Efficacy of intrauterine infusion of platelet_rich plasma on pregnancy outcome in patients with repeated implantation failure.

To evaluate the effectiveness of platelet-rich plasma in improvement of pregnancy and implantation rate in women with history of repeated implantation failure.

Clinical trial with total of 120 eligible women that randomly assigned into two equal parallel groups (intervention and control)through Balanced Block Randomization. (Block size=4).

After endometrial preparation for embryo transfer in both groups, In intervention group,48 hours before embryo transfer (ET), 20 ml of peripheral venous blood will be obtained by syringe pre-filled with 2ml of anticoagulant solution,and then will be centrifuged immediately at 170 g for 10 minutes to separate red blood cells.this plasma will be re_centrifuged at 340 g for 5 minutes to obtain platelet-rich plasma (PRP), that contained platelet 4-5 times more than peripheral blood. 0.5ml of PRP will be infused into the uterine cavity with IUI catheter and after 48 hours, ET will be done. In control group, ET will be done without intrauterine infusion of PRP. After 2 weeks of ET, serum Beta_human chorionic Gonadotropin ( β HCG) will be measured in all patients. This research will be done at Infertility Department Of Shariati Hospital, Tehran, Iran

Infertile 20-40 years old women that failed to conceive after three or more embryo transfer and have at least one frozen good - quality blastocyst- stage embryo,body mass index below 30 kg/m2, without Genetic, Chromosomal, Uterine, Immunologic, Hormonal and Hematologic disorders.

In intervention group,48 hours before embryo transfer, 0.5ml of platelet-rich plasma will be infused into the uterine cavity with IUI catheter. In control group embryo transfer undergoing without intrauterine infusion of platelet-rich plasma.

Clinical Pregnancy Implantation Rate

Balanced Block Randomization (Block size=4): We prepare four balls,two A and two B.for first patient ,a nurse who does not know our plan for both A and B groups, selects one ball randomly from basket.for next patient this selection were done through remaining three balls.for third patient from remaining two balls and the last ball for the fourth patient, and then another block was selected.therefore our blocks show one of these pattern. (BAAB) (BABA) (ABBA) (BBAA) (ABAB) (AABB)