Effects of acute and chronic aerobic exercise training on vascular and systemic inflammatory biomarkers in patients with coronary artery disease after revascularization interventions

The aim of this study is to evaluate effects of acute and chronic exercise on vascular and systemic inflammatory biomarkers in patients with coronary artery disease after revascularization interventions (percutaneous coronary intervention (PCI) and coronary artery bypass grafting (CABG).It was hypothesized that C-reactive protein (CRP) is a biomarker of systemic and vascular adhesion molecules-1(VCAM-1) and pentraxin -3(PTX3) are markers of vascular inflammation. This is a clinical trial study. 70 male patients refers to Shahid Beheshti Hospital of Kashan University of Medical Science with history of coronary artery disease from 11/1/2017 will enroll in our study and will randomly assigned to in intervention group(IG) and control group(CG). IG will participate in one session submaximal aerobic exercise training and then 8 weeks of cardiac rehabilitation program and CG will have a normal daily activities. Before the beginning study, patients will perform a symptom limited exercise test based on Bruce protocol and their maximal heart rate and vo2 peak will be measured. Exercise intensity will be set between 65 to 75 percent of maximal heart rate. One day before the study start, patients will familiar with cardiac rehabilitation instrument and perform exercise test. After 24 hours patients will prepare to participate in acute submaximal aerobic exercise session include 15minutes warm up, 35 minute submaximal aerobic exercise on treadmill and ergometer and 10 minutes cool down. Patients in IG performed 8 weeks cardiac rehabilitation program(CRP) at 70 % of maximum heart rate,3-4 times a week each session 60 min consist of 15 min warm-up, 30 min submaximal aerobic exercise on tread mill and ergo meter and at the end 10 min cool down, Blood blood samples will obtain from the antecubital vein at baseline, 30 min after the of aerobic submaximal session and after 8 weeks cardiac rehabilitation program to measure plasma levels of pentraxin 3(PTX3), vascular cell adhesion molecule 1 ( VCAM-1), C-reactive protein (CRP) and lipid profile.The blood collect in chilled tube with EDTA and then centrifuge at 2000 *g for 15 min at 4 C then plasma will store at -20 c refrigerator. Inflammatory biomarkers will determine by means of enzyme-linked immunosurbent assay kits.

Mohaghegh Ardebili University, Kashan University of Medical Sciences