The effect of 8 weeks of high-intensity interval training (HIIT) with and without vitamin D supplementation on some muscle atrophy indexes in older men
Determining the effect of eight weeks of high-intensity interval training (HIIT), vitamin D supplementation and their combination on serum levels of GDF8, IGF-1, miRNA-1, miRNA-133a and miRNA-206 in inactive elderly men
Design
A controlled clinical trial with randomized parallel groups was performed on 40 inactive elderly men
Settings and conduct
For this purpose, in a quasi-experimental research project, 40 elderly people (60 to 75 years old) were considered as a research sample. Subjects will be randomly divided into four groups of 10: control, HIIT, HIIT + vitamin D exercise, and vitamin D group. The training protocol is performed three days a week for eight weeks. Vitamin D supplementation in the vitamin D group and the HIIT + vitamin D exercise group is estimated at 2000 International Units (IU) per day. Blood samples and functional tests are taken at rest 48 hours before the start of training and 48 hours after the last training session.
Participants/Inclusion and exclusion criteria
• Age range: 75-60 years
• inactive (less than 90 minutes of regular exercise per week)
• Do not use drugs and anabolic supplements, blood pressure, anti-inflammatory and antioxidants
Intervention groups
Subjects are divided into four groups.
1 - Control group that follow their daily schedule and diet.
2- Vitamin D group: which consumes 2000 international units of vitamin D per day and perform daily activities
3-high-intensity interval training group that performs these exercises three days a week for 8 weeks
4-high-intensity interval training group + vitamin D intake, this group 3 days a week of high-intensity interval training in addition to consuming 2000 international units of vitamins
The effect of 8 weeks of high-intensity interval training (HIIT) with and without vitamin D supplementation on some muscle atrophy indexes in older men
Public title
The effect of high-intensity interval training (HIIT) and vitamin D supplementation on older men
Purpose
Other
Inclusion/Exclusion criteria
Inclusion criteria:
Age range 60 to 75 years
inactive (less than 90 minutes of regular exercise per week)
Do not use drugs and anabolic supplements, blood pressure, anti-inflammatory and antioxidants
Exclusion criteria:
The elderly are injured
Metabolic and cardiovascular diseases
Age
From 60 years old to 75 years old
Gender
Male
Phase
N/A
Groups that have been masked
No information
Sample size
Target sample size:
40
Randomization (investigator's opinion)
Randomized
Randomization description
At first, the subjects will be selected using a simple random method. Then, using a table of random numbers, they are divided into four groups: control, exercise, vitamin D and exercise + vitamin D. Therefore, each candidate will be assigned a two-digit number from 01.02.000 to 40. Starting from a point on the table of random numbers in the direction of the desired row or column, the same number of digits is determined for the selection of individuals (five-digit numbers whose last two digits are similar to the existing codes) that are randomly assigned to one of the groups It will be given. This will continue until the number of people in each group is completed. Therefore, the researcher will not have the option to change the status of the assignment or predict it. Random hiding will be done by a third party who does not participate in other stages of the intervention.
Blinding (investigator's opinion)
Not blinded
Blinding description
Placebo
Not used
Assignment
Parallel
Other design features
Secondary Ids
empty
Ethics committees
1
Ethics committee
Name of ethics committee
Ethics committee of tabriz University
Street address
29 Bahman Blvd., University of Tabriz
City
tabriz
Province
East Azarbaijan
Postal code
5166616471
Approval date
2022-01-02, 1400/10/12
Ethics committee reference number
IR.TABRIZU.REC.1400.059
Health conditions studied
1
Description of health condition studied
Sarcopenia
ICD-10 code
M62.5
ICD-10 code description
Muscle wasting and atrophy, not elsewhere classified
Primary outcomes
1
Description
Serum levels of vitamin D
Timepoint
48 hours before and after 8 weeks of exercise training
Method of measurement
Using the ELISA method
2
Description
Serum levels of Insulin-like hormone 1
Timepoint
48 hours before and after 8 weeks of exercise training
Method of measurement
Using the ELISA method
3
Description
Serum levels of myostatin hormone
Timepoint
48 hours before and after 8 weeks of exercise training
Method of measurement
Using the ELISA method
4
Description
miRNA-1
Timepoint
48 hours before and after 8 weeks of exercise training
Method of measurement
Real Time PCR technique
5
Description
miRNA-133a
Timepoint
48 hours before and after 8 weeks of exercise training
Method of measurement
Real Time PCR technique
6
Description
miRNA-206
Timepoint
48 hours before and after 8 weeks of exercise training
Method of measurement
Real Time PCR technique
Secondary outcomes
empty
Intervention groups
1
Description
Control group: without receiving supplements and exercise program
Category
Other
2
Description
Intervention group: The daily intake of 2000 international units of vitamin D per day will be done for eight weeks.
