Protocol summary

Study aim
The aim of this study was to measure the serum levels of cytokines including interleukin 6, interleukin 10 and TNFα in general and spinal anesthesia and compare them with each other in cesarean section.
Design
Clinical trial, with a parallel group design, with blinded outcome assessment, randomized using random number table, performs on 30 patients between April 2020 and March 2021.
Settings and conduct
30 patients undergoing cesarean section who refer to Taleghani Hospital are randomly assigned into two similar groups of 15 people of spinal anesthesia and general anesthesia. In the spinal anesthesia group, 2.8 cc of 0.5% Marcaine is injected into the L3-L4 or L4-L5 space. In the general anesthesia group, endotracheal intubation is performed with thiopental 4-6 mg / kg and succinylcholine 1-1.5 mg / kg . It is then continued with nitrous oxide 50 % and 0.8% isoflurane. After delivery of baby, fentanyl 200µgr per 70 kg is injected. Both groups are given two diclofenac suppositories of 100 mg after anesthesia to control postoperative pain.
Participants/Inclusion and exclusion criteria
All patients scheduled for cesarean section, who have completed the consent form and are ASA Class I, II, are included in the study. Patients with coagulation disorders and phospholipid syndrome are excluded from the study.
Intervention groups
Two groups of general and spinal anesthesia .
Main outcome variables
Interlukin-6؛ Interlukin10؛ TNFα

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT20200930048887N1
Registration date: 2020-10-13, 1399/07/22
Registration timing: registered_while_recruiting

Last update: 2020-10-13, 1399/07/22
Update count: 0
Registration date
2020-10-13, 1399/07/22
Registrant information
Name
Maryam Vosoughian
Name of organization / entity
Country
Iran (Islamic Republic of)
Phone
+98 21 2243 2595
Email address
maryam.vosoghian@mail.sbmu.ac.ir
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2020-04-20, 1399/02/01
Expected recruitment end date
2021-03-05, 1399/12/15
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
Comparison of the effect of general anesthesia and spinal anesthesia on serum cytokines in cesarean section patients
Public title
The effect of type of anesthesia method on serum cytokines in cesarean section
Purpose
Prevention
Inclusion/Exclusion criteria
Inclusion criteria:
All patients refer to Taleghani Hospital for cesarean section Patients who fill out the consent form ASA Class I, II patients
Exclusion criteria:
Patients with any coagulation disorder Patients with antiphospholipid syndrome
Age
No age limit
Gender
Female
Phase
N/A
Groups that have been masked
  • Outcome assessor
  • Data analyser
Sample size
Target sample size: 30
Randomization (investigator's opinion)
Randomized
Randomization description
Participants are randomly divided into study groups 1 or 2 using a random number table. The names of the assigned group are written on the cards and placed in numbered and sealed envelopes. When each eligible woman enters the study, a numbered envelope is opened in sequence and the group is assigned.
Blinding (investigator's opinion)
Single blinded
Blinding description
A person who is not involved in the study opens the numbered envelope in sequence. Laboratory staff and data analysts are also unaware of the assignment of groups.
Placebo
Not used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Shahid Beheshti University of Medical Sciences
Street address
Taleghani Hospital, Velenjac Ave., Yaman St., Arabi st., Tehran, Iran.
City
Tehran
Province
Tehran
Postal code
1985717443
Approval date
2019-05-14, 1398/02/24
Ethics committee reference number
IR.SBMU.MSP.REC.1398.169

Health conditions studied

1

Description of health condition studied
Serum cytokines level
ICD-10 code
ICD-10 code description

Primary outcomes

1

Description
Serum cytokines level
Timepoint
Measurement of cytokines before cesarean section and half an hour after recovery
Method of measurement
ELIZA method

Secondary outcomes

empty

Intervention groups

1

Description
Intervention group:. In spinal anesthesia group, 2.8 cc of 0.5% Marcaine(Bupivacaine MYLAM SANT PRIEST-FRANCE) is injected into the L3-L4 or L4-L5 space.
Category
Treatment - Other

