Protocol summary

Study aim
Determining the effectiveness of Lactobacillus reuteri in the treatment of functional abdominal pain in children
Design
180 children with functional abdominal pain, one group of 90 people receiving Lactobacillus reuteri probiotic and another 90 children as a control group, receive a placebo. This is a double-blind study. Random allocation software is used to randomize and each code is typed on the medicine package that the doctor will give the patient a complete questionnaire and description
Settings and conduct
Reuteflor packages are coded after randomization and are given to the relevant doctor in Mofid Children's hospital in Tehran and Besat in Hamedan or the private office and then to the patients. The doctor, patients, and their parents are blinded. The packages of both study groups are quite similar.
Participants/Inclusion and exclusion criteria
The boy or girl is 5 to 15 years old. Admission is based on the Rome 4 criteria. 1. Abdominal pain is persistent or almost constant. 2. Pain has nothing to do with physiological events in the body (eating, defecation, menstruation, etc.). 3. Some daily functions are lost. 4. Pain is not fake. 5. Symptoms to complement the criteria for other gastrointestinal functional disorders do not fully explain the pain. All of these criteria must be present for at least three months and the onset of symptoms at least six months before diagnosis. The child should not have an underlying disease that causes abdominal pain that is diagnosed by a pediatric gastroenterologist.
Intervention groups
A group of 90 patients receive a probiotic sachet every day for 28 days. A control group of 90 patients receive a placebo sachet with the same original packaging every day for 28 days.
Main outcome variables
Pain intensity; Pain frequency

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT20150706023084N14
Registration date: 2021-12-30, 1400/10/09
Registration timing: prospective

Last update: 2021-12-30, 1400/10/09
Update count: 0
Registration date
2021-12-30, 1400/10/09
Registrant information
Name
MARYAM SHIEHMORTEZA
Name of organization / entity
AZAD UNIVERSITY PHARMACEUTICAL SCIENCES
Country
Iran (Islamic Republic of)
Phone
+98 212640056
Email address
shiehmorteza@iaups.ac.ir
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2022-01-21, 1400/11/01
Expected recruitment end date
2022-08-23, 1401/06/01
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
Evaluation of the effectiveness of Lactobacillus reuteri probiotics in the treatment of chronic functional abdominal pain in children aged 5 to 15 years
Public title
Evaluation of the probiotic effect of Lactobacillus reuteri in the treatment of chronic functional pain in children
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Abdominal pain is persistent or almost constant Pain has nothing to do with physiological events in the body (eating, defecation, menstruation, etc.) Some daily functions are lost The pain is not fake Symptoms to complete the criteria for other gastrointestinal functional disorders do not justify complete pain All of these criteria must be present for at least three months and the onset of symptoms at least six months before diagnosis
Exclusion criteria:
The doctor determines that the child has chronic abdominal pain due to the consumption of a certain type of food (special diet) or a specific organic problem. Existence of an underlying disease that, according to the pediatric gastroenterologist, has caused abdominal pain.
Age
From 5 years old to 15 years old
Gender
Both
Phase
3
Groups that have been masked
  • Participant
  • Care provider
  • Investigator
  • Outcome assessor
Sample size
Target sample size: 180
Randomization (investigator's opinion)
Randomized
Randomization description
The identical packages of probiotic sachets or placebo (identified by codes 1 or 2) are randomly distributed among the people. The doctor randomly gives the patient a pack using the lottery method.
Blinding (investigator's opinion)
Double blinded
Blinding description
This study is performed in a double-blind manner so that neither the physician nor the patients are aware of the contents of the sachets received by the patients. The packages are identified by codes that do not show the content. In the end, the analyzer, who is the only person who knows each code's meaning, reviews and evaluates the findings.
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Department of Pharmaceutical Sciences, Tehran Islamic Azad University of Medical
Street address
Dr Shariati Ave., Gholhak, Yakhchal Ave., Islamic Azad University of Pharmaceutical Sciences Branch
City
Tehran
Province
Tehran
Postal code
193956466
Approval date
2021-11-10, 1400/08/19
Ethics committee reference number
IR.IAU.PS.REC.1400.301

Health conditions studied

1

Description of health condition studied
chronic functional abdominal pain in children
ICD-10 code
R10.4
ICD-10 code description
Other and unspecified abdominal pain

Primary outcomes

1

Description
Intensity of pain
Timepoint
Every day for 28 days
Method of measurement
Based on oucher pain scale

2

Description
Frequent pain
Timepoint
Every day for 28 days
Method of measurement
Record in the table

3

Description
Duration of treatment
Timepoint
28 days
Method of measurement
Check the questionnaire table

