Protocol summary

Study aim
Determination the effect of atorvastatin alone and in combination with vitamin E on Peroxisome Proliferator-Activated Receptor-gamma gene expression in type 2 diabetic patients with hyperlipidemia Determination the effect of atorvastatin alone and in combination with vitamin E on insulin resistance indice (HOMA-IR) in type 2 diabetic patients with hyperlipidemia Determination the effect of atorvastatin alone and in combination with vitamin E on fasting serum insulin in type 2 diabetic patients with hyperlipidemia Determination the effect of atorvastatin alone and in combination with vitamin E on fasting blood glucose in type 2 diabetic patients with hyperlipidemia Determination the effect of atorvastatin alone and in combination with vitamin E on blood lipid profile (triglyceride, total cholesterol, LDL and HDL) in type 2 diabetic patients with hyperlipidemia Determination the effect of atorvastatin alone and in combination with vitamin E on weight loss in type 2 diabetic patients with hyperlipidemia Determination the effect of atorvastatin alone and in combination with vitamin E on waist circumference in type 2 diabetic patients with hyperlipidemia
Design
In this research, 30 eligible type 2 diabetic patients with hyperlipidemia referring to clinics of Vali-e-Asr Hospital were chosen purposefully and a code was allocated to each one of them. Then, patients were randomly divided into two control and intervention groups.
Settings and conduct
This study is a double-blinded (researcher/patient) randomized clinical trial. the aim of the present study is finding the suitable treatment method with lower complications for type 2 diabetic patients with hyperlipidemia. Thirty women with type 2 diabetes which have hyperlipidemia and HbA1c= 7-9% who were attending to the clinics of Vali-e-Asr Hospital will be enrolled. Patients were randomely devided to two equal groups. The first group: recieve atorvastatin 20 mg plus placebo and the second group recieve 20 mg atorvastatin plus 400 IU vitamin E supplement daily for 12 weeks. At the end, results will be compared between the two groups, as well as in each group from baseline up to the end.
Participants/Inclusion and exclusion criteria
Inclusion critera: women with type 2 diabetes who recieve 20 mg per day atorvastatin to control blood lipid; body mass index between 25 to 35 kg/m2; age of 18 to 65 years; glycated hemoglobin of 7 to 9 percent; Exclusion criteria: body mass index lower than 25 kg/m2; pregnant and lactating women; patients with chronic diseases; intake of tiazolidinediones family; intake of supplements at least for 3 month ago; use of alcohol or cigarette; malabsorptive diseases; people on special diets
Intervention groups
Control group: 20 mg atorvastatin plus placebo, daily Intervention group: 20 mg atorvastatin plus 400 IU vitamin E supplement, daily
Main outcome variables
amount of Peroxisome Proliferator-activated receptor-gamma gene expression Insulin resistance indice (HOMA-IR)

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT20170918036256N1
Registration date: 2017-12-15, 1396/09/24
Registration timing: registered_while_recruiting

Last update: 2017-12-15, 1396/09/24
Update count: 0
Registration date
2017-12-15, 1396/09/24
Registrant information
Name
Banafsheh Sadat Tabaei Jabali
Name of organization / entity
Zanjan University of Medical Sciences
Country
Iran (Islamic Republic of)
Phone
+98 24 3344 0300
Email address
tabaei.banafsheh@zums.ac.ir
Recruitment status
Recruitment complete
Funding source
Zanjan University of Medical Sciences
Expected recruitment start date
2017-07-02, 1396/04/11
Expected recruitment end date
2018-01-10, 1396/10/20
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
Comparison of the effect of Atorvastatin alone & in combination with vitamin E on peroxisome proliferator-activated receptor-gamma gene expression and insulin resistance indices in type 2 diabetic patients with hyperlipidemia
Public title
Effect of Atorvastation in combination with vitamin E on treatment of hyperlipidemia
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Inclusion criteria: atorvastatin intake at the dose of 20 mg/day; fasting blood sugar more than 126 mg/dl & two hour plasma glucose more than 200 mg/dl in two regular tests; body mass index between 25 to 35 kg/m2; age of 18 to 65 years; glycated hemoglobin of 7 to 9 percent; Iranian ethnicity Exclusion criteria: bogy mass index lower than 25 kg/m2; pregnant and lactating women or whom decide to pregnancy; chronic diseases such as inflammatory diseases, heart, liver, renal failure, cancer, acute myocardial infarction, type 1 diabetes, stroke & serious injuries; drugs of tiazolidinediones family such as pioglitazone; intake of multivitamin or antioxidant supplements at least for 3 month ago; use of alcohol or cigarette; malabsorptive diseases such as celiac & steatorrhea; liver genetic diseases such as copper or iron storage; athletes; people on special diets
Exclusion criteria:
Age
From 18 years old to 65 years old
Gender
Female
Phase
N/A
Groups that have been masked
  • Participant
  • Care provider
  • Investigator
Sample size
Target sample size: 30
Randomization (investigator's opinion)
Randomized
Randomization description
Simple randomization. By a flip of a coin, the first patient will be considered at the intervention and the second one will be as control group.
Blinding (investigator's opinion)
Double blinded
Blinding description
After collecting the consent form to participate in the study, participants recieved drugs with the same shape and number. Clinical care person and researcher were blinded through coding at the randome allocation.
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Zanjan University of Medical Sciences
Street address
12th street., Mahdavi Blvd., Zanjan University of Medical Sciences
City
Zanjan
Province
Zanjan
Postal code
4515613191
Approval date
2017-05-22, 1396/03/01
Ethics committee reference number
zums.rec.1395.268

