After applying the Inclusion and exclusion criteria the method of assigning the subjects will be random allocation by permutation block method. Randomizing is at individual level. The size of the blocks is 4 and in each block, each intervention group will be repeated once. As a result, we will have 24 different blocks, each of which will be numbered from 1 to 24 as desired. Then, using the statistical program code rdunif (n=22, b=24, a=1) in R
software version 3.6.1, 22 blocks of 4 will be generated, which will produce a total of 88 sequences (It should be noted that this code generates 22 random numbers from the numbers 1 to 24, which are the number of blocks). Using this randomly generated list, infants will be placed In the sucrose group, sucrose swaddle, sucrose non-nutritive sucking and sucrose swaddle non nutritive sucking group. To hide the random allocation list, a special code will be assigned to each of the intervention groups that only the executor of plan is aware of. These codes are written on a piece of paper and placed in a sealed envelope. A unique code for each patient will be written on this paper as well as its envelope. A foil is also placed inside each envelope so that the envelopes are not legible under light. Each envelope also contains a white paper and a carbon. All envelopes are randomly placed in a larger box and sealed in the box. The main researcher, after reviewing the inclusion criteria and obtaining informed consent, as well as registering the patient's details in a special form, will contact the partner who has a random assignment list (except for the main researcher who is not involved in the patient recruitment and sample entry process) and randomization of that research sample will be done.
Also, before opening the envelope, this person should write the name and surname and age of the person on the place marked on the envelope so that the writing falls on the paper inside the envelope.