The effect of aerobic physical activity alone and in combination with hemp seed powder consumption on biophysical, metabolic and neurological parameters in sedentary young men

Determining the effect of aerobic physical activity alone with hemp seed powder consumption on biophysical, metabolic and neurological factors in sedentary young men

Randomized, double-blind clinical trial with four parallel groups

The study will be performed in the ergometrics laboratory of the Faculty of Health and Nutrition and in clinics affiliated to Tabriz University of Medical Sciences and the duration of the supplementary course will be 8 weeks. hemp seed powder and placebo capsules will be coded by the person in charge of preparation and the main researchers and patients will be blind to the type of supplement taken in each group.

Use any medication or diet and exercise supplement for at least 2 months before the start of the study, respiratory disease, cardiovascular disease and hypertension, diabetes, thyroid disease, neurological disease, musculoskeletal disease, autoimmune disease, skeletal and joint problems

The intervention group will consume 2 capsules of hemp powder daily and will also work out on the treadmill three times a week. The control group will also take a capsule containing cornstarch (placebo) which will be similar to the supplement.

Biophysical factors (balance, strength, mental function), Brain derived neuronal factor (BDNF), Neuropeptide Y (NPY), Blood insulin and insulin resistance, Fasting blood sugar, Lipid profile, Renal function indicators (total iron-binding capacity, creatinine, urea, uric acid), Serum electrolytes (total iron-binding capacity, magnesium, iron, calcium, phosphorus), high-sensitivity C-reactive protein, Liver function (ALT, AST, direct bilirubin, ALP, albumin, total bilirubin, total protein), Lactate dehydrogenase activity, Creatine kinase activity, testosterone, SHBG, oxidative stress status (MDA، SOD، GPX،TAC, Catalase)

In this study, we will use block randomization. Blocking is usually used to balance the number of samples assigned to each of the study groups. This feature helps researchers to equate the number of samples assigned to each of the study groups in cases where intermediate analyzes are required during the sampling process. The size of all the blocks is the same and we will have 4 blocks in this four-group experiment. The randomization tool is also used from the site www.sealedenvelope.com, which is a site for generating random sequences, in addition to simple randomization, is able to generate random sequences by blocking method. For concealment, we use allocation concealment, which is the method used to execute a random sequence on study participants, so that the assigned group is not known before the individual is assigned. Using opaque envelopes sealed with a random sequence (Sequentially numbered, sealed, opaque envelopes) in which in this method each random sequence is recorded on a card and the cards are placed in the envelopes respectively. Are placed. In order to maintain a random sequence, the envelopes are numbered in the same way on the outer surface. Finally, the lids of the letter envelopes are glued and placed inside a box, respectively. At the beginning of the registration of participants, based on the order of entry of eligible participants into the study, one of the envelopes of the letter is opened in order and the assigned group of the participant is revealed.

In this study, the main researchers (including students, supervisors, and advisors) as well as participants will be unaware of the type of supplement (hemp seed or placebo) received by each group. The person in charge of preparing the supplement packs (who is completely unrelated to the study) will be asked to assign a three-digit code to each of the two powders (hemp seed and placebo) and, until the end of the study and data analysis, Keep the codes with you.