Comparison of the effect of daily use of mouthwash and toothpaste containing post-biotic on the number of Streptococcus Mutans in the saliva of patients undergoing fixed orthodontic treatment.

Comparison of the effect of daily use of mouthwash and toothpaste containing post-biotic on the number of Streptococcus Mutans in the saliva of patients undergoing fixed orthodontic treatment

Institutional-based, parallel groups, triple-blind, randomized clinical trial. Assignment of patients to study groups will be done using the random block method and using 6 blocks.

The research site will be the School of Dentistry of the Ahvaz Jundshapur University of Medical Sciences. Sampling will be performed by the non-probability method. Outcome is measured before the intervention. Participants will be randomly divided into interventions and control groups and after the intervention, the outcome will be measured again. In this study, participants, the person evaluating the results and analyzer of the data will be blinded.

Inclusion criteria: Good oral hygiene, Good general health, Permanent dentition, DMF (decay, missing, filling)<5 Exclusion criteria: Use of anti-inflammatory drugs or antibiotics in the last month and during orthodontic treatment, Use of products containing Xylitol, Fluoride, Chlorhexidine, and probiotics or post-biotics

Post-biotic toothpaste group: Participants in this group use toothpaste containing post-biotic twice a day for 4 weeks. Post-biotic mouthwash group: Participants in this group use mouthwash containing post-biotic daily for 4 weeks. Control group: Participants in this group use mouthwash and toothpaste similar to the intervention groups daily for 4 weeks. The only difference between toothpaste and mouthwash used by this group will be the absence of post-biotic.

Number of Streptococcus mutans in saliva before intervention, 4 and 8 weeks after intervention

Block randomization: For this purpose, after creating a computer-generated random sequence using Winpepi software, equal blocks of 6 will be used. unit of randomization is individual and randomization tool is sealed envelopes. These steps are performed by an independent statistician who is also responsible for transferring the envelopes containing the treatment code to the pharmacist in charge of the making of the treatment and placebo products.

When entering the study, each of the participants will be given a number, which is transferred to the pharmacist in charge of the making of the treatment and placebo products. The participants, the person evaluating the results of the intervention and the data analyst will not know the type of treatment of the people. Therapeutic products and placebo are packed in exactly the same opaque sealed bags, there is no difference in terms of color, smell and taste, and the label does not specify the nature of the treatment or placebo.