Protocol summary

Summary
Objective: Safety and efficacy assessment (such as quality of life and hip joint condition improvement and pain relief) of bone marrow derived CD133+ cell transplantation in patients with avascular necrosis of femoral head. Design: An intervention clinical trial phase I, not blinded, non randomized, uni-center in patients with avascular necrosis of femoral head. Sample size: Ten patients. Setting and conduct: Patients with osteonecrosis of the femoral head who visited in orthopedics clinic of Royan cell therapy center, would transplant autologous bone marrow derived CD133+ cells during core decompression surgery and they will follow at 2, 6 and 12 months after injection. Participants: Patients in a range of 18 to 65 years old, with femoral head osteonecrosis who are candidates for core decompression, and not suffered from other chronic diseases, viral disorders and cancer. Interventions: Implantation of autologous bone marrow derived CD133 possesses cells with core decompression procedure. Primary outcome: Safety of bone marrow derived CD133+ cell transplantation Secondary outcome: Efficacy assessment (such as quality of life and hip joint condition improvement and pain relief) of bone marrow derived CD133+ cell transplantation

General information

Acronym
IRCT registration information
IRCT registration number: IRCT201507231031N3
Registration date: 2016-10-06, 1395/07/15
Registration timing: retrospective

Last update:
Update count: 0
Registration date
2016-10-06, 1395/07/15
Registrant information
Name
Nasser Aghdami
Name of organization / entity
Royan Institute
Country
Iran (Islamic Republic of)
Phone
+98 21 2356 2000
Email address
nasser.aghdami@royaninstitute.org
Recruitment status
Recruitment complete
Funding source
Department of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR
Expected recruitment start date
2009-07-23, 1388/05/01
Expected recruitment end date
2013-05-22, 1392/03/01
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
Evaluation of safety and feasibility of bone marrow-derived CD133 cells possesses transplantation in patients with the femoral head Osteonecrosis (AVN) -An interventional clinical trial phase I
Public title
Transplantation of bone marrow cells in patients with femoral head osteonecrosis
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion Criteria: Upenn (Steinberg) classification of osteonecrosis, inclusive of Stages IIB and IIC that diagnosis will be based on magnetic resonance imaging (MRI); Modified index of necrotic extent < 40; Idiopathic and non-idiopathic osteonecrosis; No infection in affected bones at the time of surgery; Patient competent to give informed consent; Normal organ and marrow function defined as: Leukocytes ≥ 3000/µL; Absolute neutrophil count ≥ 1500/µL; Platelets ≥ 140,000/µL; Serum AST (SGOT)/ALT (SGPT) < 2.5 X institutional standard range; Serum creatinine within normal limits, based on clinical laboratory normal range; Female patients not pregnant or lactating; Patients with a history of corticosteroids or on active therapy, will only be eligible for enrollment if corticosteroid use is suspended for 1 month prior and 6 months after cell therapy and surgery; Patients who have been treated with oral bisphosphonates are eligible for the trial if treatment was stopped at least 6 months prior to enrollment. Exclusion Criteria: Femoral head osteonecrosis' Stages IA, IB, IC, IIA, IIIA or more severe, primarily based on diagnosis by MRI; Flattening of the femur head (UPenn Stage IV) or articular cartilage collapse while core decompression surgery; Septic arthritis; stress fracture, or non-osteonecrosis metabolic bone diseases (e.g., Paget's disease of bone, osteogenesis imperfecta, primary hyperparathyroidism, fibrous dysplasia [monostotic, polyostotic McCune-Albright syndrome] and osteopetrosis); Any active bisphosphonate treatment or any history of intravenous (IV) treatment; HIV, syphilis, positive at time of screening; Active hepatitis B or hepatitis C infection at the time of screening; Known allergies to protein products (horse or bovine serum, or porcine trypsin); Patients who will require continuous, systemic, high dose corticosteroid therapy (more than 7.5 mg/day) within 6 months after surgery; received chemotherapy, radiotherapy or immunotherapy in the past 2 years; Immunodeficiency diseases; Participation in another clinical study in the past 30 days or concurrent participation in another clinical trial; History of regular alcohol consumption exceeding 2 drinks/day (1 drink = 5 oz [150 mL] of wine or 12 oz [360 mL] of beer or 1.5 oz [45 mL] of hard liquor) within 6 months of screening and/or history of illicit drug use; MRI-incompatible internal devices (pacemakers, aneurysm clips, etc); Body mass index (BMI) of 40 Kg/m2 or greater; Patients unable to tolerate general anesthesia defined as an American Society of Anesthesiologists (ASA) criteria of > 2; Patients with poorly controlled diabetes mellitus (HbA1C > 8%), or with peripheral neuropathy, or known concomitant vascular problems; Patients receiving treatment with hematopoietic growth factors or anti-vasculogenesis or anti-angiogenesis treatment; Traumatic osteonecrosis
Age
From 18 years old to 65 years old
Gender
Both
Phase
1
Groups that have been masked
No information
Sample size
Target sample size: 10
Randomization (investigator's opinion)
Not randomized
Randomization description
Blinding (investigator's opinion)
Not blinded
Blinding description
Placebo
Not used
Assignment
Single
Other design features

