Protocol summary

Study aim
The comparison of letrozole with estradiol on the outcomes of frozen embryo transfer cycles in women with polycystic ovarian syndrome
Design
A randomized controlled clinical trial, with parallel groups, single blinded, phase 3, with simple randomization method on 510 patients.
Settings and conduct
This study will be carried out at Yas Hospital (Tehran, Iran) on women with polycystic ovarian syndrome. The patients, the sonographer and the physician who will be responsible for administration of drugs will know about the interventions; the physician who will be responsible for embryo transferring and the statistician are unaware of the interventions.
Participants/Inclusion and exclusion criteria
Inclusion criteria of study: The women with polycystic ovarian syndrome; 18 - 40 years old; candidate of frozen embryo transfer Exclusion criteria of study: The other causes of hyperandrogenism and ovarian dysfunction; Basal FSH level >10 IU/L; History of recurrent miscarriage; History of congenital or acquired uterine malformations; History of male factor infertility
Intervention groups
In the control group, endometrial preparation will be done by traditional method with estrogen and progesterone containing drugs. In the study group, endometrial preparation will be done by letrozole.
Main outcome variables
Live Birth Rate

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT20150105020558N3
Registration date: 2021-03-02, 1399/12/12
Registration timing: prospective

Last update: 2021-03-02, 1399/12/12
Update count: 0
Registration date
2021-03-02, 1399/12/12
Registrant information
Name
Mahbod Ebrahimi
Name of organization / entity
Tehran University of Medical Sciences
Country
Iran (Islamic Republic of)
Phone
+98 21 8882 7794
Email address
maebrahimi@tums.ac.ir
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2021-04-21, 1400/02/01
Expected recruitment end date
2022-10-22, 1401/07/30
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
Comparison of the Effectiveness of Letrozole with Estradiol on Frozen Embryo Transfer Cycle Outcomes in Women with Polycystic Ovarian Syndrome
Public title
Letrozole effect in IVF cycles
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
The patients with impression of polycystic ovarian syndrome according to Rotterdam consensus as fulfilling at least two of the three criteria: 1- oligo/anovulation; 2- clinical or biochemical signs of hyperandrogenism; and 3- polycystic ovarian morphology on ultrasound Female age: 18 - 40 years old The candidate of frozen embryo transfer
Exclusion criteria:
The other causes of hyperandrogenism and ovarian dysfunction Basal FSH level >10 IU/L History of recurrent miscarriage History of congenital or acquired uterine malformations History of male factor infertility
Age
From 18 years old to 40 years old
Gender
Female
Phase
3
Groups that have been masked
  • Care provider
  • Outcome assessor
  • Data analyser
Sample size
Target sample size: 510
Randomization (investigator's opinion)
Randomized
Randomization description
Random allocation, there are 255 small envelopes containing letter A and 255 small envelopes in the same shape containing letter B in a bag; and for each patient one envelope is selected randomly.
Blinding (investigator's opinion)
Single blinded
Blinding description
Whilst the patients, the sonographer and the physician who will be responsible for administration of drugs will know about the interventions; the physician who will be responsible for embryo transferring and the statistician are unaware of the interventions.
Placebo
Not used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Sina Hospital
Street address
Yas Complex Hospital, Forth Alley Corner, North Ostad Nejatollahi Avenue, Karimkhan Street, Tehran, Iran
City
Tehran
Province
Tehran
Postal code
1597856511
Approval date
2020-12-14, 1399/09/24
Ethics committee reference number
IR.TUMS.SINAHOSPITAL.REC.1399.084

Health conditions studied

1

Description of health condition studied
Polycystic ovarian syndrome
ICD-10 code
E28.2
ICD-10 code description
Polycystic ovarian syndrome

Primary outcomes

1

Description
Live Birth Rate
Timepoint
After pregnancy termination
Method of measurement
The number of deliveries that resulted in a live born neonate, expressed per 100 embryo transfers

Secondary outcomes

1

Description
Clinical Pregnancy Rate
Timepoint
4 to 6 weeks after frozen embryo transfer
Method of measurement
A visible gestational sac by transvaginal ultrasound

2

Description
Ongoing Pregnancy Rate
Timepoint
After 20th weeks of pregnancy
Method of measurement
The number of fetuses with heart activity beyond 20 weeks of gestation

3

Description
Cycle cancellation rate
Timepoint
from 15th until 21th days of cycle
Method of measurement
The number of cancelled cycles

4

Description
Endometrial thickness
Timepoint
On the day of HCG injection
Method of measurement
The measuring of endometrial thickness by transvaginal ultrasound

5

Description
Abortion rate
Timepoint
From 5th until 24th gestational weeks
Method of measurement
The detection of lack or loss of fetal heart activity by ultrasound

