Protocol summary

Study aim
Determining the effect of 600 mg per day N-acetylcysteine tablet compared to not receiving the drug on the anticoagulant panel with crossover method in non-transfusion dependent thalassemia patients who underwent splenectomy
Design
A controlled, crossover groups, single-blind, randomized, phase 3 clinical trial on 20 patients. Simple randomization will be done using an envelope containing sheets with A and B letters.
Settings and conduct
Patients referring to the Thalassemia Research Center of Bu Ali Hospital who meet the inclusion criteria are divided into two groups of A and B by simple randomization. Group A receive N-acetylcysteine and group B is without medication. After one month, the group A will not receive medication and group B recieves N-acetylcysteine. The only person responsible for conducting the laboratory tests is considered blind. 5 cc of blood samples will be obtained from the patients at the beginning of the study, one month later and at the end of the second month.
Participants/Inclusion and exclusion criteria
All non-transfusion-dependent β-thalassemia patients over 18 years of age who underwent splenectomy and are treated with aspirin will be included in the study
Intervention groups
The patients are randomly divided into two groups: A, who take n-acetylcysteine orally at a dose of 10 mg per body weight (one 600 mg tablet per day) for one month, and B (without medication during the first month). Then, group B will receive the n-acetylcysteine in a cross-over manner in the second month, and group A will be without drug. The washout period is one week.
Main outcome variables
The primary outcome is a reduction of at least 50% in the anticoagulant panel, and the secondary outcomes are a reduction in factor 5 Leiden, protein c and s, antithrombin 3, and lupus anticoagulant.

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT20100110003032N4
Registration date: 2023-10-14, 1402/07/22
Registration timing: registered_while_recruiting

Last update: 2023-10-14, 1402/07/22
Update count: 0
Registration date
2023-10-14, 1402/07/22
Registrant information
Name
Hossein Karami
Name of organization / entity
Mazandaran university of medical sciences
Country
Iran (Islamic Republic of)
Phone
+98 15 1223 4506
Email address
hokarami@mazums.ac.ir
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2023-03-21, 1402/01/01
Expected recruitment end date
2024-03-19, 1402/12/29
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
The effect of N-acetyl cysteine on platelet aggregation in splenectomized non-transfusion dependent thalassemia patients
Public title
The effect of N-acetyl cysteine on platelet aggregation in splenectomized non-transfusion dependent thalassemia patients
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Beta thalassemia Non-transfusion dependent Splenectomy Age above 18 years Under Aspirin therapy
Exclusion criteria:
Age less than 18 years Simultaneous consumption of other antioxidants such as vitamin C and E Diabetes Irregular use of N-acetylcysteine
Age
From 18 years old
Gender
Both
Phase
3
Groups that have been masked
  • Investigator
Sample size
Target sample size: 20
Randomization (investigator's opinion)
Randomized
Randomization description
This study is a randomized clinical trial in which patients are assigned to intervention and control groups by simple randomization method. Using an envelope containing 20 sheets containing 10 A code (intervention) and 10 B code (control), patients are randomly divided into intervention and control groups. The intervention group will receive N-acetylcysteine and the control group will not receive medication. After one month, people in the intervention group will not receive medication and people in the control group will receive N-acetylcysteine.
Blinding (investigator's opinion)
Single blinded
Blinding description
The only person responsible for conducting the lab tests is blind about the use or non-use of the drug by the patients
Placebo
Not used
Assignment
Crossover
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics Commitee of Mazandaran University of Medical Sciences
Street address
Vica chancellor for Research and Technology, Moallem square, Sari
City
Sari
Province
Mazandaran
Postal code
4712855689
Approval date
2022-11-30, 1401/09/09
Ethics committee reference number
IR.MAZUMS.REC.1401.440

Health conditions studied

1

Description of health condition studied
Beta thalassemia
ICD-10 code
D56.1
ICD-10 code description
Beta thalassemia

Primary outcomes

1

Description
Reduction of at least 50% in anticoagulation panel
Timepoint
At the beginning of the study, one month later and the end of the second month
Method of measurement
Laboratory tests including: Factor V Leiden; c and s proteins; antithrombin 3; Lupus Anticoagulant

