Protocol summary

Summary
Objectives: improvement of left ventricular function in STEMI patients after intramyocardial injections of 2 types of autologous bone marrow derived cells into the infarcted myocardium compared to a placebo group during CABG. Design: A Phase II/III, Multicenter, Placebo-Controlled, Randomized, Double-Blind Clinical Trial Methods: 90 patients with first anterior STEMI (10 days - 3 months), 18-75 years, both gender and CABG candidate selected for randomization in 3 groups [CD133+, the MNC (MonoNuclear Cell), and placebo] and intramyocardial injection during CABG. They underwent real or sham aspiration. They followed at 6 and 18 months after surgery by SPECT and clinical assessments. The primary endpoint was assessed by changes in global left ventricular ejection fraction (LVEF) at rest by gated SPECT.

General information

Acronym
IRCT registration information
IRCT registration number: IRCT201612201031N20
Registration date: 2017-01-14, 1395/10/25
Registration timing: retrospective

Last update:
Update count: 0
Registration date
2017-01-14, 1395/10/25
Registrant information
Name
Nasser Aghdami
Name of organization / entity
Royan Institute
Country
Iran (Islamic Republic of)
Phone
+98 21 2356 2000
Email address
nasser.aghdami@royaninstitute.org
Recruitment status
Recruitment complete
Funding source
Department of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology
Expected recruitment start date
2007-12-31, 1386/10/10
Expected recruitment end date
2012-06-30, 1391/04/10
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
The effect of intramyocardial transplantation of autologous bone marrow derived MNC (MonoNuclear Cell) and CD133+ cells comparison to placebo, on LVEF in MI patients, A Phase II/III, Multicenter, Placebo-Controlled, Randomized, Double-Blind Clinical Trial
Public title
Intramyocardial injection of transplantation of autologous bone marrow derived MNC (MonoNuclear Cell) and CD133+ cells comparison to placebo, on LVEF in MI patients
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria: 18-75 years; First ST elevation myocardial infarction (STEMI) 10 days - 3 months; Post-acute myocardial infarction (AMI) left ventricular ejection fraction (LVEF) as assessed by echocardiography: 20%-45%; Target lesion must be located in the left anterior descending (LAD) section; Coronary artery bypass graft (CABG) candidate; At least 4 akinetic segments (assessed by stress echocardiography). Exclusion criteria: History of prior AMI; Active infection; History of recurrent infection; Positive test for syphilis (RPR); Hepatitis B and C (HBsAg, anti-HBc, anti-HCV); HIV or HTLV-1; Documented terminal illness or malignancy; Documented autoimmune disease; Pulmonary edema; Urgent or emergency CABG; Systolic blood pressure <80 mmHg; 4000 > leukocytes (µL) >15000; AST >100 IU/L or ALT >100 IU/L; Hemoglobin <10 g/dL; Platelets <100,000/µL; INR >1.8
Age
From 18 years old to 75 years old
Gender
Both
Phase
2-3
Groups that have been masked
No information
Sample size
Target sample size: 90
Randomization (investigator's opinion)
Randomized
Randomization description
Blinding (investigator's opinion)
Double blinded
Blinding description
Placebo
Used
Assignment
Parallel
Other design features
Computer-generated randomization sequence in a 1:1:1 ratio between the CD133+, the MNC, and placebo groups

Secondary Ids

1

Registry name
ClinicalTrials.gov
Secondary trial Id
NCT01167751
Registration date
2010-07-19, 1389/04/28

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Royan infertility institute
Street address
Royan institute, Eastern Hafez St,Northern Banihashem St, 45 m Resalat highway
City
Tehran
Postal code
Approval date
2006-10-04, 1385/07/12
Ethics committee reference number
پ-85-106

Health conditions studied

1

Description of health condition studied
Acute transmural myocardial infarction of anterior wall
ICD-10 code
I21.0
ICD-10 code description
Transmural infarction (acute)(of): • anterior (wall) NOS • anteroapical • anterolateral • anteroseptal

Primary outcomes

1

Description
Changes in global left ventricular ejection fraction (LVEF) at rest between group
Timepoint
Base, 6 and 18 months after CABG
Method of measurement
SPECT

