Effects of 4 months of 25 Hydroxy vitamin D3 supplementation and probiotics independently and concomitantly on some factors affecting cancer progression in patients with breast cancer undergoing surgery referred to Shahid Bahonar Hospital in Kerman.
The effects of probiotic and 25-hydroxy vitamin D3 consumption in breast cancer patients.
Design
A double-blind clinical trial (patient and physician) with four intervention groups randomized by the factorial method.
Settings and conduct
The trial will be performed in the hematology and oncology ward of Shahid Bahonar Hospital in Kerman. Patients who meet the inclusion criteria will be randomly assigned to one of four intervention groups. The physician and patient are blinded, and patients will receive the intervention for four months, and the primary and secondary outcomes will be assessed at the beginning and the end of the study.
Participants/Inclusion and exclusion criteria
Women over 18 years old who have Breast Cancer Stage III, II (luminal A, luminal B, Her2 ENRICHED) are approved histopathologically and treated with neoadjuvant and surgical treatment.
Intervention groups
Intervention group 1: One daily capsule of 25 hydroxy vitamin D 3 1000 IU daily with probiotic placebo capsule for 4 months
Intervention group 2: One probiotic capsule daily, plus one vitamin D placebo capsule for 4 months.
Intervention group 3: One probiotic capsule daily 1000000000 CFU plus one 25 hydroxy vitamin D 3 1000 IU capsule for 4 months.
Intervention group 4: one probiotic placebo capsule daily and one vitamin D3 placebo capsule for 4 months.
Effects of 4 months of 25 Hydroxy vitamin D3 supplementation and probiotics independently and concomitantly on some factors affecting cancer progression in patients with breast cancer undergoing surgery referred to Shahid Bahonar Hospital in Kerman.
Public title
Vitamin D, Probiotics and Breast Cancer
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Women over 18 years with clinical stage II and III breast cancer diagnosis.
Undergoing Neo-adjuvant Treatment and Surgery.
Exclusion criteria:
Age less than 18 years
Stages I and IV of Breast Cancer
BMI >30
Non-surgical advanced or metastatic cancers
Cancer with a Basal-like Molecular Pattern
Patients who have been taking vitamin D or probiotic supplements for the past three months.
People with hyperparathyroidism or hypoparathyroidism
active Kidney stones in the past six months
Renal Failure (Creatinine> 190mmol / L)
Hypercalcemia
History of taking part in other intervention tests.
Hormone therapy history.
History of radiotherapy
Recent use of estrogenic drugs such as birth control pills
Chronic diarrhea or constipation (over 4 consecutive weeks)
History of inflammatory bowel disease
Celiac disease, chronic pancreatitis, history of colectomy and ileostomy
History of gallstones
Liver Failures
Known cases of AIDS
Continuous and long-term use of antibiotics, consumption of aluminum hydroxate
History of Thiazide diuretics
Age
From 18 years old
Gender
Female
Phase
3
Groups that have been masked
Participant
Care provider
Investigator
Sample size
Target sample size:
88
Randomization (investigator's opinion)
Randomized
Randomization description
Patients will be divided into four intervention groups by the factorial method.
Randomization Method: Factorial
Randomization Unit: Person
Randomization Tool: Random Number Table
Blinding (investigator's opinion)
Double blinded
Blinding description
People will be kept blind.
1. Patients: Each patient will receive a label according to the random number table that will appear on the patient's drug box. Medicines do not differ in appearance, taste, odor. The patient consumes two capsules daily.
2. Physician: The analyzing team will give the medicine boxes to the physician, and the physician will provide the medication based on the random number table.
Placebo
Used
Assignment
Factorial
Other design features
Secondary Ids
empty
Ethics committees
1
Ethics committee
Name of ethics committee
Research Ethics Committee of Kerman University of Medical Sciences
Street address
Beginning of Ibn Sina Street, First of Jihad Boulevard, Somayeh intersection (Tahmasb Abad)
City
Kerman
Province
Kerman
Postal code
7616913555
Approval date
2020-03-10, 1398/12/20
Ethics committee reference number
IR.KMU.REC.1398.709
Health conditions studied
1
Description of health condition studied
Breast Cancer
ICD-10 code
ICD-10 code description
Primary outcomes
1
Description
Measurement of Miller-Payne Grade Index on Residual Tumor Tissue
Timepoint
4 months after intervention
Method of measurement
Microscopic examination
2
Description
Residual Cancer Burden Score on residual tumor tissue
Timepoint
4 months after intervention
Method of measurement
By http://www.mdanderson.org/breastcancer_RCB.
