Evaluate the effect of platelet-rich plasma(PRP) by testicular injection on spermogram parameters of infertile men with Non-obstructive Azoospermia candidate for TESE

Determining and comparing the effect of platelet-rich plasma by testicular injection on the number, motility, and morphology of sperm in infertile men with non-obstructive ezoospermia candidates for TESE before and after the intervention in two groups

Blocked randomized clinical trial with control group; a blind man; Block randomized; 68 patients.

The research environment is the infertility clinic of Fatemieh Hospital in Hamedan and the time of the research is in 1401-1402. The research population includes all infertile men who are candidates for infertility treatment with non-obstructive azoospermia referring to the infertility clinic of Fatemieh Hospital. The research sample is any infertile man suffering from non-obstructive azoospermia, referring to the infertility clinic of Fatemieh Hospital in Hamadan. For the intervention group, PRP 2CC is injected into the testicle tissue by the same urologist under local anesthesia, and analyzed after three months. Sperm is checked. There is no intervention for the control group.

Entry criteria: age range 20-45 years; lack of sperm in 2 sperm analysis samples; Non-entry criteria: receiving other treatments such as hormone therapy and suffering from underlying diseases (such as diabetes, kidney disease, liver disease, drug and alcohol use, and types of cancer and receiving chemotherapy treatments)

For the intervention group, PRP 2CC is injected into the testicle tissue by the same urologist under local anesthesia, and sperm analysis is checked after three months. There is no intervention for the control group.

Azoospermia

The first stage of selection of those eligible to enter the study is done in an easy and accessible way, but after selecting those eligible to enter the study, the participants are randomly divided into two intervention and control groups. Randomization is done by block method and considering that we have two groups, blocks of 4 are used. Blocked randomization is for the purpose of making sure that exactly the same number of participants enter the 2 control intervention groups in consecutive but equal time intervals. For this purpose, random block is used. 18 sheets of paper are prepared and the letter A is written on 9 sheets and the letter B is written on the other 9 sheets. and upon the application of each eligible person to enter the study, one of the sheets is randomly drawn and assigned to the intervention and control group based on whether it is group A or B, and the drawn sheets until all 18 sheets If it has not been pulled out, it will not be returned to the drawer, and after randomly pulling out all 18 sheets, all the sheets are returned to the drawer and the above procedure is continued on the next 18 patients up to 4 times, i.e. completing the sample volume to 68

Participants are blinded to the treatment method during the study.