Secondary outcomes

1

Description
NYHA classification
Timepoint
base and 18 month after CABG
Method of measurement
Standard classification of NYHA

2

Description
Perfusion defect score (PDS)
Timepoint
Base, 6 and 18 months after CABG
Method of measurement
SPECT

3

Description
Non-viable (NV) segments
Timepoint
Base, 6 and 18 months after CABG
Method of measurement
SPECT

4

Description
Decrease systolic wall thickening (Dec. Thickening) of the myocardium
Timepoint
Base, 6 and 18 months after CABG
Method of measurement
SPECT

5

Description
Wall motion score (WMS)
Timepoint
Base, 6 and 18 months after CABG
Method of measurement
SPECT

6

Description
Adverse events assessment (included death, reinfarction, implantable cardioverter defibrillator (ICD) placement, infection, and arrhythmia)
Timepoint
Base, 6 and 18 months after CABG
Method of measurement
Physician assessment

Intervention groups

1

Description
Intervention: Intramyocardial injection of 3 cc authologous bone marrow derived cell (MNC) using a 22-gauge needle
Category
Treatment - Other

2

Description
Intervention: Intramyocardial injection of 3 cc authologous bone marrow derived cell (CD133) using a 22-gauge needle
Category
Treatment - Other

3

Description
Intervention: Intramyocardial injection of 3 cc placebo using a 22-gauge needle
Category
Placebo

Recruitment centers

1

Recruitment center
Name of recruitment center
Baqiyatallah Hospital
Full name of responsible person
Dr. Mohammad Hassan Naseri
Street address
Molasadra St., Chamran Highway
City
Tehran

2

Recruitment center
Name of recruitment center
Shahid Dr. Lavasani Hospital
Full name of responsible person
Dr. Hossein Hosseinnejad
Street address
Tehransar
City
Tehran

3

Recruitment center
Name of recruitment center
Tehran Heart Center Hospital
Full name of responsible person
Dr.Hossein Ahmadi
Street address
North Karegar St.
City
Tehran

4

Recruitment center
Name of recruitment center
Rajaie Cardiovascular Medical and Research Center
Full name of responsible person
Dr.Saeed Hosseini
Street address
Niyayesh ave crossroad, valiasr st.
City
Tehran

5

Recruitment center
Name of recruitment center
Masih Daneshvari Hospital
Full name of responsible person
Dr.Zargham Ahmadi
Street address
Darabad, Bahonar St.
City
Tehran

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Royan Institute
Full name of responsible person
Hamid Gourabi
Street address
Eastern Hafez St,Northern Banihashem St, 45 m Resalat highway
City
Tehran
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Royan Institute
Proportion provided by this source
100
Public or private sector
empty
Domestic or foreign origin
empty
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
empty

Person responsible for general inquiries

Contact
Name of organization / entity
Royan Institute
Full name of responsible person
Hoda Madani
Position
M.D/ Researcher
Other areas of specialty/work
Street address
Eastern Hafez St, Northern Banihashem St, 45 m Resalat highway
City
Tehran
Postal code
Phone
+98 21 2356 2458
Fax
Email
hoda62_m@yahoo.com
Web page address

Person responsible for scientific inquiries

Contact
Name of organization / entity
Royan Institute
Full name of responsible person
Nasser Aghdami
Position
M.D/Ph.D
Other areas of specialty/work
Street address
Eastern Hafez St,Northern Banihashem St, 45 m Resalat highway
City
Tehran
Postal code
Phone
+98 21 2356 2000
Fax
Email
nasser.aghdami@royaninstitute.org
Web page address

Person responsible for updating data

Contact
Name of organization / entity
Royan Institute
Full name of responsible person
Hoda Madani
Position
Researcher/M.D
Other areas of specialty/work
Street address
Eastern Hafez St,Northern Banihashem St, 45 m Resalat highway
City
Tehran
Postal code
Phone
+98 21 2356 2458
Fax
Email
hoda62_m@yahoo.com
Web page address

Sharing plan

Deidentified Individual Participant Data Set (IPD)
empty
Study Protocol
empty
Statistical Analysis Plan
empty
Informed Consent Form
empty
Clinical Study Report
empty
Analytic Code
empty
Data Dictionary
empty
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