6

Description
Premature delivery rate
Timepoint
From 25th until 36th gestational weeks
Method of measurement
The delivery of fetus(es)

Intervention groups

1

Description
Intervention group: In this group, two letrozole tablets (2.5 mg, َAburaihan pharmaceutical co) is prescribed orally for 5 days initiating on day 3 of spontaneous menses or progesterone-induced withdrawal bleeding. Ultrasound monitoring are performed from cycle day 10 onwards. if the leading follicle reaches a mean diameter of ≥ 14mm on cycle day 10, ultrasound is repeated every 2 days. In case of a mean dominant follicle diameter < 14mm on day 10, a daily dosage of 75 IU hMG is supplemented to stimulate follicle growth. When the dominant follicle reaches a mean diameter of ≥17mm and endometrial thickness reaches ≥ 7mm, a bolus of urinary hCG 5000 IU will be injected. According to embryo staging (cleavage stage or blastocyst), embryo transferring will be scheduled 4 or 6 days later.
Category
Treatment - Drugs

2

Description
Control group: In this group, three Estradiol Valerate tablets (2 mg, َAburaihan pharmaceutical co) are prescribed orally from day 3 of spontaneous menses or progesterone-induced withdrawal bleeding. Ultrasound monitoring are performed 12 to 14 days later. ultrasound examination is carried out to measure endometrial thickness as well as to ensure there is no dominant follicle. When the endometrial thickness attained ≥ 7mm, progestrone vaginal suppositories (Fertigest, 400mg, Aburaihan pharmaceutical co) twice daily are initiated. According to embryo staging (cleavage stage or blastocyst), embryo transferring will be scheduled 4 or 6 days later.
Category
Treatment - Drugs

Recruitment centers

1

Recruitment center
Name of recruitment center
Yas Hospital
Full name of responsible person
Mahbod Ebrahimi
Street address
Yas Hospital, Ostad Nejatolahi Ave., Karimkhan Blvd.
City
Tehran
Province
Tehran
Postal code
1598718311
Phone
+98 21 4216 0000
Email
maeb214@yahoo.com

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Tehran University of Medical Sciences
Full name of responsible person
Mahbod Ebrahimi
Street address
Keshavarz Blvd , Khods St , Tehran University of Medical Sciences
City
Tehran
Province
Tehran
Postal code
1597856511
Phone
+98 21 8608 9076
Email
maeb214@yahoo.com
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Tehran University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Tehran University of Medical Sciences
Full name of responsible person
Mahbod Ebrahimi
Position
Associate professor
Latest degree
Subspecialist
Other areas of specialty/work
Gynecology and Obstetrics
Street address
Yas Hospital, Ostad Nejatolahi Ave., Karimkhan Blvd.
City
Tehran
Province
Tehran
Postal code
1598718311
Phone
+98 21 4216 0000
Email
maeb214@yahoo.com

Person responsible for scientific inquiries

Contact
Name of organization / entity
Tehran University of Medical Sciences
Full name of responsible person
Mahbod Ebrahimi
Position
Associate professor
Latest degree
Subspecialist
Other areas of specialty/work
Gynecology and Obstetrics
Street address
Yas Hospital, Ostad Nejatolahi Ave., Karimkhan Blvd.
City
Tehran
Province
Tehran
Postal code
1598718311
Phone
+98 21 4216 0000
Email
maeb214@yahoo.com

Person responsible for updating data

Contact
Name of organization / entity
Tehran University of Medical Sciences
Full name of responsible person
Mahbod Ebrahimi
Position
Associate professor
Latest degree
Subspecialist
Other areas of specialty/work
Gynecology and Obstetrics
Street address
Moheb Yas Hospital , Oustad Nejatollahi St.
City
Tehran
Province
Tehran
Postal code
1598718311
Phone
+98 21 8882 7794
Fax
Email
maebrahimi@tums.ac.ir

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Yes - There is a plan to make this available
Study Protocol
Yes - There is a plan to make this available
Statistical Analysis Plan
Yes - There is a plan to make this available
Informed Consent Form
Yes - There is a plan to make this available
Clinical Study Report
Yes - There is a plan to make this available
Analytic Code
Yes - There is a plan to make this available
Data Dictionary
Yes - There is a plan to make this available
Title and more details about the data/document
Project report to Deputy of Research of TUMS, every researcher who needs data for research
When the data will become available and for how long
5 years
To whom data/document is available
Deputy of Research of TUMS, every researcher who needs data for research.
Under which criteria data/document could be used
After final report and publishing the results
From where data/document is obtainable
Miss Moamaei-Deputy of Research of TUMS,fifth floor,Central building of TUMS,Ghods ave., Tel:00982164431
What processes are involved for a request to access data/document
Miss Moamaei-Deputy of Research of TUMS or send the request to maeb214@yahoo.com, responsible author, after one month they will be responded
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