Effects of Pomegranate juice consumption on serum vascular and systematic inflammation factors, advanced glycation end products, NF-kB and Sirtuin1 activity in mononuclear cells in type2 diabetic patients

Cardiovascular diseases, nephropathy, blindness and amputation are the main complications of diabetes mellitus. These complications are mainly because of micro and macro angiopathy. High serum concentrations of oxidative stress, inflammation markers and advanced glycated end products are important risk factors for the development of diabetic complications. Pomegranate juice has antioxidant and anti- inflammatory properties. The purpose of this study is to determine the effects of pomegranate juice consumption on serum vascular and systematic inflammation factors, advanced glycation end products, NF-kB and Sirtuin1 activity in mononuclear cells in type2 diabetic patients. This study is a randomized double blind clinical trial performs on 48, 40-65 years old diabetic patients recruit from “Special Diabetic Affairs Foundation”. The patients are randomly allocated either to pomegranate juice consumption group or control group. PJ and control group will ask to consume 240 ml/day of PJ (without added sugar or additives) or a control beverage of similar color and energy and glucose content for 3 months. The questionnaires of general characters and three days 24-hour food recall will be completed by interview. Anthropometric measurements will be measured and 20 ml blood collected from each patient after 12-14h overnight fasting, at baseline and at the end of the study. Data will be analyzed using SPSS software. Independent-T-test will be used to compare pre and post intervention variables between groups. Covariance analysis will be used to compare blood parameters of two groups at the end of the study, adjusted for baseline measures.

Institute of Nutrition And Food Sciences ; Research Institute for Endocrine Sciences