Health conditions studied

1

Description of health condition studied
Type 2 diabetes mellitus
ICD-10 code
E11
ICD-10 code description
Non-insulin-dependent diabetes mellitus

Primary outcomes

1

Description
Peroxisome proliferator-activated receptor-gamma gene expression
Timepoint
At the beginning and after 12 weeks
Method of measurement
Polymerase chain reaction

2

Description
Insulin resistance indice
Timepoint
At the beginning and after 12 weeks
Method of measurement
HOMA-IR formula

Secondary outcomes

1

Description
Triglyceride
Timepoint
At the beginning and after 12 weeks
Method of measurement
Autoanalyzer

2

Description
Total cholesterol
Timepoint
At the beginning and after 12 weeks
Method of measurement
Autoanalyzer

3

Description
Low density lipoprotein cholesterol
Timepoint
At the beginning and after 12 weeks
Method of measurement
Autoanalyzer

4

Description
High density lipoprotein cholesterol
Timepoint
At the beginning and after 12 weeks
Method of measurement
Autoanalyzer

5

Description
Fasting glucose
Timepoint
At the beginning and after 12 weeks
Method of measurement
Autoanalyzer

6

Description
Two-hour plasma glucose
Timepoint
At the beginning and after 12 weeks
Method of measurement
Autoanalyzer

7

Description
Insulin resistane
Timepoint
At the beginning and after 12 weeks
Method of measurement
Fasting insulin *fasting glucose/ 405

8

Description
Fasting insulin
Timepoint
At the beginning and after 12 weeks
Method of measurement
ELISA kit

9

Description
Weight reduction
Timepoint
At the beginning and after 12 weeks
Method of measurement
Scale

10

Description
Waist circumference
Timepoint
At the beginning and after 12 weeks
Method of measurement
Meter

11

Description
Glycosylated hemoglobin
Timepoint
At the beginning and after 12 weeks
Method of measurement
Autoanalyzer

Intervention groups

1

Description
Atorvastatin 20 mg (Jalinus Co, Iran) and vitamin E 400 IU (Zahravi Co, Iran), daily after meal, for 12 weeks
Category
Treatment - Drugs

2

Description
Atorvastatin 20 mg (Jalinus Co, Iran) and placebo (containing corn oil, Zahravi Co, Iran), daily after meal, for 12 weeks
Category
Placebo

Recruitment centers

1

Recruitment center
Name of recruitment center
Vali-e-Asr hospital
Full name of responsible person
Maryam Jameshorani
Street address
Vali-e-Asr Ave, Vali-e-Asr hospital
City
Zanjan
Province
Zanjan
Postal code
4515613191
Phone
+98 24 3344 0300
Email
dr.shirinjameshorani@yahoo.com

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Zanjan University of Medical Sciences
Full name of responsible person
Ali Mellati
Street address
12th St, Mahdavi Blv, Zanjan University of Medical Sciences
City
Zanjan
Province
Zanjan
Postal code
4515613191
Phone
+98 24 3344 0300
Email
mellati@zums.ac.ir
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Zanjan University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Zanjan University of Medical Sciences
Full name of responsible person
Banafsheh Sadat Tabaei Jabali
Position
Master student
Latest degree
Bachelor
Other areas of specialty/work
Biochemistry
Street address
12th St, Mahdavi Blv, Zanjan University of Medical Sciences
City
Zanjan
Province
Zanjan
Postal code
4515613191
Phone
+98 24 3344 0300
Fax
Email
tabaei.banafsheh@yahoomail.com
Web page address

Person responsible for scientific inquiries

Contact
Name of organization / entity
Zanjan University of Medical Sciences
Full name of responsible person
Ali Mellati
Position
Professor
Latest degree
Ph.D.
Other areas of specialty/work
Biochemistry
Street address
12th St, Mahdavi Blv, Zanjan University of Medical Sciences
City
Zanjan
Province
Zanjan
Postal code
4515613191
Phone
+98 24 3344 0300
Fax
Email
mellati@zums.ac.ir
Web page address

Person responsible for updating data

Contact
Name of organization / entity
Zanjan University of Medical Sciences
Full name of responsible person
Banafsheh Sadat Tabaei Jabali
Position
Master student of Clinical Biochemistry
Latest degree
Bachelor
Other areas of specialty/work
Biochemistry
Street address
12th St, Mahdavi Blv, Zanjan University of Medical Sciences
City
Zanjan
Province
Zanjan
Postal code
4515613191
Phone
+98 24 3344 0300
Fax
Email
tabaei.banafsheh@zums.ac.ir
Web page address

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Yes - There is a plan to make this available
Study Protocol
Yes - There is a plan to make this available
Statistical Analysis Plan
Yes - There is a plan to make this available
Informed Consent Form
Yes - There is a plan to make this available
Clinical Study Report
Yes - There is a plan to make this available
Analytic Code
Not applicable
Data Dictionary
Not applicable
Title and more details about the data/document
Results of data analysis will be reported.
When the data will become available and for how long
access starts 6 month after publishing the results
To whom data/document is available
Researchers at the universities
Under which criteria data/document could be used
Useless
From where data/document is obtainable
E-mail tabaei.banafsheh@zums.ac.ir
What processes are involved for a request to access data/document
Electronic mail
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