Protocol summary

Summary
This double blind randomized controlled clinical trial study was designed to investigate the effects of zinc supplementation on serum adiponectin level and insulin resistance in patients with type II diabetes mellitus compared with the control group. Total of 60 patients aged between 30 to 50 years taking antidiabetic drugs, such as Metformin or Glibenclamid, are selected from Kashan Diabetic Center, and are randomly allocated into equal groups of placebo and zinc. One group will receive 30 mg zinc gluconate daily and other receive the same tablets as placebo for 12 weeks. Diets, exercise level and medications dosage will be held constant throughout the study. After obtaining the informed consent, subjects will be assessed by dietary intake, anthropometric, clinical and medical history, and biochemical measurements at the beginning and the end of the study.

General information

Acronym
IRCT registration information
IRCT registration number: IRCT138811013128N1
Registration date: 2010-11-13, 1389/08/22
Registration timing: retrospective

Last update:
Update count: 0
Registration date
2010-11-13, 1389/08/22
Registrant information
Name
Somayyeh Asghari mahiabadi
Name of organization / entity
Tehran University of Medical Sciences
Country
Iran (Islamic Republic of)
Phone
+98 26 1253 0338
Email address
sasghari@razi.tums.ac.ir
Recruitment status
Recruitment complete
Funding source
Endocrinology & Metabolism Research Center, Tehran University of Medical Sciences
Expected recruitment start date
2009-10-23, 1388/08/01
Expected recruitment end date
2010-03-16, 1388/12/25
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
The effects of zinc supplementation on serum adiponectin level and insulin resistance in patients with type II diabetes mellitus
Public title
The effects of zinc supplementation on serum adiponectin level and insulin resistance in patients with type II diabetes mellitus
Purpose
Prevention
Inclusion/Exclusion criteria
Inclusion criteria: Age between 30 to 50 years, presence of type 2 diabetes at least for one year, willingness to participate in the study and sign the informed consent, BMI=25-30, HbA1C≤8.5, Blood pressure ≤135/85 mmHg. Exclusion criteria: severe kidney, heart, liver, GI, pulmonary and infectious diseases, thyroid dysfunction, active cancer, previous myocardial infarction, recent surgery, Insulin therapy, changing in dose of medications such as oral anti-diabetic, anti-lipidemic and anti-hypertensive drugs 3 month before and during the study, consumption of vitamin-mineral supplements 6 month before and during the study, consumption of non-steroidal anti inflammatory drugs, glucocorticoids, hormonal drugs, antidepressants, psychotropic medications, antibiotics, dioretics, three months prior to study, Following weight-reducing diet 3 months before and during the study, smoking, pregnancy, breast feeding, postmenopausal women.
Age
From 30 years old to 50 years old
Gender
Both
Phase
2-3
Groups that have been masked
No information
Sample size
Target sample size: 60
Randomization (investigator's opinion)
Randomized
Randomization description
Blinding (investigator's opinion)
Double blinded
Blinding description
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Endocrinology & Metabolism Research Center
Street address
Shariati hospital,Tehran University of Medical Sciences
City
Tehran
Postal code
Approval date
2010-09-04, 1389/06/13
Ethics committee reference number
E-0097

Health conditions studied

1

Description of health condition studied
diabetes mellitus type 2
ICD-10 code
E11
ICD-10 code description
Non-insulin dependent diabetes mellitus

Primary outcomes

1

Description
plasma adiponectin
Timepoint
Baseline and after 12 weeks
Method of measurement
ELISA assay

Secondary outcomes

1

Description
Fasting blood glucose
Timepoint
Baseline and after 12 weeks
Method of measurement
Glucose oxidase, Enzymatic colorimetric

2

Description
Fasting insulin serum
Timepoint
Baseline and after 12 weeks
Method of measurement
ELISA assay

3

Description
HbA1C (Glycosilated hemoglobin)
Timepoint
Baseline and after 12 weeks
Method of measurement
Ion exchange method

4

Description
Insulin resistance
Timepoint
Baseline and after 12 weeks
Method of measurement
HOMA-IR calculation

5

Description
Serum triglyceride
Timepoint
Baseline and after 12 weeks
Method of measurement
Glycerol phosphate oxidase, Enzymatic colorimetric

6

Description
Total cholestrol
Timepoint
Baseline and after 12 weeks
Method of measurement
Enzymatic photometric

7

Description
HDL-cholestrol
Timepoint
Baseline and after 12 weeks
Method of measurement
Enzymatic

8

Description
LDL-cholestrol
Timepoint
Baseline and after 12 weeks
Method of measurement
Enzymatic

9

Description
hsC-Reactive Protein
Timepoint
Baseline and after 12 weeks
Method of measurement
ELISA assay

10

Description
serum zinc
Timepoint
Baseline and after 12 weeks
Method of measurement
atomic absorption spectrophotometery

Intervention groups

1

Description
30 mg zinc (zinc gluconate) daily
Category
Treatment - Drugs

2

Description
one plasebo tablet daily
Category
Placebo

Recruitment centers

1

Recruitment center
Name of recruitment center
Kashan Diabetic Center (Golabchi Clinic)
Full name of responsible person
Somayyeh Asghari
Street address
Emam street
City
Kashan

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Endocrinology & Metabolism Research Center
Full name of responsible person
Ramin Heshmat
Street address
Shariati Hospital, Tehran University of Medical Sciences
City
Tehran
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Endocrinology & Metabolism Research Center
Proportion provided by this source
100
Public or private sector
empty
Domestic or foreign origin
empty
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
empty

Person responsible for general inquiries

Contact
Name of organization / entity
Nutrition and Biochemistry Department, School of Health, Tehran University of Medical Sciences
Full name of responsible person
Somayyeh Asghari
Position
Master Degree
Other areas of specialty/work
Street address
Tehran University of Medical Sciences
City
Tehran
Postal code
Phone
+98 21 8897 3655
Fax
Email
asghari.nut@gmail.com
Web page address

Person responsible for scientific inquiries

Contact
Name of organization / entity
Nutrition and Biochemistry Department, School of Health, Tehran University of Medical Sciences
Full name of responsible person
Mohammad Javad Hoseinzadeh
Position
MD.PhD
Other areas of specialty/work
Street address
Tehran University of Medical Sciences
City
Tehran
Postal code
Phone
+98 21 8895 1395
Fax
Email
hosseinzadeh.MDPhD@yahoo.com
Web page address

Person responsible for updating data

Contact
Name of organization / entity
Nutrition and Biochemistry Department, School of Health, Tehran University of Medical Sciences
Full name of responsible person
Somayyeh Asghari
Position
Master Degree
Other areas of specialty/work
Street address
Tehran University of Medical Sciences
City
Tehran
Postal code
Phone
+98 21 8897 3655
Fax
Email
asghari.nut@gmail.com
Web page address

Sharing plan

Deidentified Individual Participant Data Set (IPD)
empty
Study Protocol
empty
Statistical Analysis Plan
empty
Informed Consent Form
empty
Clinical Study Report
empty
Analytic Code
empty
Data Dictionary
empty
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