Protocol summary

Study aim
Determining and comparing the effect of amnion dressing on healing superficial second-degree burns wounds
Design
Two arms parallel-group randomized trial with a control group and blinded participants and phase 2 on 58 patients. The blocking method was used for randomization.
Settings and conduct
This study is performed in the burn department of Imam Reza Hospital. 58 patients with deep second-degree burns (less than 20% of burns) who require skin grafts are randomly assigned into two groups based on clinical symptoms confirmed by an infectious disease specialist and a plastic and burn surgeon. Group A patients receive amnion dressing and then dry gauze and bandaging, and group B patients receive the usual treatment (a layer of fatty gauze, dry gauze, and then bandaging). Every 48 hours, the secondary dressing, which includes dry gauze and a bandage, is replaced, and the donor site is evaluated in terms of infection and the progress of wound healing. An infectious disease specialist and a plastic and burn surgeon perform monitor all patients.
Participants/Inclusion and exclusion criteria
Inclusion criteria: Patients between the ages of 15 and 55 are eligible to participate. Have a severe burn of grade 2 or higher that requires a skin graft Patients with burns that are less than or equal to 20% Patient with deep second degree burns in upper and lower limbs and anterior trunk except for lateral thigh Criteria for not including people in the study: Patients under 15 years and over 55 years Patients with more than 20% burns Burns in the lateral thigh area
Intervention groups
The intervention group consisted of 29 patients who underwent amnion dressing in their lateral thighs.
Main outcome variables
Duration of complete wound healing in the two groups

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT20210918052511N1
Registration date: 2021-09-24, 1400/07/02
Registration timing: prospective

Last update: 2021-09-24, 1400/07/02
Update count: 0
Registration date
2021-09-24, 1400/07/02
Registrant information
Name
Omid Yazarlu
Name of organization / entity
Country
Iran (Islamic Republic of)
Phone
+98 51 3841 2073
Email address
yazarlouom@mums.ac.ir
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2021-10-22, 1400/07/30
Expected recruitment end date
2022-02-19, 1400/11/30
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
Assessment of the efficacy of amnion dressing in wound healing of skin graft donor sites (superficial second-degree wound) in the lateral thigh and its comparison with conventional wound dressing
Public title
Assessment of the efficacy of amnion dressing in wound healing of skin graft donor sites
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Patients between the ages of 15 and 55 are eligible to participate. Have a severe burn of grade 2 or higher that requires a skin graft Patients with burns that are less than or equal to 20% Patient with deep second degree burns in upper and lower limbs and anterior trunk except lateral thigh
Exclusion criteria:
Patients who are receiving chemotherapy Patients taking corticosteroids or cytotoxic medicines Pregnancy Cancer, cytotoxic drugs, immunosuppressants, and the presence of chronic diseases other than diabetes that affect wound healing, such as severe vascular disease, lupus, rheumatoid arthritis, and renal failure Smoking The presence of an underlying disease leads to a defective immune system Diabetic patients Taking drugs that lead to a defective immune system. Burns in the lateral thigh area.
Age
From 15 years old to 55 years old
Gender
Both
Phase
N/A
Groups that have been masked
  • Participant
Sample size
Target sample size: 58
Randomization (investigator's opinion)
Randomized
Randomization description
Each of the 58 patients would be assigned a number between 1 and 58 which is randomized by using random.org/integers in two columns. ​The numbers of the first column are assigned to group A and the second column is assigned to group B. With a lottery method, each column (A or B) is assigned to intervention or control groups.
Blinding (investigator's opinion)
Single blinded
Blinding description
Prior to participating in the trial, all patients or their legal representatives sign an informed consent form and are informed that they will be in one of two treatment groups. After that, participants enter the operating room and receive the dressing based on how the allocation occurred, and they are unaware of the type of dressing received.
Placebo
Not used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
research ethics committee of school of medicine-Mashhad university of medical sciences
Street address
Azadi squer- campus of Medical University of Mashhad
City
Mashhad
Province
Razavi Khorasan
Postal code
91379-13316
Approval date
2021-08-15, 1400/05/24
Ethics committee reference number
IR.MUMS.MEDICAL.REC.1400.331

Health conditions studied

1

Description of health condition studied
Burn patients
ICD-10 code
T20
ICD-10 code description
Burn and corrosion of head, face, and neck

