The Effectiveness of Autologous Platelet-Rich Plasma (PRP) and laser acupuncture in the Therapy of Infertile Men with Non-Obstructive Azoospermia

Survey of effectiveness of autologous platelet-rich plasma and low-power laser in the treatment of male infertility

Eligible men referring to infertility clinics are divided into three groups. All patients are given a consent form.Patients in the first group are injected with PRP intratesticularly and receive laser acupuncture twice a week for 4 consecutive weeks. second group, PRP is injected once, and the third group is considered as a control group without any intervention.To perform PRP, platelet-rich plasma is collected from fresh whole blood from a peripheral vein, stored in acid-citrate dextrose A (ACD-A) anticoagulant solution, and to increase platelets, autologous blood is centrifuged using a kit. and PRP equipment is processed. The intervention is in the form of energy transfer by laser acupuncture and PRP injection on specified points perform. The semen samples taken before the start of the intervention, immediately after the end of the intervention and three months after the end of the intervention.

Shahada Tajrish Hospital and Skin and Stem Cell Research Center

Men under 45 years old with non-obstructive azoospermia, which pathology is confirmed from testicular tissue, and have the history of problems and genetics, drug use during the past year, the presence of varicocele in the examination or ultrasound, the presence of Sertoli cell syndrome in the testicular biopsy report, the absence of testicular descent, the history of acute epidemivaricitis, abnormal examination of the testicles, and also . They were not the risk of having a job with chemical and radioactive materials and obstructive azoospermia.

1- First intervention group: PRP 2- Second intervention group: PRP and laser 3- Control group

sperm motility, concentration and morphology of semen, Sperm count

In this clinical trial study, 45 patients with infertility randomly will be included in the study. For the random allocation of individuals in the study groups, the method of random allocation with block method (Block Randomization) will be used. In this method, blocks with a size of 9 (5 blocks) with a ratio of 1: 1 will be used. Random Allocation software version 2 will be used to generate random sequences. The random allocation concealment method is used in such a way that random sequences are created. In this method, they are identified on the cards and these cards are placed inside the sealed envelopes in order. To maintain the created sequence, the numbering will be done on the outer surface of the envelopes. Finally, the numbered envelopes will be placed in a folder. Then, according to the order of entry of the eligible participants, the envelopes will be opened and the assigned group of the participant will be determined.