Protocol summary

Summary
The aim of this randomized double blind clinical trial is to determine the effects of probiotic yoghurt consumption on some blood biochemical parameters in type 2 diabetic patients. Sixty patients aged between 30-60 years old from both sexes are assigned into two groups. Patients in intervention group consume 300 gr/day probiotic yoghurt and patients in control group consume 300 gr/day conventional yoghurt for 6 weeks. Anthropometric and blood pressure measurements, 3-day 24h dietary recalls and fasting blood samples are collected at the beginning and at the end of study. Fasting blood sugar, glycosylated hemoglobin, insulin, lipid profile, malondialdehyde (MDA), total antioxidant capacity (TAC), catalase, superoxide dismutase, glutathione peroxidase and hsCRP are measured. LDL and HOMA-IR are calculated by formula. The participants are asked not to change their regular diet, medicine and activity during the study and not to consume any other yoghurt.

General information

Acronym
IRCT registration information
IRCT registration number: IRCT138903223533N1
Registration date: 2010-07-03, 1389/04/12
Registration timing: registered_while_recruiting

Last update:
Update count: 0
Registration date
2010-07-03, 1389/04/12
Registrant information
Name
Javad Mohtadinia
Name of organization / entity
Faculty of health and nutrition
Country
Iran (Islamic Republic of)
Phone
+98 41 1335 7581
Email address
mohtadiniaj@tbzmed.ac.ir
Recruitment status
Recruitment complete
Funding source
Tabriz University of Medical Sciences, Iran Dairy Industries Company
Expected recruitment start date
2010-05-31, 1389/03/10
Expected recruitment end date
2010-07-12, 1389/04/21
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
The effects of consumption of probiotic yoghurt and conventional yoghurt on blood sugar, lipid profile, oxidative stress parameters and inflammatory marker in type 2 diabetic patients.
Public title
The effects of probiotic yoghurt on type 2 diabetes
Purpose
Supportive
Inclusion/Exclusion criteria
Inclusion criteria: having type 2 diabetes, 30 to 60 years old, LDL level more than 100 mg/Dl Exclusion criteria: having the renal or hepatic failure, cardiovascular disease, inflammatory bowel disease, thyroid disorders and lactose intolerance, treatment with insulin and taking corticosteroids, NSAIDS, immunosuppressives, cholesterol lowering drugs, multivitamin-mineral and fiber supplements, BMI more than 35 Kg/m2, smoking, pregnancy and lactation, use of multivitamin-mineral and fiber supplements 3 weeks prior to the study, use of antibiotics 1 month the study and during the study, having acute gastrointestinal disorders 1 month prior to the study and during the study
Age
From 30 years old to 60 years old
Gender
Both
Phase
N/A
Groups that have been masked
No information
Sample size
Target sample size: 60
Randomization (investigator's opinion)
Randomized
Randomization description
Blinding (investigator's opinion)
Double blinded
Blinding description
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Tabriz University of Medical Sciences
Street address
Golbad Ave
City
Tabriz
Postal code
Approval date
2010-05-31, 1389/03/10
Ethics committee reference number
897

Health conditions studied

1

Description of health condition studied
Type 2 Diabetes
ICD-10 code
E11
ICD-10 code description
Non-insulin-dependent diabetes mellitus

Primary outcomes

1

Description
hsCRP
Timepoint
baseline and after 6 weeks
Method of measurement
immunotorbidometry

2

Description
Fasting Blood Sugar
Timepoint
baseline and after 6 weeks
Method of measurement
spectrophotometry

3

Description
Hemoglobin A1c
Timepoint
baseline and after 6 weeks
Method of measurement
Chromatography

4

Description
Insulin
Timepoint
baseline and after 6 weeks
Method of measurement
ELIZA

5

Description
HOMA-IR
Timepoint
Baseline and after 6 weeks
Method of measurement
Formula

6

Description
Total Cholesterol
Timepoint
Baseline and after 6 weeks
Method of measurement
Spectrophotometry

7

Description
Triglyceride
Timepoint
Baseline and after 6 weeks
Method of measurement
Spectrophotometry

