration information
IRCT registration number:IRCT20151210025466N2
Registration date:2019-01-29, 1397/11/09
Registration timing:retrospective
Last update:2019-01-29, 1397/11/09
Update count:0
Registration date
2019-01-29, 1397/11/09
Registrant information
Name
Shahram Gholamrezaei Darsara
Name of organization / entity
Azad University
Country
Iran (Islamic Republic of)
Phone
+98 13 3342 3308
Email address
gholamrezaei@iaurasht.ac.ir
Recruitment status
Recruitment complete
Funding source
All costs were funded solely by researcher
Expected recruitment start date
2015-07-01, 1394/04/10
Expected recruitment end date
2015-10-02, 1394/07/10
Actual recruitment start date
2015-08-06, 1394/05/15
Actual recruitment end date
2015-08-23, 1394/06/01
Trial completion date
2015-08-23, 1394/06/01
Scientific title
The effect of garlic supplementation on response to inflammatory and enzymatic indices of oxidative stress after an intense aerobic and resistance exercise in young inactive females
Public title
The effect of garlic supplementation on blood indices
Purpose
Prevention
Inclusion/Exclusion criteria
Inclusion criteria:
Not have history of certain diseases
Having complete health based on the results of medical examinations
Lack of a history of diseases by the consumption of garlic
not consumption of herbal or drug supplements and Anti-inflammatory drugs or steroids during previous 3 months
Not have regular exercise training during previous 3 months
Out of 60 volunteers, 40 eligible subjects were randomly assigned ( Random number table ) to four groups:
Aerobic exercise, garlic supplement
Aerobic exercise, lactose placebo
Resistance exercise, garlic supplement
Resistance exercise, lactose placebo
Blinding (investigator's opinion)
Not blinded
Blinding description
Placebo
Used
Assignment
Parallel
Other design features
Secondary Ids
empty
Ethics committees
1
Ethics committee
Name of ethics committee
Ethic's committee of Guilan University of Medical Sciences
Street address
Gas Sq. Shahid Beheshti Ave.
City
Rasht
Province
Guilan
Postal code
41938-93345
Approval date
2015-12-28, 1394/10/07
Ethics committee reference number
3/132/4313 /پ: شماره - کد IR.GUMS.REC.1394.385
Health conditions studied
1
Description of health condition studied
Sedentary
ICD-10 code
ICD-10 code description
Primary outcomes
1
Description
hs-CRP
Timepoint
24 hours before intervention ( Garlic supplementation ), before the exercise protocol, Immediately after the exercise protocol and 24 hours after the exercise protocol
Method of measurement
Laboratory test techniques ( Seroimmunological method )
2
Description
IL-6
Timepoint
24 hours before intervention ( Garlic supplementation ), before the exercise protocol, Immediately after the exercise protocol and 24 hours after the exercise protocol
Method of measurement
Laboratory test techniques ( ELISA )
3
Description
WBC
Timepoint
24 hours before intervention ( Garlic supplementation ), before the exercise protocol, Immediately after the exercise protocol and 24 hours after the exercise protocol
Method of measurement
Laboratory test techniques ( Use the cell counter device )
4
Description
ALT
Timepoint
24 hours before intervention ( Garlic supplementation ), before the exercise protocol, Immediately after the exercise protocol and 24 hours after the exercise protocol
Method of measurement
Labratorary test techniques ( Enzymatic method with auto analyzer)
5
Description
AST
Timepoint
24 hours before intervention ( Garlic supplementation ), before the exercise protocol, Immediately after the exercise protocol and 24 hours after the exercise protocol
Method of measurement
Labratorary test techniques ( Enzymatic method with auto analyzer)
6
Description
CPK
Timepoint
24 hours before intervention ( Garlic supplementation ), before the exercise protocol, Immediately after the exercise protocol and 24 hours after the exercise protocol
Method of measurement
Labratorary test techniques ( Enzymatic method with auto analyzer)
7
Description
LDH
Timepoint
24 hours before intervention ( Garlic supplementation ), before the exercise protocol, Immediately after the exercise protocol and 24 hours after the exercise protocol
Method of measurement
Labratorary test techniques ( Enzymatic method with auto analyzer)
Secondary outcomes
empty
Intervention groups
1
Description
Intervention group 1: One session intense aerobic exercise. Aerobic exercise including Bruce protocol test on treadmill combine with consumption of garlic tablet 500 mg , twice a day and for 2 weeks
Category
Prevention
2
Description
Intervention group 2: One session intense aerobic exercise. Aerobic exercise including Bruce protocol test on treadmill combine with consumption of lactose capsule 500 mg , twice a day for 2 weeks
Category
Prevention
3
Description
Intervention group 3: One session intense aerobic exercise. Resistance exercises were done by bodybuilding machine as exhausting of 70, 75 and 80% and the rest time for each station was 30 seconds and between each set was 3 minutes combine with consumption of garlic tablet 500 mg , twice a day and for 2 weeks.