3
Description
Ki-67 nuclear protein as a biomarker of cell proliferation
Timepoint
َAt the beginning of the intervention, by Core needle biopsy and 4 months later (after surgery) on the residual tumor tissue
Method of measurement
Immunohistochemical staining
Secondary outcomes
1
Description
Serum IL-1 beta
Timepoint
On the first day of the intervention and 4 months after the intervention
Method of measurement
enzyme-linked immunosorbent assay(ٍELISA)
2
Description
Serum IL-6
Timepoint
On the first day of the intervention and 4 months after the intervention
Method of measurement
ELISA
3
Description
Serum IL-10
Timepoint
On the first day of the intervention and 4 months after the intervention
Method of measurement
ELISA
4
Description
Serum TNF alpha
Timepoint
On the first day of the intervention and 4 months after the intervention
Method of measurement
ELISA
Intervention groups
1
Description
Intervention group: Patients receiving vitamin D3 at a dose of 1000 IU daily as a single oral capsule, with food, and with a probiotic placebo capsule for 16 consecutive weeks from breast cancer diagnosis until surgery.
Category
Treatment - Drugs
2
Description
Intervention group: Patients who receive single probiotics capsule daily at 1 billion CFU before meals with a single vitamin D3 placebo capsule for at least 16 weeks from cancer diagnosis until surgery
Category
Treatment - Drugs
3
Description
Intervention group: Patients receiving vitamin D3 at a dose of 1000 IU daily as a capsule, with food and probiotic capsules daily with 1 billion CFU for 16 consecutive weeks from breast cancer diagnosis until surgery.
Category
Treatment - Drugs
4
Description
Intervention group: Patients who receive one vitamin D3 placebo capsule with food and one probiotic placebo capsule daily before meals for 16 consecutive weeks from breast cancer diagnosis until surgery
Category
Placebo
Recruitment centers
1
Recruitment center
Name of recruitment center
Shahid Bahonar Hospital
Full name of responsible person
Ali Asghar Tirgar
Street address
Kerman Province, Kerman, Qaraney St
City
Kerman
Province
Kerman
Postal code
7616913555
Phone
+98 34 3223 5011
Fax
+98 34 3226 0100
Email
tirgar4@gmail.com
Web page address
http://bh.kmu.ac.ir/fa
Sponsors / Funding sources
1
Sponsor
Name of organization / entity
Kerman University of Medical Sciences
Full name of responsible person
Dr. Abbas Pardakhty
Street address
Kerman University of Medical Sciences,Medical University Campus,Haft-Bagh Highway, Kerman, Iran
City
Kerman
Province
Kerman
Postal code
7616913555
Phone
+98 34 3132 5856
Email
abpardakhty@kmu.ac.ir
Web page address
http://kmu.ac.ir/fa
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Is the source of funding the same sponsor organization/entity?
No
Title of funding source
Zist Takhmir Pharmaceutical Company
Proportion provided by this source
25
Public or private sector
Private
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Industry
Person responsible for general inquiries
Contact
Name of organization / entity
Kerman University of Medical Sciences
Full name of responsible person
Ali Asghar Tirgar
Position
Medical Student
Latest degree
Medical doctor
Other areas of specialty/work
General Practitioner
Street address
Shahid Bahonar Hospital, Qaraney Street, Kerman
City
Kerman
Province
Kerman
Postal code
7616913555
Phone
+98 34 1223 5019
Email
tirgar4@gmail.com
Person responsible for scientific inquiries
Contact
Name of organization / entity
Kerman University of Medical Sciences
Full name of responsible person
Masoud Rezaei
Position
Medical Student
Latest degree
Medical doctor
Other areas of specialty/work
General Practitioner
Street address
َAlley 3, Shahab Street, Qaraney square
City
Kerman
Province
Kerman
Postal code
7616886988
Phone
+98 34 3222 1558
Email
masoudrezaei082@gmail.com
Person responsible for updating data
Contact
Name of organization / entity
Kerman University of Medical Sciences
Full name of responsible person
Masoud Rezaei
Position
Medical Student
Latest degree
Medical doctor
Other areas of specialty/work
General Practitioner
Street address
َAlley 3, Shahab Street, Qaraney square
City
Kerman
Province
Kerman
Postal code
7616886988
Phone
+98 34 3222 1558
Email
masoudrezaei082@gmail.com
Sharing plan
Deidentified Individual Participant Data Set (IPD)
Yes - There is a plan to make this available
Study Protocol
Yes - There is a plan to make this available
Statistical Analysis Plan
Yes - There is a plan to make this available
Informed Consent Form
Yes - There is a plan to make this available
Clinical Study Report
Yes - There is a plan to make this available
Analytic Code
Not applicable
Data Dictionary
Yes - There is a plan to make this available
Title and more details about the data/document
All data will be initially de-identified.
1. Demographic Data of Participants: These data will generally be included in a table in the article.
2. Main outcome data: The article will be appended to the article in charts and tables as well as in an Excel file.
Secondary outcome data in the article will be attached to the article in graphs and tables as well as in an Excel file.
When the data will become available and for how long
Starting data access period six months after the results are published
To whom data/document is available
Data will be available only to researchers working in academic and scientific institutions approved by the Ministry of Health
Under which criteria data/document could be used
The data will be available to researchers for further statistical analysis
From where data/document is obtainable
Refer to the person responsible for the project's scientific accountability to access the data
What processes are involved for a request to access data/document
Applicants will submit a personal resume and the purpose of the data to the person responsible for the scientific accountability of the plan. The responsible person will be required to investigate the request within one week of sending the researcher information.