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Study aim
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The purpose of this study was to determine changes in the expression profile of cumulus cells in genes under the influence of different pre-incubation time of human oocytes in patients undergoing assisted reproductive cycles.
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Design
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In this study, the participants were randomly allocated to one of the two groups of zero and three hours of oocyte pre- incubation based on random numbers obtained from internet sites. The number of people in each group is 10, which obtain according to a study in 2009, taking into account the first type error, α = 0.05 and the second type error was β = 10%.
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Settings and conduct
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This study is conducted at Akbar Abadi Hospital's Infertility Clinic. Participants will be randomly assigned to one of the groups after receiving sufficient information about the study groups and the consent form, without being informed of their study group.
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Participants/Inclusion and exclusion criteria
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Inclusion criteria: Age aged 25 to 35 years, patients who are in the agonist cycle, infertile women with tubal problems, normal semen analysis according to WHO 2010 criteria
Exclusion criteria: pelvic acute infection, coagulation disorder, cervicitis and vaginitis, cervix malignancy, BMI>35, endometriosis (grade 3 &4), polycystic ovarian syndrome, oocyte number less 2 and further 15
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Intervention groups
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Intervention group 1: The oocytes are not affected by pre- incubation.
Intervention group 2: The oocytes are affected by pre-incubation for 3 hours.
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Main outcome variables
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Primary outcome variable: Determine the preincubation time appropriate for the human ovum, according to the molecular results for approaching the highest fertility and embryo quality.
Secondary outcome variable: The determination of gene expression profiles that are involved in these pathways and can serve as a marker for the quality of oocytes and embryos.