Protocol summary

Study aim
In this study, investigators aimed to assess safety and efficasy of intrathecal injection of autologous mesenchymal stem cells in patients with Amyotrophic Lateral Sclerosis (ALS)
Design
Clinical trial without control group
Settings and conduct
Patients referred to neuromuscular clinic of Golestan hospital of Ahvaz are visited by a neurologist and patients with definite or probable ALS based on revised ELEscorial criteria will enter the study. All patients sign the informed consent prior to inclusion in the study. In Shafa hospital of Ahvaz bone marrow aspiration is performed and autologous mesenchymal stem cells are isolated. Patients are received bone marrow mesenchymal stem cells via lumbar puncture into the cerebrospinal fluid. Patients are monitored before and after treatment, potential adverse reactions are assessed, and the clinical outcome is evaluated by Revised ALS Functional Rating Scale (ALSFRS-R) and Forced Vital Capacity (FVC)
Participants/Inclusion and exclusion criteria
Inclusion criteria: Patients diagnosed as definite or probable ALS disease according to ELEscorial criteria, Time passed since onset of symtoms till inclusion be 3 years or less, ALSFRS-R score more than 26, FVC more than 40%, An age range of 25 to 70 years; Exclusion criteria: any major systemic disease, any coexisting neurodegenerative disease, pregnancy or lactation
Intervention groups
Eligible patients are received bone marrow mesenchymal stem cells via lumbar puncture into the cerebrospinal fluid.
Main outcome variables
ALSFRS-R evaluation: using standard questionnaires completed by neurologist. FVC measurement: using spirometry test

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT20200828048551N1
Registration date: 2020-09-09, 1399/06/19
Registration timing: registered_while_recruiting

Last update: 2020-09-09, 1399/06/19
Update count: 0
Registration date
2020-09-09, 1399/06/19
Registrant information
Name
Fatemeh Houshmand
Name of organization / entity
Country
Iran (Islamic Republic of)
Phone
+98 61 3374 3001
Email address
hooshmandf66@yahoo.com
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2020-02-24, 1398/12/05
Expected recruitment end date
2020-09-26, 1399/07/05
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
Efficasy and safety of intrathecal injection of autologous mesenchymal stem cells in patients with amyotrophic lateral sclerosis (ALS)
Public title
Efficasy and safety of intrathecal injection of autologous mesenchymal stem cells in patients with amyotrophic lateral sclerosis (ALS)
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Patients diagnosed as definite or probable ALS disease according to EL Escorial criteria ALSFRS-R more than 26 FVC more than 40% age 25-70 years Time passed since onset of the symptoms till inclusion be 3 years or less
Exclusion criteria:
Any major systemic disease Any coexisting neurodegenerative disease No other major pathology in brain or spinal cord pregnancy or lactation
Age
From 25 years old to 70 years old
Gender
Both
Phase
2
Groups that have been masked
No information
Sample size
Target sample size: 10
Randomization (investigator's opinion)
N/A
Randomization description
Blinding (investigator's opinion)
Not blinded
Blinding description
Placebo
Not used
Assignment
Single
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Ahvaz University of Medical Sciences
Street address
Ahvaz university of medical sciences, Farvardin Ave, Ahvaz
City
Ahvaz
Province
Khouzestan
Postal code
6135715794
Approval date
2020-02-22, 1398/12/03
Ethics committee reference number
IR.AJUMS.REC.1398.904

Health conditions studied

1

Description of health condition studied
Amyotrophic lateral sclerosis
ICD-10 code
G12.21
ICD-10 code description
Amyotrophic lateral sclerosis

Primary outcomes

1

Description
Disability
Timepoint
3 months
Method of measurement
ALSFRS-R

2

Description
Forced vital capacity
Timepoint
3 months
Method of measurement
spirometry

3

Description
Side effects
Timepoint
3 months
Method of measurement
Questionnaire

Secondary outcomes

empty

Intervention groups

1

Description
Intrathecal injection of mesenchymal stem cells
Category
Treatment - Other

Recruitment centers

1

Recruitment center
Name of recruitment center
Golestan hospital
Full name of responsible person
Fatemeh Houshmand
Street address
EMG Departement, Golestan hospital, Farvardin Ave, Ahvaz
City
Ahvaz
Province
Khouzestan
Postal code
6135715794
Phone
+98 61 3374 3001
Email
hooshmandf66@yahoo.com

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Ahvaz University of Medical Sciences
Full name of responsible person
Dr mohammad badavi
Street address
Ahvaz University of Medical Science, Farvardin Ave, Ahvaz
City
Ahvaz
Province
Khouzestan
Postal code
6135715794
Phone
+98 61 3373 8383
Email
research@ajums.ac.ir
Web page address
http://www.ajums.ac.ir
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Ahvaz University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Ahvaz University of Medical Sciences
Full name of responsible person
Dr Gholamreza Shamsaei
Position
Associate professor
Latest degree
Subspecialist
Other areas of specialty/work
Neurology
Street address
Golestan Hospital, Farvardin Ave, Ahvaz
City
Ahvaz
Province
Khouzestan
Postal code
6135715794
Phone
+98 61 3374 3001
Email
Shamsaei-gh@ajums.ac.ir

Person responsible for scientific inquiries

Contact
Name of organization / entity
Ahvaz University of Medical Sciences
Full name of responsible person
Dr Ahmad Ahmadzade Deylami
Position
Associate professor
Latest degree
Subspecialist
Other areas of specialty/work
Hematology
Street address
Shafa Hospital, Farvardin Ave, Ahvaz
City
Ahvaz
Province
Khouzestan
Postal code
6135715794
Phone
+98 61 3374 3281
Email
Ahmad.ahmadzadeh@gmail.com

Person responsible for updating data

Contact
Name of organization / entity
Ahvaz University of Medical Sciences
Full name of responsible person
Fatemeh Houshmand
Position
Resident
Latest degree
Medical doctor
Other areas of specialty/work
Neurology
Street address
Golestan Hospital, Farvardin Ave, Ahvaz
City
Ahvaz
Province
Khouzestan
Postal code
6135715794
Phone
+98 61 3374 3001
Email
Hooshmandf66@yahoo.com

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Undecided - It is not yet known if there will be a plan to make this available
Study Protocol
Undecided - It is not yet known if there will be a plan to make this available
Statistical Analysis Plan
Undecided - It is not yet known if there will be a plan to make this available
Informed Consent Form
Undecided - It is not yet known if there will be a plan to make this available
Clinical Study Report
Undecided - It is not yet known if there will be a plan to make this available
Analytic Code
Undecided - It is not yet known if there will be a plan to make this available
Data Dictionary
Undecided - It is not yet known if there will be a plan to make this available
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