2

Description
Intervention group: . In the general anesthesia group, rapid sequence induction by thiopental 4-6 mg / kg and succinylcholine 1-1.5 mg / kg and endotracheal intubation is performed . Then oxygen and nitrous oxide 50% and 0.8% isoflurane is continued . After delivery of baby, fentanyl 200µgr per 70 kg is injected.
Category
Treatment - Other

Recruitment centers

1

Recruitment center
Name of recruitment center
Talegani Hospital
Full name of responsible person
Maryam Vosoughian
Street address
Talegani Hospital, Velenjac Ave., Yaman St., Arabi st., Tehran, Iran.
City
Tehran
Province
Tehran
Postal code
1985711151
Phone
+98 21 2243 2595
Fax
+98 21 2243 2570
Email
maryam.vosoghian@mail.sbmu.ac.ir

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Shahid Beheshti University of Medical Sciences
Full name of responsible person
Ali ِِDabbagh
Street address
Talegani Hospital, Velenjac Ave., Yaman St., Arabi st., Tehran, Iran.
City
Tehran
Province
Tehran
Postal code
1985711151
Phone
+98 21 2243 2595
Fax
+98 21 2243 2570
Email
alidabbagh@yahoo.com
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Shahid Beheshti University of Medical Sciences
Proportion provided by this source
30
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Shahid Beheshti University of Medical Sciences
Full name of responsible person
Maryam Vosoughian
Position
Associated Professor
Latest degree
Specialist
Other areas of specialty/work
Anesthesiology
Street address
Talegani Hospital, Velenjac Ave., Yaman St., Arabi st., Tehran, Iran
City
Tehran
Province
Tehran
Postal code
1985711151
Phone
+98 21 2243 2595
Email
maryam.vosoghian@mail.sbmu.ac.ir

Person responsible for scientific inquiries

Contact
Name of organization / entity
Shahid Beheshti University of Medical Sciences
Full name of responsible person
Maryam Vosoughian
Position
Associate Professor
Latest degree
Specialist
Other areas of specialty/work
Anesthesiology
Street address
Talegani Hospital, Velenjac Ave., Yaman St., Arabi st., Tehran, Iran
City
Tehran
Province
Tehran
Postal code
1985711151
Phone
+98 21 2243 2595
Email
maryam.vosoghian@mail.sbmu.ac.ir

Person responsible for updating data

Contact
Name of organization / entity
Shahid Beheshti University of Medical Sciences
Full name of responsible person
Maryam Vosoughian
Position
Associate Professor
Latest degree
Specialist
Other areas of specialty/work
Anesthesiology
Street address
Talegani Hospital, Velenjac Ave., Yaman St., Arabi st., Tehran, Iran
City
Tehran
Province
Tehran
Postal code
1985711151
Phone
+98 21 2243 2595
Email
maryam.vosoghian@mail.sbmu.ac.ir

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Yes - There is a plan to make this available
Study Protocol
Yes - There is a plan to make this available
Statistical Analysis Plan
Yes - There is a plan to make this available
Informed Consent Form
Undecided - It is not yet known if there will be a plan to make this available
Clinical Study Report
Yes - There is a plan to make this available
Analytic Code
Not applicable
Data Dictionary
Not applicable
Title and more details about the data/document
Only part of the data, such as information about the main outcome or the like, can be shared.
When the data will become available and for how long
The access period will start 8 months after the results are published.
To whom data/document is available
The data will be available only to researchers working in academic and scientific institutions.
Under which criteria data/document could be used
The use of the results for additional studies is allowed with the permission of the responsible author.
From where data/document is obtainable
Maryam Vosoughian Talegani Hospital, Velenjac Ave., Yaman St., Arabi st., Tehran, Iran maryam.vosoghian@mail.sbmu.ac.ir 0098 9121386084
What processes are involved for a request to access data/document
Documents will be sent to the applicant no later than one month after contacting the author by e-mail.
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