Secondary Ids

1

Registry name
clinicaltrials.gov
Secondary trial Id
NCT01198080
Registration date
2010-08-09, 1389/05/18

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Royan infertility institute
Street address
Eastern Hafez St,Northern Banihashem St, Resalat highway 45 meters
City
Tehran
Postal code
Approval date
2011-05-10, 1390/02/20
Ethics committee reference number
EC/90/1028

Health conditions studied

1

Description of health condition studied
Osteonecrosis of the femoral head, unspecified
ICD-10 code
M87.9
ICD-10 code description
Osteonecrosis, unspecified

Primary outcomes

1

Description
safety of bone marrow derived CD133+ cells transplantation
Timepoint
1, 3, 6 and 12 months after transplantation
Method of measurement
MRI, Physical function, side effects

Secondary outcomes

1

Description
Quality of life
Timepoint
Before injection, 6 and 12 months after injection
Method of measurement
SF-36 Questionnaire

2

Description
Hip articular change
Timepoint
Before injection, 6 and 12 months after injection
Method of measurement
Standard WOMAC, VAS and HHS questionnaires

3

Description
Side effects
Timepoint
Before injection, 6, 12 and 24 months after injection
Method of measurement
Side effects questionnaire

Intervention groups

1

Description
Intervention group: bone marrow derived CD133+ cells injection with core compression
Category
Other

Recruitment centers

1

Recruitment center
Name of recruitment center
Orthopedic Clinic of Royan Cell Theraoy Center
Full name of responsible person
Fateme Shams
Street address
Royan Institute,Eastern Hafez St,Northern Banihashem St, Resalat highway 45 meters
City
Tehran

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Royan Institute
Full name of responsible person
Hamid Gourabi
Street address
Eastern Hafez St,Northern Banihashem St, Resalat highway 45 meters
City
Tehran
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Royan Institute
Proportion provided by this source
100
Public or private sector
empty
Domestic or foreign origin
empty
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
empty

Person responsible for general inquiries

Contact
Name of organization / entity
Royan Institute
Full name of responsible person
Shahedeh Karimi Torshizi
Position
M.D/ Researcher
Other areas of specialty/work
Street address
Royan institute, Eastern Hafez St, Northern Banihashem St, 45 m Resalat highway
City
Tehran
Postal code
Phone
+98 21 2251 8388
Fax
Email
karimi58shah@gmail.com
Web page address

Person responsible for scientific inquiries

Contact
Name of organization / entity
Royan cell Therapy Center
Full name of responsible person
Nasser Aghdami
Position
MD, PhD
Other areas of specialty/work
Street address
Royan Institute, Eastern Hafez St,Northern Banihashem St, 45 m Resalat highway
City
Tehran
Postal code
Phone
+98 21 2356 2000
Fax
Email
nasser.aghdami@royaninstitute.org
Web page address

Person responsible for updating data

Contact
Name of organization / entity
Royan Institute
Full name of responsible person
Hoda Madani
Position
researcher
Other areas of specialty/work
Street address
Royan Institute, Eastern Hafez St,Northern Banihashem St, Resalat highway 45 meters
City
Tehran
Postal code
Phone
+98 21 2251 8388
Fax
+98 21 2251 6465
Email
hoda62_m@yahoo.com
Web page address

Sharing plan

Deidentified Individual Participant Data Set (IPD)
empty
Study Protocol
empty
Statistical Analysis Plan
empty
Informed Consent Form
empty
Clinical Study Report
empty
Analytic Code
empty
Data Dictionary
empty
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