Studying antibacterial and anti fungal properties by adding chitosan to complete denture tissue conditioners

Studying antibacterial and antifungal properties by adding chitosan to complete denture tissue conditioners

Individuals who need to be treated with tissue conditioners, randomly divided into seven groups. In the first group, patients receive the common tissue conditioners (control group) and in the second to seventh groups, patients have used tissue conditioners of Chitosan nanoparticles with a weight percent of 0.675, 1.25, 2.5 and 5, 10, and 20, respectively. Before the intervention and after 72 hours, the microbial culture is introduced from the mouth and into the denture samples by sterilized swabs for determined the rate of growth, proliferation, and activity of 4 microorganisms ( Enterococcus faecalis, Pseudomonas aeruginosa, Streptococcus mutans and Candida albicans).

Seven groups participated in the study. The first group patients received the common tissue conditioners (control ) and other 6 groups received a variety weight of Chitosan nanoparticles. None of the patients didn't inform about another Patient's materials. The person doing was also not aware of the type of material. Before the intervention and after 72 hours, the microbial culture is introduced from the mouth and into the denture samples by sterilized swabs for determined the rate of growth, proliferation, and activity of microorganisms.

Entrance criteria: Complete satisfaction to participate in the study-Patients with denture stomatitis Exit criteria: Drug and cigarette consumption-Sensitivity to the tested material

The first group patients received the common tissue conditioners (control ) and other 6 groups received Chitosan nanoparticles with a weight of 0.675, 1.25, 2.5 and 5, 10, and 20 respectively.

The amount of colonies produced by the use of Chitosan nanoparticles

The sampling method was randomized among individuals referring to the dental prosthodontics department of Tabriz Dental faculty. The randomization method is simple and the unit is the individual. Our tool to randomization is the randomized Number Chart. In this way, 42 people selected for study with numerical numbers were divided into seven groups. Common tissue Composer and materials with different weight percentages of Chitosan identified with codes A to G and sealed in envelopes that were placed in a Box and stirred.; Then they are randomly chosen from the Box and after observing the code, each material code was assigned to groups 1 to 7, respectively. None of the patients didn't inform about another Patient's materials. The person doing was also not aware of the type of material.

The current study is Double-blinded. Participants are unaware of the existence of several types of tissue conditioners in the study and are not aware of other participants. The person evaluating the growth of the bacterias is not aware of the type of group that provides a sample from it.