Protocol summary

Summary
This is a single-blind controlled trial on the possible effects of vitamin D receptor (VDR) polymorphisms on efficacy of vitamin D intake in the patients with type 2 diabetes mellitus (T2DM). Ninety T2DM patients will receive 250 ml yogurt drink fortified with 500 IU of vitamin D3 twice a day for 3 months. VDR Fok1 genotypes will be determined at the end of the study and the responses to vitamin D (metabolic, inflammatory and oxidative stress) will be compared between the three subgroups, i.e. FF, Ff and ff. To relate the possible changes in the indicators to the vitamin D intake, there will be a diabetic control group (n=30) who will receive the same amount of plain (unfortified) yogurt drink during the study period. The responses of the control group will be compared with those of 30 diabetic patients randomly selected from the fortified yogurt drink group.

General information

Acronym
IRCT registration information
IRCT registration number: IRCT138810223006N2
Registration date: 2010-04-27, 1389/02/07
Registration timing: retrospective

Last update:
Update count: 0
Registration date
2010-04-27, 1389/02/07
Registrant information
Name
Tirang Reza Neyestani
Name of organization / entity
National nutrition and food technology research institute
Country
Iran (Islamic Republic of)
Phone
+98 21 2237 6471
Email address
t.neyestani@nnftri.ac.ir
Recruitment status
Recruitment complete
Funding source
1- National Nutrition & Food technology Research Institute , Shaheed Beheshti University of Medical Sciences & Health Services 2- School of Public health, Tehran University of Medical Sciences
Expected recruitment start date
2009-12-11, 1388/09/20
Expected recruitment end date
2010-03-20, 1388/12/29
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
The role of vitamin D receptor (VDR) single nucleotide polymorphisms (SNPs ) in efficacy of vitamin D-fortified yogurt drink on metabolic-, inflammatory – and oxidative stress indicators in type 2 diabetic patients
Public title
Vitamin D receptor polymorphism , vitamin D-fortified Yogurt drink, Type 2 Diabetes
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria: 1. Fasting blood glucose ≥ 126, 2. BMI between 25 to 35 kg/m2, 3. Age: 30-60 years old, 4. Willingness to maintain current body weight for the duration of the study, 5. Willingness to maintain baseline lifestyle activities and routines for the duration of the study, 6. Willingness to avoid the use of all over-the-counter or prescription vitamins, dietary supplements, and herbal products during the study. Exclusion criteria: 1. History of cardiovascular disease, Gastrointestinal disease, Renal disease, Kidney stone, Hyperparathyroidism, 2. Receiving vitamin D or omega-3 supplement within the last three months, 3. Pregnancy , or lactation within the study period, 4. Receiving insulin or any drug for weight reduction, reducing serum cholesterol, 5. Any change in type /or dose of the current hypoglycemic medications,
Age
From 30 years old to 60 years old
Gender
Both
Phase
2
Groups that have been masked
No information
Sample size
Target sample size: 120
Randomization (investigator's opinion)
Not randomized
Randomization description
Blinding (investigator's opinion)
Single blinded
Blinding description
Placebo
Not used
Assignment
Other
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethical Committee of National Nutrition & Food technology Research Institute
Street address
No 46, Arghavan Gharbi, Farahzadi Blvd., Shahrak Qods (Gharb)
City
Tehran
Postal code
1981619573
Approval date
empty
Ethics committee reference number
035360

Health conditions studied

1

Description of health condition studied
Type 2 Diabetes
ICD-10 code
E11
ICD-10 code description
Non-insulin-dependent diabetes mellitus

Primary outcomes

1

Description
Improvment in Serum Vitamin D Status
Timepoint
3 Months
Method of measurement
Serum 25(OH)D Level

Secondary outcomes

1

Description
oxidative stress biomarkers
Timepoint
baseline and 3 month after intervention
Method of measurement
serum superoxide dismutase,glutathion, glutathion peroxidase, MDA, TAC

2

Description
inflammatory biomarkers
Timepoint
Baseline and 3 month after intervention
Method of measurement
Serum CRP,IL-6 and Il-4 , TNF-α and IFN-γ concentrations in cell culture medium

3

Description
bone biomarkers
Timepoint
baseline and 3 month after intervention
Method of measurement
serum magnesium,Phosphor, calcium, PTH,Osteocalcin

4

Description
glycemic biomarkers
Timepoint
baseline and 3 month after intervention
Method of measurement
serum fasting glucose ,Insulin, HbA1C and Quiccki index