Secondary outcomes

1

Description
Factor V Leiden
Timepoint
Upon entering the study, 1 month and 2 months later
Method of measurement
Blood test

2

Description
C and S protein
Timepoint
Upon entering the study, 1 month and 2 months later
Method of measurement
Blood test

3

Description
Antithrombin 3
Timepoint
Upon entering the study, 1 month and 2 months later
Method of measurement
Blood test

4

Description
Lupus anticoagulant
Timepoint
Upon entering the study, 1 month and 2 months later
Method of measurement
Blood test

Intervention groups

1

Description
Intervention group: Patients are randomly divided into two groups: A, who recieves n-acetylcysteine orally at a dose of 10 mg per body weight (one 600 mg tablet per day) for one month.
Category
Treatment - Drugs

2

Description
Control group: Group B (control) is without medication during the first month. Then, group B will receive the n-acetylcysteine in a cross-over manner in the second month, and group A will be without drug.
Category
Treatment - Drugs

Recruitment centers

1

Recruitment center
Name of recruitment center
Bu-Ali Sina Hospital
Full name of responsible person
Hossein Karami
Street address
Bu-Ali Sina Hospital, Pasdaran Boulevard, Sari
City
Sari
Province
Mazandaran
Postal code
4815838477
Phone
+98 11 3334 3014
Email
hokarami@mazums.ac.ir

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Mazandaran University of Medical Sciences
Full name of responsible person
Dr. Pedram Ebrahimnejad
Street address
Vice chancellor for Research and Technology, Moallem Square, Sari, Iran
City
Sari
Province
Mazandaran
Postal code
4817844718
Phone
+98 11 3325 7230
Fax
+98 11 3326 1244
Email
p.ebrahimnezhad@mazums.ac.ir
Web page address
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Mazandaran University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Mazandaran University of Medical Sciences
Full name of responsible person
Hossein Karami
Position
Associate professor
Latest degree
Subspecialist
Other areas of specialty/work
Pediatric Hematology and oncologist
Street address
Buali Hospital, Pasdaran Boulevard, Sari
City
Sari
Province
Mazandaran
Postal code
4815838477
Phone
+98 11 3334 3012
Fax
Email
hokarami@mazums.ac.ir

Person responsible for scientific inquiries

Contact
Name of organization / entity
Mazandaran University of Medical Sciences
Full name of responsible person
Dr. Mohaddeseh Momeni
Position
Resident of Pediatrics
Latest degree
Medical doctor
Other areas of specialty/work
Pediatrics
Street address
BuAli Hospital, Pasdaran Boulevard, Sari
City
Sari
Province
Mazandaran
Postal code
4815838477
Phone
+98 11 3334 3012
Email
m.momeni@mazums.ac.ir

Person responsible for updating data

Contact
Name of organization / entity
Mazandaran University of Medical Sciences
Full name of responsible person
Hossein Karami
Position
Associate professor
Latest degree
Subspecialist
Other areas of specialty/work
Pediatric Hematology and Oncologist
Street address
BuAli Hospital, Pasdaran Boulevard, Sari
City
Sari
Province
Mazandaran
Postal code
4817844718
Phone
+98 11 3325 7230
Email
hokarami@mazums.ac.ir
Web page address

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Yes - There is a plan to make this available
Study Protocol
Yes - There is a plan to make this available
Statistical Analysis Plan
No - There is not a plan to make this available
Informed Consent Form
No - There is not a plan to make this available
Clinical Study Report
Yes - There is a plan to make this available
Analytic Code
No - There is not a plan to make this available
Data Dictionary
No - There is not a plan to make this available
Title and more details about the data/document
Part of the data is accessible
When the data will become available and for how long
Starting in January 2025
To whom data/document is available
Everybody
Under which criteria data/document could be used
Systematic review articles
From where data/document is obtainable
Contact Dr. Hossein Karami. E-mail: hokarami@mazums.ac.ir
What processes are involved for a request to access data/document
After contact, information is sent within a few days
Comments
Loading...