Category
Other
3
Description
Intervention group: Group three (high-intensity interval training ): Subjects in the high-intensity interval training group will participate 3 days a week for 8 weeks of high-intensity interval training with an intensity of more than 75% of the reserve heart rate. Blood samples are taken at rest 48 hours before the start of training and 48 hours after the last training session.
Category
Other
4
Description
Intervention group: Group four (High-intensity interval training +Vitamin D group): Subjects in the high-intensity interval training group will take vitamin D, 3 days a week, for 8 weeks of high-intensity interval training with an intensity of more than 75% of the reserve heart rate. During this period, 2,000 international units of vitamin D will be consumed every day. Blood samples are taken at rest 48 hours before the start of training and 48 hours after the last training session.
Category
Other
Recruitment centers
1
Recruitment center
Name of recruitment center
The University of Tabriz
Full name of responsible person
Javad Vakili
Street address
29 Bahman Blvd., University of Tabriz
City
Tabriz
Province
East Azarbaijan
Postal code
5166616471
Phone
+98 41 3334 0081
Fax
+98 41 3334 4013
Email
vakili@tabrizu.ac.ir
Sponsors / Funding sources
1
Sponsor
Name of organization / entity
The University of tabriz
Full name of responsible person
Jalal Shiri
Street address
29 Bahman Blvd., University of Tabriz
City
Tabriz
Province
East Azarbaijan
Postal code
5166616471
Phone
+98 41 3334 0081
Fax
+98 41 3334 4013
Email
Jalalshiri@tabrizu.ac.ir
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
The University of tabriz
Proportion provided by this source
20
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic
Person responsible for general inquiries
Contact
Name of organization / entity
The University of tabriz
Full name of responsible person
Kaveh Baturak
Position
P.hd student
Latest degree
Master
Other areas of specialty/work
Sport Physiology
Street address
29 Bahman Blvd., University of Tabriz
City
Tabriz
Province
East Azarbaijan
Postal code
5166616471
Phone
+98 41 3334 0081
Fax
+98 41 3334 4013
Email
Kawa.Baturak@gmail.com
Person responsible for scientific inquiries
Contact
Name of organization / entity
The University of tabriz
Full name of responsible person
Javad Vakili
Position
Associate professor
Latest degree
Ph.D.
Other areas of specialty/work
Physiology
Street address
29 Bahman Blvd., University of Tabriz, Faculty of Physical Education and Sport Sciences
City
Tabriz
Province
East Azarbaijan
Postal code
5166616471
Phone
+98 41 3339 3257
Fax
+98 41 3339 3258
Email
Vakili@tabrizu.ac.ir
Person responsible for updating data
Contact
Name of organization / entity
The University of Tabriz
Full name of responsible person
Kaveh Baturak
Position
Ph.D student
Latest degree
Master
Other areas of specialty/work
Sport physiology
Street address
29 Bahman Blvd., University of Tabriz, Faculty of Physical Education and Sport Sciences
City
Tabriz
Province
East Azarbaijan
Postal code
5166616471
Phone
+98 41 3339 3257
Fax
+98 41 3339 3258
Email
Kawa.Baturak@gmail.com
Sharing plan
Deidentified Individual Participant Data Set (IPD)
No - There is not a plan to make this available
Justification/reason for indecision/not sharing IPD