Evaluation of the effectiveness of ZOUSH in second degree burn wound healing in terms of histopathology and changes in the expression of some genes involved in wound healing process

This study is performed to evaluate the effect of local, inexpensive, standard and effective ZOUSH drug on the healing of second degree burn wounds through macroscopic and microscopic examination (histopathological) of the samples and to investigate the changes in the expression level of genes involved in this repair.

Clinical trials are conducted in parallel, In this study, the case and control are both the same patient.The study is a one-way blind and phase 3 and is randomized in the sampling area. Excel function rand function is used for randomization.

This study is performed in Shahid Motahari Hospital. Sampling of each patient in the burn area is done before and after treatment. In this study, the patient, pathologist and geneticist were blinded. The patient is unaware of the type of treatment in the treated areas and the pathologist and geneticist have been blinded to the type of sample received.

Inclusion criteria:the presence of second degree burns in the patient referred to Shahid Motahari Hospital;their willingness to participate in the study;complete the informed consent form. Exclusion criteria:patients' unwillingness to participate in the study;the presence of first or third degree burns, a history of trauma or previous burns in the burned area;facial burns, which are aesthetically important.

In this study, 20 patients are sampled in three areas. Due to pre-treatment sampling, after treatment in the ZOUSH-treated area and in the amniotic-treated area, each patient can be considered as a control for himself.

One of the goals of the Trauma Research Center is to find new and local treatments for burn trauma. In this project, a cheap and effective local medicine for the treatment of burn wounds will be genetically and histopathologically examined.

A small portion of the patient's wound is randomly selected and treated with ZOUSH. The rest of the wound is treated with standard amniotic dressing. By accident, here means that the burn is not intended for a specific area of the body. In addition, a special part of the burned area is not considered. If there is a random burn in each organ, a part of it will be selected for treatment with ZOUSH.

The patient, pathologist, and genetic researcher are completely unaware of which area is receiving standard amniotic fluid treatment and which area is being treated with ZOUSH, and data analysis is performed by blinding.

At the end of the study, the results of the research will be publish as an article.

data will be available endless.

all people.

in order to extend information in the fields of burn patients treatment the results will be available without limitations.

paper publisher site.

one month later the request will be answered by the email.