Growth hormone, insulin-like growth factor 1, testosterone and cortisol responses to single bout of agility ladder exercise in water polo athletes

Investigating the acute response of growth hormone, testosterone, insulin-like growth factor-1, cortisol and testosterone/cortisol ratio following an agility ladder exercise session in male water polo athletes.

Single-blind, parallel-group, randomized controlled trial

The participants received their intervention based on the group name. The training place was Azadi Complex in Tehran. Blood samples were taken from all participants before and after the exercise, as well as 24 and 48 hours after the exercise. At the beginning of the research, a code was assigned to each subject and in all subsequent steps, the code was used instead of their name.

Inclusion criteria included water polo male athletes in Tehran Azadi Sport Complex, 19-22 year old. The exclusion criteria including having blood disease, infection, allergic conditions, and use of drug, supplements or tobacco.

Control group: The participants in this group warmed up at the beginning of the agility ladder exercise session, did not exercise during the session, and did cool down at the end of the session. Experimental group: This group participant will perform an agility ladder exercise session after warm up (about 40 minutes) and then performed cooling down.

Growth hormone, insulin-like growth factor 1, testosterone and cortisol

Computerization was performed using a computer and a simple randomization method. The method used by the computer was that the computer selects random numbers between 0.5 and 1. Numbers less than 0.5 were in the control group and numbers greater than 0.5 were in the intervention group. Therefore, 30 subjects were randomly divided into two groups.

The participants were identified using the codes instead of their actual names. A third party was asked to classify the participants randomly, using the (labelled) codes. Study groups had codes instead of names. The laboratory was uninformed from name of groups and only used the codes. Groups codes were used for statistical analysis.

The data of the dependent research variables can be published as code and without name of the participants.

They will be available after article submission.

Researchers

Data is available for re-analysis by researchers

Corresponding author. Dr. Saleh Rahmati-Ahmadabad. Department of Physical Education, Pardis Branch, Islamic Azad University, Pardis, Iran

Send the request by email to the corresponding author.