-
Study aim
-
The aim of this study is to investigate the effect of high protein diet and standard protein on appetite status, body weight, body mass index (BMI), fat mass (FM) and lean body mass (LBM) in normal obese women.
-
Design
-
Design: In this randomized controlled trial, the participants randomly will be assigned to 2 groups. 25 women will be allocated in the control group to receive a diet regimen with a standard protein content (standard protein (SP); providing 0.8 g/kg body weight/day), and 25 women will be allocated in the intervention group to receive a diet regimen with a higher protein content (high protein (HP); providing 1.2 g/kg body weight/day) for 12 weeks. The diet regimens have an equal amount of calories in both groups according to the resting metabolic rate (BMR) based on the Harris-Benedict predictive formula.
-
Settings and conduct
-
The participants will not know whether they are a member of the HP group or the SP group.
-
Participants/Inclusion and exclusion criteria
-
Inclusion criteria into the study are for healthy women, aged between 30 to 60 years having a BMI (in kg/m2) <25 and >18.5, body fat>30 %. Exclusion criteria include: smoking, pregnancy, lactation, history or presence of kidney, gastrointestinal, liver, or thyroid disease, gout, certain cancers, or type 2 diabetes, severe weight changes within the last 3 months and bariatric surgery; recently following a high-protein diet or using anti-obesity medications or supplements; and consuming protein supplement and high amounts of caffeine (>250- 300 mg/d).
-
Intervention groups
-
high protein diet and standard protein
-
Main outcome variables
-
The weight, body composition including fat body mass and lean body mass by BIA machine and appetite level by visual analogue scale questionnaire will be measured before and after the trial.