The present research is random study without control by placebo. The statistical population includes all patients admitted to the intensive care unit of Khatam Hospital, affiliated with Zahedan University of Medical Sciences, in 2017. In these patients, patients with multiple trauma, aged 20-60 who were hospitalized in the ICU for at least 48 hours were selected as the sample. The exclusion criteria were affliction with infectious diseases or diabetes, pregnancy (for women), and discharge or transfer to another ward before 48 hours.
Sample size:
Based on previous studies and considering a statistical power of 90% at a confidence level of 95%, the sample size for each of the experimental groups was determined to be 35. To increase the level of confidence and compensate for possible loss of samples, 40 subjects were assigned to each group.
Intervention:
In the control group, sampling will be done using a sterile swab from areas of the armpits, groin, and neck folds on arrival at the hospital and 48 hours after hospitalization without any intervention. Sampling of the same body areas in the intervention group will be conducted with the same method on arrival at the hospital and 48 hours after hospitalization and daily 2% chlorhexidine bath. For bathing, the entire body of patients, except their face, should be treated with a pad containing 2% chlorhexidine solution. Samples will be cultured by the fellow researcher and, 24 hours after incubation, the number of colonies will be counted. Then, samples will be cultured again for determining the type of microorganisms and conduction of antibiotic sensitivity test. Finally, the results of two groups will be compared with each other.