The effect of vitamin C supplementation on oxidative stress indices and skin repigmentation of patient with vitiligo

Determining the effect of vitamin C supplementation on serum levels of oxidative stress indicators and skin repigmentation in vitiligo patients

Randomized clinical trial with control group, with parallel groups, double-blind, randomized, phase 2 to 3 on 44 patients, RAS software was used for randomization.

If the patient in the phototherapy clinic of Razi skin hospital in Tehran, has the conditions, first verbal explanations will be given to the patients about the objectives and method of the study. If the patients are willing to cooperate, they will be asked to sign a consent form. Before allocating the study subjects to the intervention and placebo groups, the patients' height, weight, body mass index, and waist circumference will be measured and recorded. Then, an experienced interviewer will collect information related to the individual characteristics of the patients. Then 8 ml of fasting blood samples will be taken from all the subjects for biochemical measurements. Also, vitamin C and placebo tablets in similar packages will be given to patients in two groups in a double-blind manner; And the researcher and the studied people will not know about the type of pills used.

Diagnosis of vitiligo was confirmed by a dermatologist Newly diagnosed vitiligo patient and less than the phototherapy session. Adults 20-60 years old Pregnancy, breastfeeding having history of malignancy, kidney stones, other chronic and inflammatory diseases except vitiligo Taking antibiotics, antivirals and antifungals Use of vitamin C supplements and antioxidants in the last 3 months Any history of alcohol and tobacco use

Vitamin C, Placebo

Total antioxidant capacity Superoxide dismutase Glutathione peroxidase Malondialdehyde Total oxidant status

The study subjects will be block-blocked based on gender and receiving or not receiving phototherapy, and then they will be randomly divided into two intervention groups with vitamin C and placebo. Random allocation: RAS) (size 4) were assigned to intervention and placebo groups, and patients were placed in blocks of 4 based on receiving or not receiving phototherapy and gender. In this study, the participants are randomly placed in two intervention and placebo groups so that researchers can compare different treatments. Researchers and participants cannot arbitrarily play a role in assigning people to groups. Random assignment of people to the intervention or placebo group will be done by an experienced expert.

All subjects and researchers and healthcare personnel who are responsible for the care of patients will be unaware of the existing grouping until the end of the study; In such a way, the researcher and the person who takes the sample, like the study participants, are unaware of which patient will receive the vitamin pill or the placebo. Patients are also unaware of the type of pill received. Drugs and placebos are coded by a person who is completely unaware of the study process, and this code is placed in an envelope that indicates whether it is a drug or a placebo code. One group will receive drug A and one group will receive drug B. Also, pills containing vitamin C and placebo, which have the same color, smell, and size as the vitamin pill, will be given to the study subjects every 4 weeks by another person who has no knowledge of the research process. Hence, this study will be a double-blind study. Placebo tablets contain starch and are completely similar to vitamin C tablets in terms of color, appearance and smell. Placebo tablets are also prepared by the same company that manufactures vitamin C.