Secondary outcomes

empty

Intervention groups

1

Description
Intervention group: Lactobacillus reuteri probiotic. 28 sachets One gram of probiotic is dissolved in sachets of water, yogurt or food (cold) every day and consumed for 28 days. Each sachet of 1 g of Reuteflor contains 300 mg of Lactobacillus reuteri lyophilized active cell (equivalent to 8 ^ 10 × 8 CFU). Other ingredients: maltodextrin, cornstarch, magnesium stearate, silicon dioxide, inulin and natural banana flavoring. This product is free of gluten, talc, sweetener and milk ingredients.The company manufactures probiotics and placebos is farabiotic, both of which are delivered in exactly the same shape. The doctor will give the questionnaire along with the medicine to the patient with a full explanation. Store at a cool, dry temperature (below 25 ° C). No need to store in the refrigerator.
Category
Treatment - Drugs

2

Description
Control group: Lactobacillus reuteri placebo. 28 sachets One gram of placebo dissolves one sachet in water, yogurt or food (cold) every day and is consumed for up to 28 days. Each 1 gram placebo sachet contains maltodextrin, cornstarch, magnesium stearate, silicon dioxide, inulin and natural banana flavoring. This product is free of gluten, talc, sweetener and milk ingredients.The company manufactures probiotics and placebos is farabiotic, both of which are delivered in exactly the same shape. The doctor will give the questionnaire along with the medicine to the patient with a full explanation. Store at a cool, dry temperature (below 25 ° C). No need to store in the refrigerator.
Category
Placebo

Recruitment centers

1

Recruitment center
Name of recruitment center
Mofid children's Hospital
Full name of responsible person
Naghi Dara
Street address
Mofid Children's Hospital,Shariati Avenue, Tehran, Iran.
City
Tehran
Province
Tehran
Postal code
1551415468
Phone
+98 21 2670 3625
Email
info-mch@sbmu.ac.ir

2

Recruitment center
Name of recruitment center
Besat in Hamedan
Full name of responsible person
Gholamreza Kalvandi
Street address
Hamedan Shahid Motahari Boulevard, Hakma Street
City
Tehran
Province
Tehran
Postal code
6516795547
Phone
+98 81 3251 9147
Fax
+98 81 3251 9147
Email
besat@umsha.ac.ir

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Faradaro Fanavar Mehr Company
Full name of responsible person
Mohamad Mohamadi
Street address
North Kargar Street, next to the University of Tehran dormitory, Center for the Development of Pharmaceutical Technology Units, No. 1462
City
Tehran
Province
Tehran
Postal code
1439955991
Phone
+98 21 8835 9880
Email
info@faradaru.com
Web page address
https://farabiotic.com/
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Faradaro Fanavar Mehr Company
Proportion provided by this source
70
Public or private sector
Private
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Industry

Person responsible for general inquiries

Contact
Name of organization / entity
Islamic Azad University
Full name of responsible person
Maryam Shiehmorteza
Position
Assistant Professor
Latest degree
Ph.D.
Other areas of specialty/work
Medical Pharmacy
Street address
Yakhchal ,Shariati Street
City
Tehran
Province
Tehran
Postal code
646619395
Phone
+98 212640056
Fax
+98 21 2264 0056
Email
shiehmorteza@iaups.ac.ir

Person responsible for scientific inquiries

Contact
Name of organization / entity
Islamic Azad University
Full name of responsible person
Maryam Shiehmorteza
Position
assistant professor
Latest degree
Ph.D.
Other areas of specialty/work
Medical Pharmacy
Street address
No.99, Yakhchal street, Dr. Shariati Avenue
City
tehran
Province
Tehran
Postal code
1916893813
Phone
+98 21 2264 0056
Fax
Email
shiehmorteza@iaups.ac.ir
Web page address

Person responsible for updating data

Contact
Name of organization / entity
Islamic Azad University
Full name of responsible person
Ahmadreza Malekshahi
Position
Pharmacy student
Latest degree
A Level or less
Other areas of specialty/work
Medical Pharmacy
Street address
No.99, Yakhchal street, Dr. Shariati Avenue
City
Tehran
Province
Tehran
Postal code
1916893813
Phone
+98 21 2264 0056
Fax
Email
ahmadreza77mk@gmail.com
Web page address

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Yes - There is a plan to make this available
Study Protocol
Yes - There is a plan to make this available
Statistical Analysis Plan
Yes - There is a plan to make this available
Informed Consent Form
Yes - There is a plan to make this available
Clinical Study Report
Yes - There is a plan to make this available
Analytic Code
Yes - There is a plan to make this available
Data Dictionary
Yes - There is a plan to make this available
Title and more details about the data/document
Data are collected in the form of a questionnaire
When the data will become available and for how long
After the intervention
To whom data/document is available
Qualified persons
Under which criteria data/document could be used
Use for scientific advancement in the field under study
From where data/document is obtainable
Clinical Office of Islamic Azad University, Medical Sciences of Tehran, Faculty of Pharmacy
What processes are involved for a request to access data/document
Clinical Office of Islamic Azad University, Medical Sciences of Tehran, Faculty of Pharmacy
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