Drug-induced hypoglycemia is a major obstacle for individuals with type 1 and type 2 diabetes trying to achieve glycemic targets. The episodes of hypoglycemia may increase cardiovascular mortality among patients with or without underlying heart disease. The mechanisms by which hypoglycemia cause increased cardiovascular risk are not clear and are under debate. According to some studies, hyperglycemia increase blood homocysteine and C-reactive protein (CRP) levels as the predisposing factors of cardiovascular disease. However, the effect of hypoglycemia on the level of CRP and homocysteine markers has not been clarified. To determine whether the episodes of hypoglycemia are likely to be associated with cardiovascular disease by increasing blood CRP and homocysteine concentrations, we carried out a study to evaluate blood levels of these two factors in response to insulin-induced hypoglycemic stress. In this regard, fifteen non-diabetic healthy male individuals were enrolled for the study. In all subjects, hypoglycemia was induced by the injection of 0.1 to 0.15 unit/kg intravenous regular insulin. When a blood glucose level dropped below 45 mg/dl, hypoglycemia would be defined to be present. Blood samples were taken from a dedicated intravenous line before the start of test and serially after the injection of insulin in divided times and for 240 minutes. Counter regulatory hormone levels (growth hormone, cortisol, norepinephrine, epinephrine, ACTH), homocysteine, and CRP would be checked at baseline and 30, 45, 60, 120, 240 minutes after insulin injection.