Protocol summary

Summary
The aim of this study is to investigate the effects of resveratrol supplementation compared with calorie restriction diet on nutritional status, metabolic and oxidative parameters, serum sirtuin-1 and fibroblast growth factor-21 levels in patients with nonalcoholic fatty liver disease. Eighty-seven patients will randomly be divided in to 3 intervention groups including: resveratrol group (receive 2 capsules each containing 300 mg trans resveratrol); calorie restriction diet group (receive a prescribed diet for the purpose of losing maximum 10 percent of their initial body weight); placebo group: (receive 2 capsules each containing 300 mg starch) for 12 weeks. Researcher and patients are blinded in resveratrol and placebo groups. BMI, age and sex will be matched in 3 groups. All patients will receive basic education about principles of healthy eating. At the baseline and the end of the intervention, biochemical indices, anthropometric and blood pressure measurements will be assessed and compared between groups. Three-day food record questionnaires and physical activity questionnaire will be fulfilled at baseline, after 6 weeks and end of the intervention.

General information

Acronym
IRCT registration information
IRCT registration number: IRCT201511233664N16
Registration date: 2016-02-08, 1394/11/19
Registration timing: registered_while_recruiting

Last update:
Update count: 0
Registration date
2016-02-08, 1394/11/19
Registrant information
Name
Maryam Rafraf
Name of organization / entity
Tabriz University Of Medical Sciences
Country
Iran (Islamic Republic of)
Phone
+98 41 1335 7580
Email address
rafrafm@tbzmed.ac.ir
Recruitment status
Recruitment complete
Funding source
Nutrition Research Center, Vice-chancellor of Tabriz University of Medical Sciences.
Expected recruitment start date
2015-12-22, 1394/10/01
Expected recruitment end date
2016-06-04, 1395/03/15
Actual recruitment start date
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Actual recruitment end date
empty
Trial completion date
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Scientific title
Comparison of resveratrol supplementation, calorie restriction diet and placebo on nutritional status, metabolic and oxidative parameters, serum sirtuin-1 and fibroblast growth factor-21 levels in patients with nonalcoholic fatty liver disease
Public title
Comparison of resveratrol supplementation and calorie restriction diet effects on nutritional and metabolic status in patients with nonalcoholic fatty liver disease
Purpose
Supportive
Inclusion/Exclusion criteria
Inclusion criteria: age between 20 to 60 years from both gender; diagnosed with nonalcoholic fatty liver disease based on liver Ultrasonography; BMI=25-35; willingness to participate in the study. Exclusion criteria: pregnancy; breast feeding and postmenopausal women; professional athletes; smoking; alcohol consumption; following weight-reducing diet 3 months prior to the study; known liver disease (viral/etc.); inherited disorders affecting the liver; history of diagnosed cardiovascular, kidney, diabetes, gastrointestinal, pulmonary and autoimmune diseases, thyroid dysfunction and active cancer; recent surgery; use of medications such as corticosteroids, hepatotoxic drugs, hormonal drugs (such as oral contraceptive and/or estrogens), antidepressants and psychotropic medications, anticoagulant drugs, oral anti-diabetic and lipid-lowering drugs. Receiving any kinds of supplements 3 months prior to the study.
Age
From 18 years old to 60 years old
Gender
Both
Phase
2-3
Groups that have been masked
No information
Sample size
Target sample size: 87
Randomization (investigator's opinion)
Randomized
Randomization description
Blinding (investigator's opinion)
Double blinded
Blinding description
Placebo
Used
Assignment
Parallel
Other design features
This study is partially blinded. The intervention for resveratrol and placebo groups is indistinguishable for study subjects in those two groups. Randomization was carried out using a random allocation software (RAS).

Secondary Ids

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Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Tabriz University of Medical Sciences
Street address
Tabriz University of Medical Sciences, Golgasht street
City
Tabriz
Postal code
Approval date
2015-12-15, 1394/09/24
Ethics committee reference number
TBZMED.REC.1394.823

Health conditions studied

1

Description of health condition studied
nonalcoholic fatty liver disease
ICD-10 code
K76.0
ICD-10 code description
Fatty (change of) liver, not elsewhere classified

Primary outcomes

1

Description
liver enzymes (ALT, AST, GGT, ALP)
Timepoint
Baseline and after the intervention
Method of measurement
Spectrophotometry

2

Description
Liver echogenicity
Timepoint
Baseline and after the intervention
Method of measurement
Sonographic findings

