Investigate the effect of alpha lipoic acid supplementation on serum level of LP-PLA2, it`s distribution between HDL and apo B containing lipoproteins and related oxidative stress and inflammatory markers in type 2 diabetic patients

Determination of the effect of alpha lipoic acid supplementation on serum level of lipoprotein-dependent phospholipase A2 and related inflammatory and oxidative stress markers in type 2 diabetic patients

A Randomized, Placebo-Controlled, Double-Blind, Parallel-Group, Clinical trial

Patients with type 2 diabetes who come to the Diabetes Clinic of Tehran University of Medical Sciences who have inclusion criteria will be included in the study. These patients will be randomized into two intervention and control groups for 2 months. The supplement and placebo are named by a third person in two forms A and B, and the researcher, analyst, and participants will be unaware of this categorization.

Inclusion Criteria: Willingness to cooperate; Age between 40-60 years old; Body mass index (BMI) between 18.5 to 29.9; At least 2 years of diagnosed type 2 diabetes; Controlled Diabetes (HbA1C <7%). Non-inclusion criteria: Taking Insulin; History of angina, myocardial infarction, and stroke, as well as other chronic diseases and infectious diseases in the past year; Tobacco and alcohol consumption in the last three months; Changes in treatment protocols in the last three months; taking any medicine other than metformin, glibenclamide, statin drugs, ACEI, ARB and doses less than 80 mg aspirin; Regular use of herbal Supplements containing antioxidants and omega-3 in the last three months (at least once a week); Pregnancy and lactation

Patients in the intervention group received 1200 mg of alpha-lipoic acid supplement per day, and in the control group received placebo capsules (maltodextrin) for 2 months.

Lp-PLA2 and its lipoprotein distribution, ICAM-1, VCAM-1, TNF-a, IL-6, 8-isoproprane, and OX-LDL and APO A1

In this study, diabetic patients will be randomly (block randomization) assigned to two intervention and control groups based on sex and menopausal (stratified randomization). We will create four blocks of letters T (treatment) and C (control), such that half the letters of each block T and the other half C (eg TTCC, CTTC, etc.). The blocks will be created and numbered then we will randomly select the blocks needed to reach the sample size and we will put them together. Based on the created pattern, patients will be assigned to two intervention and control groups

The supplements and placebos are similar in terms of shape, odor, taste, and packaging, and are labeled by a third person (from experts in the diabetes clinic) in two forms A and B.Patients, researcher and analyst would not know who was receiving the supplements and who was receiving a placebo.