Protocol summary

Study aim
The effect of aquatic exercise, Vitamin D supplementation and interactive of aquatic exercise and Vitamin D supplementation on serum level of IL-1B, IL-6, IL-10, IL-1ra, TNF-a, hs-CRP and Vitamin D in Children with Autism Spectrum Disorders
Design
Randomised, factorial design and double blinded trial, with three intervention and one control group, Selection of available samples and randomised replacement in groups
Settings and conduct
execution of Blood sampling at Razi lab aquatic Exercise in the Behzisty pool of Rasht Vitamin D supplements based on the prescription of pediatric neurosurgeons Participants, Blood sampling, Laboratory scientist and Expert statistician, except the researcher and his aides, did not know the grouping of people in the research groups
Participants/Inclusion and exclusion criteria
sex (males) age (6 to 14) not having co-morbid disability parents satisfaction and presence
Intervention groups
aquatic exercise vitamin D supplementation interaction of aquatic exercise and vitamin D supplementation control
Main outcome variables
Improving IL-6, IL-10; IL-1b; IL-1ra, TNF-a; hs-CRP; vitamin D serum levels

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT20180503039517N1
Registration date: 2018-07-13, 1397/04/22
Registration timing: retrospective

Last update: 2018-07-13, 1397/04/22
Update count: 0
Registration date
2018-07-13, 1397/04/22
Registrant information
Name
Soleyman Ansari Kolachahi
Name of organization / entity
Country
Iran (Islamic Republic of)
Phone
+98 13 3375 2906
Email address
solomonansari@phd.iaurasht.ac.ir
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2017-12-22, 1396/10/01
Expected recruitment end date
2018-01-20, 1396/10/30
Actual recruitment start date
2017-12-22, 1396/10/01
Actual recruitment end date
2018-03-11, 1396/12/20
Trial completion date
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Scientific title
The effect of aquatic exercise, Vitamin D supplementation and interactive of aquatic exercise and Vitamin D supplementation on serum level of IL-1B, IL-6, IL-10, IL-1ra, TNF-a, hs-CRP and Vitamin D in Children with Autism Spectrum Disorders
Public title
The effect of interactive of aquatic exercise and Vitamin D supplementation on some inflammatory Cytokines, hs-CRP and Vitamin D levels in Children with Autism Spectrum Disorders
Purpose
Supportive
Inclusion/Exclusion criteria
Inclusion criteria:
age (6 to 14) sex (male) not having co-morbid disability
Exclusion criteria:
Having more than two absence in the treatment sessions Receiving specialized treatment in addition to the usual treatments at their institution
Age
From 6 years old to 14 years old
Gender
Male
Phase
N/A
Groups that have been masked
  • Participant
  • Outcome assessor
  • Data analyser
Sample size
Target sample size: 40
Actual sample size reached: 40
Randomization (investigator's opinion)
Randomized
Randomization description
40 children aged 6-14 years with autism spectrum disorder were selected using available sampling method. They will randomly divided into three intervention groups and one control group.
Blinding (investigator's opinion)
Double blinded
Blinding description
In this research, participants are unaware of the group assigned to themselves and other participants. A separate workout hour is considered for each intervention group. experts in laboratory analysis are also unaware of the type of intervention and the participating groups.
Placebo
Not used
Assignment
Factorial
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Rasht Azad University Medical Sciences
Street address
Azad University of Rasht, Lakan Ave
City
Rasht
Province
Guilan
Postal code
4147654919
Approval date
2017-09-19, 1396/06/28
Ethics committee reference number
IR.IAU.RASHT.REC.1396.99

Health conditions studied

1

Description of health condition studied
Autism Spectrum Disorder
ICD-10 code
F84.0
ICD-10 code description
Childhood autism

Primary outcomes

1

Description
IL-1B; IL-6; IL-10; IL-1ra; TNF-a; vitamin D, hs-CRP
Timepoint
One week before starting and on week after terminating of intervention
Method of measurement
Diaclone and ZellBio human kit

Secondary outcomes

empty

Intervention groups

1

Description
Intervention group: Intervention group: aquatic exercise (10 weeks, 2 session per week, 60 min training in pool)
Category
Lifestyle

2

Description
Intervention group: vitamin D supplementation (for 10 weeks, once a week orally, along with having dinner, 500000 IU vitamin D)
Category
Lifestyle

3

Description
Intervention group: composition of aquatic exercise and vitamin D supplementation (combining of intervention 1 and 2)
Category
Lifestyle

4

Description
Control group: no intervention, continuing past life style, not including in new activity
Category
Lifestyle

Recruitment centers

1

Recruitment center
Name of recruitment center
Guilan Autism Association
Full name of responsible person
Mehdi Zahedi Kalashtari
Street address
second Fooladloo, Saffari Ave, Bisoton Ave
City
Rasht
Province
Guilan
Postal code
4193191933
Phone
+98 13 3351 3235
Email
Z_hoj@yahoo.com

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Islamic Azad University
Full name of responsible person
Zahra Hojjati
Street address
Azad University of Rasht, Lakan Ave
City
Rasht
Province
Guilan
Postal code
۴۱۴۷۶۵۴۹۱۹
Phone
+98 13 3342 4073
Email
Z_hoj@yahoo.com
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Islamic Azad University
Proportion provided by this source
100
Public or private sector
Private
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Islamic Azad University
Full name of responsible person
Soleiman Ansari Kolachahi
Position
Phd student
Latest degree
Master
Other areas of specialty/work
Biochemistry
Street address
Ansar Sport Club, 6th Ave, Keshavarz Ave, Valiasr Ave
City
Rasht
Province
Guilan
Postal code
4139617379
Phone
+98 13 3375 2906
Email
solomonansari@phd.iaurasht.ac.ir

Person responsible for scientific inquiries

Contact
Name of organization / entity
Islamic Azad University
Full name of responsible person
Soleiman Ansari Kolachahi
Position
Phd student
Latest degree
Master
Other areas of specialty/work
Biochemistry
Street address
Ansar Sport Club, 6th Ave, Keshavarz Ave, Valiasr Ave
City
Rasht
Province
Guilan
Postal code
4139617379
Phone
+98 13 3375 2906
Email
solomonansari@phd.iaurasht.ac.ir

Person responsible for updating data

Contact
Name of organization / entity
Islamic Azad University
Full name of responsible person
Soleiman Ansari Kolachahi
Position
Phd student
Latest degree
Master
Other areas of specialty/work
Biochemistry
Street address
Ansar Sport Club, 6th Ave, Keshavarz Ave, Valiasr Ave
City
Rasht
Province
Guilan
Postal code
4139617379
Phone
+98 13 3375 2906
Email
solomonansari@phd.iaurasht.ac.ir

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Undecided - It is not yet known if there will be a plan to make this available
Study Protocol
Undecided - It is not yet known if there will be a plan to make this available
Statistical Analysis Plan
No - There is not a plan to make this available
Informed Consent Form
Undecided - It is not yet known if there will be a plan to make this available
Clinical Study Report
Not applicable
Analytic Code
Not applicable
Data Dictionary
Not applicable
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