The effect of co-administration of Dietary Approaches To Stop Hypertension (DASH) and Intermittent Fasting (IF) diets on hepatic parameters, glucose homeostasis, lipid profile and inflammatory biomarkers in patients with Non-Alcoholic Fatty Liver Disease (NAFLD): a Randomized Control Trial

Determine the effect of co- administration of DASH and intermittent Fasting (16:8) on liver parameters, glucose homeostasis, blood lipids and inflammatory factors in patients with non-alcoholic fatty liver disease.

Randomized clinical trials with control groups, parallel groups, phase 2 on 50 patients, and random number-producing software will be used for randomization.

50 patients with non-alcoholic fatty liver referred to medical centers who have the conditions to enter the study, they will be included in the study after having a fibroscan test and having a cap score above 260 and willing to participate in the study. At the beginning of the study, an informed consent form is completed for each patient. At the beginning and end of the study (after 12 weeks), fiber scan test, calculation of anthropometric characteristics, completion of physical activity questionnaire, 24-hour recall questionnaire for 3 days, including 2 non-holiday days and 1 holiday day, and taking a blood sample of 5 cc after 12 hours of fasting .

Entry conditions: Age over 18 years, with evidence of non-alcoholic steatohepatitis with CAP Score above 260, without chronic inflammatory disease. conditions of non-entry: pregnancy or use of birth control pills, unwillingness to participate in the study. Weight loss program in the last 3 months. History of weight loss surgery, uncontrolled diabetes, cardiovascular and kidney disease

1- Control(receiving nutritional recommendation to control disease). 2- Intervention:16:8 Intermittent Fasting diet: 16 hours a day in a Intermittent Fasting and get DASH diet in 8 hours of day.

Alanine transaminase, Aspartate aminotransferase, and Gamma-glutamyl transferase ؛Hepatic steatosis and fibrosis؛ Lipid profile and index of insulin resistance and sensitivity

The first step is to create a random sequence number. For this purpose, we will use a random number generator on Stattrek (https://stattrek.com/statistics/random-number-generator.aspx) for 50 numbers ranging from 1 to 2 (for example 2 1 1 2 1 2 1 1 2 2 2 1 1 2 2 1 2 2..............).Then, we will specify the numbers to the groups, for example, 1 will be assigned to the intervention group, and the numbers 2 to the control group. Each number will represent a group according to the defined numbers. With this method, we will have a specific sequence of 50 codes of 1, 2, which shows the first to 50th people who are going to be entered into the study in each group. We write the codes on a piece of paper and put them in an envelope to use during sampling. Thus, after reviewing the inclusion criteria and if the participants desire, we will enter each person included in the study according to our predetermined list.