3

Description
lipid profile (TC, TG, LDL-C, HDL-C, APO-A1, APO-B)
Timepoint
Baseline and after the intervention
Method of measurement
Biochemical

4

Description
Fasting blood glucose
Timepoint
Baseline and after the intervention
Method of measurement
Biochemical

5

Description
Insulin
Timepoint
Baseline and after the intervention
Method of measurement
ELISA

6

Description
Insulin resistance
Timepoint
Baseline and after the intervention
Method of measurement
HOMA-IR

7

Description
Oxidative status (TAC, MDA, GPx, SOD, Ox-LDL)
Timepoint
Baseline and after the intervention
Method of measurement
Biochemical

8

Description
Sirtuin-1
Timepoint
Baseline and after the intervention
Method of measurement
ELISA

9

Description
Fibroblast growth factor-21
Timepoint
Baseline and after the intervention
Method of measurement
ELISA

10

Description
Added at 2016-11-15: Plasminogen activator inhibitor-1 (PAI-1
Timepoint
Added at 2016-11-15: Baseline and after the intervention
Method of measurement
Added at 2016-11-15: ELISA

11

Description
Added at 2016-11-15: hs-CRP
Timepoint
Added at 2016-11-15: Baseline and after the intervention
Method of measurement
Added at 2016-11-15: Imonotorbidometry

Secondary outcomes

1

Description
systolic and diastolic blood pressure
Timepoint
Baseline and after the intervention
Method of measurement
Manometer

2

Description
Anthropometric measurements (BMI, WHR, WC)
Timepoint
Baseline and after the intervention
Method of measurement
Secca scale, non stretch meter

3

Description
Energy and nutrient intake
Timepoint
Baseline, end of week 6 and after the intervention
Method of measurement
3-day food record questionnaire

Intervention groups

1

Description
Intervention1: calorie restriction diet for 3 months
Category
Lifestyle

2

Description
Intervention 2: 2 capsules daily each contains 300 mg of trans resveratrol for 3 months
Category
Treatment - Drugs

3

Description
placebo: 2 capsules daily each contains 300 mg of starch for 3 months
Category
Placebo

Recruitment centers

1

Recruitment center
Name of recruitment center
Golgashti clinic
Full name of responsible person
Dr. Mohammad Hossein Somi
Street address
Azadi street
City
Tabriz

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Nutrition Research Center, vice-chancellor of Tabriz University of Medical Sciences
Full name of responsible person
Dr. Alireza Ostadrahimi
Street address
Faculty of Nutrition, Tabriz University of Medical Sciences, Golgasht street
City
Tabriz
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Nutrition Research Center, vice-chancellor of Tabriz University of Medical Sciences
Proportion provided by this source
100
Public or private sector
empty
Domestic or foreign origin
empty
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
empty

Person responsible for general inquiries

Contact
Name of organization / entity
Tabriz University of Medical Sciences
Full name of responsible person
Somayyeh Asghari
Position
Ph.D Candidate in Nutrition Sciences
Other areas of specialty/work
Street address
Nutrition Faculty, Tabriz University of Medical Sciences, Golgasht street
City
Tabriz
Postal code
Phone
+98 914 423 7085
Fax
Email
sasghari@tbzmed.ac.ir
Web page address

Person responsible for scientific inquiries

Contact
Name of organization / entity
Tabriz University of Medical Sciences
Full name of responsible person
Dr. Maryam Rafraf
Position
Ph.D in Nutrition Sciences
Other areas of specialty/work
Street address
Nutrition Faculty, Tabriz University of Medical Sciences, Golgasht street
City
Tabriz
Postal code
Phone
+98 41 3335 7580
Fax
Email
rafrafm@tbzmed.ac.ir
Web page address

Person responsible for updating data

Contact
Name of organization / entity
Tabriz University of Medical Sciences
Full name of responsible person
Somayyeh Asghari
Position
Ph.D Candidate in Nutrition Sciences
Other areas of specialty/work
Street address
Nutrition Faculty, Tabriz University of Medical Sciences, Golgasht street
City
Tabriz
Postal code
Phone
+98 41 3335 7581
Fax
Email
sasghari@tbzmed.ac.ir
Web page address

Sharing plan

Deidentified Individual Participant Data Set (IPD)
empty
Study Protocol
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Statistical Analysis Plan
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Informed Consent Form
empty
Clinical Study Report
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Analytic Code
empty
Data Dictionary
empty
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