5

Description
anthropometric indicators
Timepoint
Baseline and 3 month after intervention
Method of measurement
Weight, Height, BMI,Hip Circumferences ، Waist Circumferences, WHR

6

Description
albumin, total protein, BUN, AST, ALT, lipid profiles and uric acid
Timepoint
Baseline and 3 months after intervention
Method of measurement
Serum Albumin, Total protein, BUN, AST, ALT, Lipid profiles and Acid uric

7

Description
urinary albumin, calcium, magnesium,phosphorous and micro-albumin
Timepoint
baseline and 3 month after intervention
Method of measurement
Urinary concentrations of micro-albumin, calcium, magnesium and phosphorous

8

Description
blood pressure
Timepoint
baseline and 3 month after intervention
Method of measurement
systolic and diastolic blood pressure

Intervention groups

1

Description
Intervention Group: Yogurt drink Fortified with 500 IU Cholecalciferol per 250 ml in AA genotype subgroup
Category
Treatment - Other

2

Description
Intervention Group: Yogurt drink fortified with 500 IU cholecalciferol per 250 ml in aa genotype subgroup
Category
Treatment - Other

3

Description
Intervention Group: Yogurt drink fortified with 500 IU cholecalciferol per 250 ml in Aa genotype subgroup
Category
Treatment - Other

4

Description
Control Group: Plain yogurt drink
Category
Placebo

Recruitment centers

1

Recruitment center
Name of recruitment center
Iranian Diabetes Scociety
Full name of responsible person
Street address
46 Ramin Malakooti Street , Patris Lomumba
City
Tehran

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
National Nutrition & Food Technology Research Institute, Shaheed Beheshti University of Medical Scie
Full name of responsible person
Dr Tirang Reza Neyestani
Street address
Number 46, West Arghavan St., Farahzad Blvd.,Shahrak Qods
City
Tehran
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
National Nutrition & Food Technology Research Institute, Shaheed Beheshti University of Medical Scie
Proportion provided by this source
Public or private sector
empty
Domestic or foreign origin
empty
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
empty

2

Sponsor
Name of organization / entity
School of Public Health, Tehran University of Medical Sciences
Full name of responsible person
Dr Abolghasem Djazayeri
Street address
Nutrition & Biochemistry Departement, School of Public Health, Tehran University of Medical Sciences
City
Tehran
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
School of Public Health, Tehran University of Medical Sciences
Proportion provided by this source
Public or private sector
empty
Domestic or foreign origin
empty
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
empty

Person responsible for general inquiries

Contact
Name of organization / entity
Public Health School,Tehran University of Medical Sciences
Full name of responsible person
Sakineh Shab-Bidar
Position
Ph.D. Student in Nutritional Sciences
Other areas of specialty/work
Street address
Department of Nutrition & Biochemistry, School of Public Health,Tehran University of Medical Sciences
City
Tehran
Postal code
Phone
+98 21 6640 2095
Fax
+98 21 8897 4462
Email
s.shabbidar@yahoo.com ; s_shabbidar@razi.tums.ac.ir
Web page address

Person responsible for scientific inquiries

Contact
Name of organization / entity
National Nutrition and Food Technology Research Institute, Shaheed Beheshti University of Medical Sc
Full name of responsible person
Dr Tirang Reza Neyestani
Position
Ph.D. in Nutrition
Other areas of specialty/work
Street address
Number 46, West Arghavan St.,Farahzad Blvd.,Shahrak Qods,zip Code:1981619573
City
Tehran
Postal code
1981619573
Phone
+98 21 2237 6471
Fax
+98 21 2236 0660
Email
neytr@yahoo.com ; t.neyestani@nnftri.ac.ir
Web page address

Person responsible for updating data

Contact
Name of organization / entity
Scoohl of Public Health, Tehran University of medical sciences
Full name of responsible person
Sakineh Shab-bidar
Position
Ph.D. student in nutrition
Other areas of specialty/work
Street address
Nutrition & Biochemistry departement, School of Public Health, Tehran University of Medical Sciences
City
Tehran
Postal code
Phone
+98 21 2237 6471
Fax
+98 21 2236 0660
Email
s.shabbidar@yahoo.com ; s_shabbidar@razi.tums.ac.ir
Web page address

Sharing plan

Deidentified Individual Participant Data Set (IPD)
empty
Study Protocol
empty
Statistical Analysis Plan
empty
Informed Consent Form
empty
Clinical Study Report
empty
Analytic Code
empty
Data Dictionary
empty
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