The aim of this study is to investigate the Serum levels of microRNA-16, microRNA-21, microRNA-217 and effects of L-carnitine on their levels, Tumor Necrosis Factor-α, Tumor Growth Factor-β cytokines, Malondialdehyde and C-Reactive Protein in Nonalcoholic Steatohepatitis patients under weight loss regimen. Inclusion criteria: age between 18-65 years; BMI≥25 kg/m2, under weight loss regimen; ALT≥1.5 fold norma;l Sonography showing Nonalcoholic Steatohepatitis, Exclusion criteria: not willing to collaborate;viral hepatitis; taking Insulin ;blood lipid-lowering agents; Antiinflammatory drugs(stroid and non stroid)and antioxidant supplement; smoking cigarettes. In this parallel randomized controlled trial, 92 men and women with NASH. will be recruited from nutrition clinic of Imam Reza hospital of Mashhad University of Medical Science. Blood samples will be obtained after an overnight fast before and after the study. Plasma miRNA will be extracted with Mirvana kit and then Real time PCR will be done. Other variables also will be measured. 3-days 24-recalls will be obtained at first and at the end of the study. Intervention group receives 2000 mg of L-carnitine as 2 vials and other group receives 2 placebo vials,before the meals, for 12 weeks.
agents ,Antiinflammatory drugs(stroid and non stroid)and antioxidant supplement ,smoking cigarettes. Blood samples will be obtained after an overnight fast before and after the study.Plasma miRNA will be extracted with Mirvana kit and then Real time PCR will be done. TNF-α , TGF-β cytokines, Malondialdehyde and CRP also will be measured. 3-days 24-recalls will be obtained at first and at the end of the study. Intervention group receives 2000 mg of L-carnitine as 2 vials and other group receives 2 placebo vials,before the meals, for 12 weeks.