Primary outcomes

1

Description
Duration of complete wound healing
Timepoint
Every 48 hours
Method of measurement
Based on the clinical observations of an infectious disease specialist and plastic and burn surgeon

Secondary outcomes

1

Description
Wound condition, including the presence or absence (yes/no) of granulation tissue, bleeding, pain, infection, and other wound complications or healing factors are evaluated.
Timepoint
Every 48 hours
Method of measurement
Based on the scores of the variables and presence or absence (yes / no)

Intervention groups

1

Description
Intervention group: The amnion dressing is used for two weeks and this dressing is provided by Sinacell Co.Amnion membrane as a biological dressing in the treatment of burn wounds accelerates wound healing, reduces the need to change dressings, shorten the duration of hospitalization, reduce scar formation, temperature control, proper air permeability to the wound and reduce pain and earlier removal of the dressing . It is used once for dressing the donor site in the operating room and the secondary dressing (dry gauze and bandage) is changed every 48 hours.
Category
Treatment - Devices

2

Description
Control group: includes the conventional treatment (a layer of fatty gauze, dry gauze and then bandaging) for two weeks.
Category
Treatment - Devices

Recruitment centers

1

Recruitment center
Name of recruitment center
Burn department-Imam Reza hospital
Full name of responsible person
Dr. Omid Yazarlou
Street address
Daneshgah street-Burn department-Imam Reza hospital
City
Mashhad
Province
Razavi Khorasan
Postal code
91379-13316
Phone
+98 51 3802 2051
Fax
Email
Yazarlouom@mums.ac.ir

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Mashhad University of Medical Sciences
Full name of responsible person
Dr. Khalil Abnous
Street address
Daneshgah Street- Mashhad University of Medical Sciences
City
Mashhad
Province
Razavi Khorasan
Postal code
13944-91388
Phone
+98 51 3879 5031
Email
AbnousKh@mums.ac.ir
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
No
Title of funding source
Sina Cell Co.
Proportion provided by this source
100
Public or private sector
Private
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Industry

Person responsible for general inquiries

Contact
Name of organization / entity
Mashhad University of Medical Sciences
Full name of responsible person
Dr Omid Yazarlou
Position
Assistant Professor
Latest degree
Subspecialist
Other areas of specialty/work
Plastic & Reconstructive Surgery
Street address
Burn Department of Imam Reza Hospital
City
Mashhad
Province
Razavi Khorasan
Postal code
91379-13316
Phone
+98 51 3802 2051
Email
Yazarlouom@mums.ac.ir

Person responsible for scientific inquiries

Contact
Name of organization / entity
Mashhad University of Medical Sciences
Full name of responsible person
Dr. Omid Yazarlou
Position
Assistant Professor
Latest degree
Subspecialist
Other areas of specialty/work
Plastic & Reconstructive Surgery
Street address
Burn Department of Imam Reza Hospital
City
Mashhad
Province
Razavi Khorasan
Postal code
91379-13316
Phone
+98 51 3802 2051
Email
Yazarlouom@mums.ac.ir

Person responsible for updating data

Contact
Name of organization / entity
Mashhad University of Medical Sciences
Full name of responsible person
Dr Maede Hasanpour
Position
Post-doctoral fellowship
Latest degree
Ph.D.
Other areas of specialty/work
Medical Pharmacy
Street address
Azadi Square - Mashhad School of Pharmacy
City
Mashhad
Province
Razavi Khorasan
Postal code
91775-1365
Phone
+98 51 3180 1841
Email
maede.hasanpour@yahoo.com

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Yes - There is a plan to make this available
Study Protocol
Yes - There is a plan to make this available
Statistical Analysis Plan
Yes - There is a plan to make this available
Informed Consent Form
Yes - There is a plan to make this available
Clinical Study Report
Not applicable
Analytic Code
Not applicable
Data Dictionary
Not applicable
Title and more details about the data/document
Part of the data is related to the main outcome and the secondary outcome can be shared.
When the data will become available and for how long
Access period starts 3 months after the results are published
To whom data/document is available
The data is available to researchers working in academic and scientific institutions as well as people working in industry.
Under which criteria data/document could be used
Use as a reference
From where data/document is obtainable
Dr. Maede Hasanpour - Faculty of Pharmacy - Room 233 - Phon number 09113145617-maede.hasanpour@yahoo.com
What processes are involved for a request to access data/document
After making a call via email or phone, the desired data will be sent using email or post.
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