Comparison of the effect of dietary approaches to stop hypertension (DASH) diet with regular hypocaloric diet on cardiometabolic parameters, oxidative stress and inflammatory markers, hepatic enzymes, and fatty liver severity in patients with non-alcoholic fatty liver disease

Comparison of the effect of dietary approaches to stop hypertension (DASH) diet with regular hypocaloric diet on cardiometabolic parameters, oxidative stress and inflammatory markers, hepatic enzymes, and fatty liver severity in patients with non-alcoholic fatty liver disease

The clinical trial has a control group, with 3 parallel groups (40 in each group), single blinded, randomized by random number table method

The number of 120 eligible patients for the study (40 in each group) will be selected.Then the subjects will be divided into three groups by permuted block randomization.The three groups include: dietary approaches to stop hypertension (DASH) diet, Hypocaloric diet, and DASH + Hypocaloric diet. The diet will be written to each individual. In this way, the required energy will be calculated based on Harris Benedict formula, In Group 1 the required energy will be determined and without deduction of energy units of each food groups will be determined based on the DASH diet. In Group 2 and 3 the rate of 500 kcal will be deducted, then units of each food groups will be determined based on a regular diet in group 2 and DASH diet in group 3. After 3 months of intervention, all groups will be compared for their changes in cardiometabolic parameters, oxidative stress, inflammatory markers, hepatic enzymes, and fatty liver severity.

Inclusion criteria: NAFLD patients should have BMI more than 25 exclusion criteria: Patients with dire diseases, Pregnancy, Alcohol consumption

The subjects divide into 3 groups, Group 1: receiving dietary approaches to stop hypertension (DASH) diet, Group 2: receiving hypocaloric diet, Group 3: receiving hypocaloric DASH diet

Cardiometabolic parameters; Oxidative stress and inflammatory markers; Hepatic enzymes; Fatty liver severity

Permuted block randomization: First, the list of participants was provided according to their referral. Then all possible sequences that can be written with three groups, were written on paper (for 3 groups 6 sequences can be written). Then one of the numbers from 1 to 6 was considered for each sequence, for example for the ABC sequence 1 and for the ACB sequence 2 and as such, one of the numbers 3 to 6 was considered for the other 4 sequences. Then, using random number table, the random numbers were selected from one point to the left or right. Wherever the random number was 1 to 6, the corresponding sequence was recorded on the paper, and wherever the numbers were 0 or 7, 8, 9, the next number that was between 1 to 6 was considered. This method continued until groups for the total number of participants were determined. Then, the names of subjects on the list were allocated to the specific groups according to the sequence of groups which had been determined by the above mentioned procedure.

Participants are unaware of the differences in the incoming regimes.