8

Description
HDL
Timepoint
Baseline and after 6 weeks
Method of measurement
Spectrophotometry

9

Description
LDL
Timepoint
Baseline and after 6 weeks
Method of measurement
Friedewald Formula

10

Description
Malondialdehyde
Timepoint
Baseline and after 6 weeks
Method of measurement
Spectrophotometry

11

Description
Catalase
Timepoint
Baseline and after 6 weeks
Method of measurement
Hugo Aebi Method

12

Description
Glutathione peroxidase
Timepoint
Baseline and after 6 weeks
Method of measurement
Spectrophotometry

13

Description
Superoxide dismutase
Timepoint
Baseline and after 6 weeks
Method of measurement
Spectrophotometry

14

Description
Total Antioxidant Capacity (TAC)
Timepoint
Baseline and after 6 weeks
Method of measurement
Spectrophotometry

Secondary outcomes

1

Description
Body Mass Index
Timepoint
Baseline and after 6 weeks
Method of measurement
Formula

2

Description
Dietary Factors
Timepoint
Baseline and after 6 weeks
Method of measurement
3-day 24h dietary recalls

3

Description
Blood Pressure
Timepoint
Baseline and after 6 weeks
Method of measurement
Sphygmomanometer

Intervention groups

1

Description
Intervention Group:Probiotic Yoghurt,300 gr/day,6 weeks
Category
Treatment - Drugs

2

Description
Control Group:Conventional Yoghurt,300 gr/day,6 weeks
Category
Placebo

Recruitment centers

1

Recruitment center
Name of recruitment center
Sina Endocrine Clinic
Full name of responsible person
Dr Javad Mohtadi Nia
Street address
City
Tabriz

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Tabriz University of Medical Sciences
Full name of responsible person
Dr Javad Mohtadi Nia
Street address
Golbad street
City
Tabriz
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Tabriz University of Medical Sciences
Proportion provided by this source
Public or private sector
empty
Domestic or foreign origin
empty
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
empty

2

Sponsor
Name of organization / entity
Iran Dairy Industries Company
Full name of responsible person
Ali Akbarian Moghari
Street address
Africa Boulevard
City
Tehran
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Iran Dairy Industries Company
Proportion provided by this source
Public or private sector
empty
Domestic or foreign origin
empty
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
empty

Person responsible for general inquiries

Contact
Name of organization / entity
Faculty of Health and Nutrition, Tabriz University of Medical Sciences
Full name of responsible person
Dr Javad Mohtadi Nia
Position
Associate Professor in Department of Food Science and Technology
Other areas of specialty/work
Street address
Attar Neyshabouri Street, Golgasht Street
City
Tabriz
Postal code
Phone
+98 41 1335 7580
Fax
Email
Mohtadiniaj@tbzmed.ac.ir,Haniejtahed@yahoo.com
Web page address

Person responsible for scientific inquiries

Contact
Name of organization / entity
Tabriz University of Medical Sciences
Full name of responsible person
Hanie Sadat Ejtahed
Position
MSc Student of Nutrition Science
Other areas of specialty/work
Street address
Attar Neyshabouri Street, Golgasht Street
City
Tabriz
Postal code
Phone
+98 41 1335 7580
Fax
Email
Mohtadiniaj@tbzmed.ac.ir,Haniejtahed@yahoo.com
Web page address

Person responsible for updating data

Contact
Name of organization / entity
Tabriz University of Medical Sciences
Full name of responsible person
Hanie Sadat Ejtahed
Position
MSc Student of Nutrition Science
Other areas of specialty/work
Street address
Attar Neyshabouri Street, Golgasht Street
City
Tabriz
Postal code
Phone
+98 41 1335 7580
Fax
Email
Mohtadiniaj@tbzmed.ac.ir,Haniejtahed@yahoo.com
Web page address

Sharing plan

Deidentified Individual Participant Data Set (IPD)
empty
Study Protocol
empty
Statistical Analysis Plan
empty
Informed Consent Form
empty
Clinical Study Report
empty
Analytic Code
empty
Data Dictionary
empty
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