Category
Prevention
4
Description
Intervention group 4: One session intense aerobic exercise. Resistance exercises were done by bodybuilding machine as exhausting of 70, 75 and 80% and the rest time for each station was 30 seconds and between each set was 3 minutes combine with consumption of lactose capsule 500 mg , twice a day for 2 weeks.
Category
Prevention
Recruitment centers
1
Recruitment center
Name of recruitment center
Guilan University
Full name of responsible person
Shahram Gholamrezaei darsara
Street address
Amir apartment, Afshin alley, Moin alley, Ansari Ave
City
Rasht
Province
Guilan
Postal code
4163954813
Phone
+98 13 3373 6448
Email
gholamrezaei@iaurasht.ac.ir
Sponsors / Funding sources
1
Sponsor
Name of organization / entity
Guilan University
Full name of responsible person
Shahram Gholamrezaei darsara
Street address
Amir apartment, Afshin ally, Moin alley, Ansari Blvd
City
Rasht
Province
Guilan
Postal code
4163954813
Phone
+98 13 3373 6448
Email
gholamrezaei@iaurasht.ac.ir
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Guilan University
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic
Person responsible for general inquiries
Contact
Name of organization / entity
Islamic Azad University - Rasht Branch
Full name of responsible person
Shahram Gholamrezaei darsara
Position
Associate professor
Latest degree
Ph.D.
Other areas of specialty/work
Exercise physiology
Street address
Drvazeh lakan Ave
City
Rasht
Province
Guilan
Postal code
۴۱۴۷۶۵۴۹۱۹
Phone
+98 13 3342 3153
Fax
Email
gholamrezaei@iaurasht.ac
Web page address
Person responsible for scientific inquiries
Contact
Name of organization / entity
Azad University - Rasht Branch
Full name of responsible person
Shahram Gholamrezaei darsara
Position
Associate professor
Latest degree
Ph.D.
Other areas of specialty/work
Exercise physiology
Street address
Darvazeh lakan Ave
City
Rasht
Province
Guilan
Postal code
414754919
Phone
+98 13 3342 3153
Fax
Email
gholamrezaei@iaurasht.ac
Web page address
Person responsible for updating data
Contact
Name of organization / entity
Azad University - Rasht Branch
Full name of responsible person
Shahram Gholamrezaei darsar
Position
Associate professor
Latest degree
Ph.D.
Other areas of specialty/work
Exercise physiology
Street address
Darvazeh lakan Ave
City
Rasht
Province
Guilan
Postal code
414754919
Phone
+98 13 3342 3153
Fax
Email
gholamrezaei@iaurasht.ac
Web page address
Sharing plan
Deidentified Individual Participant Data Set (IPD)
No - There is not a plan to make this available
Justification/reason for indecision/not sharing IPD
No decision has been made yet.
Study Protocol
Undecided - It is not yet known if there will be a plan to make this available