Protocol summary

Summary
To study the effects of synbiotics supplement on lipid profile, liver enzymes, inflammatory factors and hepatic fibrosis in patients with Nonalcoholic Steatohepatitis (NASH), 50 patients who referred to Gastrointestinal (GI) clinic with steatosis grade 1 or more will be randomly allocated to receive 2 protexin capsules or placebos for 7 months; both groups will be advised to adherence our diet and exercise program too. At the first and the end of the intervention, lipid profiles, liver enzymes, some inflammatory cytokines, and liver fibrosis will be assessed and compared between groups.

General information

Acronym
IRCT registration information
IRCT registration number: IRCT201012084010N4
Registration date: 2012-11-30, 1391/09/10
Registration timing: retrospective

Last update:
Update count: 0
Registration date
2012-11-30, 1391/09/10
Registrant information
Name
Azita Hekmatdoost
Name of organization / entity
Shahid Beheshti University of Medical Sciences, National Institute of Nutrition Research
Country
Iran (Islamic Republic of)
Phone
+98 21 2293 0824
Email address
hekmat@sina.tums.ac.ir
Recruitment status
Recruitment complete
Funding source
National Nutrition and Food Technology Research Institute (NNFTRI) and Digestive Disease Research Institute (DDRI)
Expected recruitment start date
2012-02-22, 1390/12/03
Expected recruitment end date
2012-09-25, 1391/07/04
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
The effects of synbiotics supplement on lipid profile, liver enzymes, inflammatory factors and hepatic fibrosis in patients with Nonalcoholic Steatohepatitis
Public title
The effects of healthy bacteria supplementation on lipid profile, liver enzymes, inflammatory factors and hepatic fibrosis in patients with Nonalcoholic Steatohepatitis
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria: Age of 18 years and older; BMI between 25-40; Abnormal serum transferase (ALT & AST more than 1.5 fold of normal range); Sonographic findings compatible with hepatic steatosis (degree 2 or more). Exclusion criteria: Diabetes; Using antibiotic 2 weeks before recruitment to the study; A history of alcohol consumption; Pregnancy & Breast feeding, Professional athletes; Liver disease (viral/etc); Use of drugs such as calcium channel blockers, high dose synthetic estrogens, methotrexate , amiodarone, steroids, chloroquine, immunosuppressive drugs, lipid-lowering agents, metformin and vitamin E; A history of Hypertension, Cardiovascular disease, Pulmonary disease, Renal disease & Celiac disease; Cirrhosis; Medications causing NAS; Upper GI surgery / Prior surgical procedures such as jejunoileal or jejunocolic bypass, gastroplasty, following program to lose weight in recent 3 mo;-A history of hypothyroidism or Cushing’s syndrome.
Age
From 18 years old to 70 years old
Gender
Both
Phase
2-3
Groups that have been masked
No information
Sample size
Target sample size: 50
Randomization (investigator's opinion)
Randomized
Randomization description
Blinding (investigator's opinion)
Triple blinded
Blinding description
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

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Ethics committees

1

Ethics committee
Name of ethics committee
National Nutrition and Food Technology Research Institute (NNFTRI)
Street address
#7, Shahid Hafezi Ave, Farahzadi Blvd, Shahrak Gharb
City
Tehran
Postal code
1981619573
Approval date
2010-08-20, 1389/05/29
Ethics committee reference number
046466

Health conditions studied

1

Description of health condition studied
Nonalcoholic steatohepatitis (NASH)
ICD-10 code
K75.8
ICD-10 code description
Nonalcoholic steatohepatitis (NASH)

Primary outcomes

1

Description
ALT, AST, GGT
Timepoint
at the beginning and at the last
Method of measurement
Biochemistry

2

Description
hs-CRP, TNF-a
Timepoint
at the beginning and at the last
Method of measurement
ELISA & Nephlometer

3

Description
liver fibrosis
Timepoint
at the beginning and at the last
Method of measurement
Fibroscan

4

Description
FBS
Timepoint
at the beginning and at the last
Method of measurement
Biochemistry

5

Description
cholestrol, TG, LDL-c, HDL-c
Timepoint
at the beginning and at the last
Method of measurement
Biochemistry

Secondary outcomes

1

Description
weight and BMI
Timepoint
at weeks: 0, 14, 28
Method of measurement
Weight will be measured by Secca scale , BMI by formula

2

Description
waist circumference, hip circumfereance
Timepoint
at weeks: 0, 14, 28
Method of measurement
non stretch meter

3

Description
insulin
Timepoint
at weeks: 0, 14, 28
Method of measurement
ELISA

4

Description
insulin resistance
Timepoint
at weeks: 0, 28
Method of measurement
formula

Intervention groups

1

Description
intervention: 2 protexin capsules for 7 months
Category
Treatment - Drugs

2

Description
control: 2 placebo capsule (Maltodexterin) for 7 months
Category
Placebo

Recruitment centers

1

Recruitment center
Name of recruitment center
GI clinic
Full name of responsible person
Street address
City
Tehran

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
National Nutrition and Food Technology Research Institute (NNFTRI)
Full name of responsible person
Dr Hajifaraji
Street address
#7, West Arghavan, Farahzadi BLV, Shahrak Gharb
City
Tehran
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
National Nutrition and Food Technology Research Institute (NNFTRI)
Proportion provided by this source
100
Public or private sector
empty
Domestic or foreign origin
empty
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
empty

Person responsible for general inquiries

Contact
Name of organization / entity
National Nutrition and Food Technology Research Institute (NNFTRI)
Full name of responsible person
Dr Azita Hekmatdoost
Position
MD, PhD/ Assistant Prof
Other areas of specialty/work
Street address
#7, West arghavan, Farahzadi Blv, Shahrak Gharb
City
Tehran
Postal code
Phone
+98 21 2235 7484
Fax
Email
a_hekmat2000@yahoo.com
Web page address

Person responsible for scientific inquiries

Contact
Name of organization / entity
National Nutrition and Food Technology Research Institute (NNFTRI)
Full name of responsible person
Dr Azita Hekmatdoost
Position
MD, PhD/ Assistant Prof
Other areas of specialty/work
Street address
#7, West Arghavan, Farahzadi BLv, Shahrak Gharb
City
Tehran
Postal code
Phone
+98 21 2235 7484
Fax
Email
a_hekmat2000@yahoo.com
Web page address

Person responsible for updating data

Contact

Sharing plan

Deidentified Individual Participant Data Set (IPD)
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Study Protocol
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Statistical Analysis Plan
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Informed Consent Form
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Clinical Study Report
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Analytic Code
empty
Data Dictionary
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