View older revisions Content changed at 2025-12-06, 1404/09/15

Protocol summary

Study aim
Evaluation the clinical effects of two regimens with different doses of ampicillin/sulbactam in patients with ventilator associated pneumonia caused by resistant Acinetobacter baumannii
Design
Randomized clinical trial with parallel groups on 60 patients. Block randomization method will be used trough online website.
Settings and conduct
This study is conducted in Loqman Hakim Hospital in Tehran. Patients are divided into two groups after evaluating the inclusion criteria. The first group receives meropenem at a dose of 1 g every 8 hours + colistin at dose of 4.5 million units every 12 hours + ampicillin sulbactam at a dose of 6 g every 6 hours, and the second group receives meropenem at a dose of 1 g every 8 hours + colistin at dose of 4.5 million units every 12 hours + ampicillin sulbactam injection at a dose of 9 grams every 6 hours.
Participants/Inclusion and exclusion criteria
In this study, patients over 18 years are included who their infection caused by a mechanical ventilation device and the resistant Acinetobacter baumannii. The patients sign an informed consent form before entering the study. Breastfeeding or pregnant patients were not included in the study.
Intervention groups
The first group (low dose): receiving Meropenem at a dose of 1 gram every 8 hours + Colistin injection 4.5 million units every 12 hours + Ampicillin/Sulbactam at a dose of 6 grams every 6 hours The second group (high dose): receiving Meropenem at a dose of 1 gram every 8 hours + Colistin 4.5 million units every 12 hours + Ampicillin/Sulbactam at a dose of 9 grams every 6 hours
Main outcome variables
The primary outcome in this study includes the percentage of patients who improved without changing their antibiotic regimen during the study as well as the mortality rate of patients at the end of the study.

General information

Reason for update
The study has been completed and a series of inclusion and exclusion criteria that were required during the study were set.
Acronym
IRCT registration information
IRCT registration number: IRCT20180802040665N2
Registration date: 2024-02-28, 1402/12/09
Registration timing: prospective

Last update: 2025-12-06, 1404/09/15
Update count: 1
Registration date
2024-02-28, 1402/12/09
Registrant information
Name
Ali Saffaei
Name of organization / entity
Country
Iran (Islamic Republic of)
Phone
+98 21 7780 1199
Email address
saffaei@sbmu.ac.ir
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2024-03-05, 1402/12/15
Expected recruitment end date
2024-08-05, 1403/05/15
Actual recruitment start date
2024-03-05, 1402/12/15
Actual recruitment end date
2025-03-21, 1404/01/01
Trial completion date
2025-04-21, 1404/02/01
Scientific title
Clinical Evaluation of Two Regimens with Different Doses of Ampicillin/Sulbactam in Patients with Ventilator Associated Pneumonia Caused By Resistant Acinetobacter Baumannii
Public title
Clinical Evaluation of Ampicillin/Sulbactam in Patients with Ventilator Associated Pneumonia
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Patients aged more than 18 years old VAP diagnosis according to the IDSA guidelines (new pulmonary infiltration with clinical symptoms including fever, purulent secretions, leukocytosis, and decreased blood oxygen levels occurring after 48 hours of mechanical ventilation). Sign the informed consent form Reporting of resistance Acinetobacter in the pulmonary secretion (concurrent resistance to aminoglycosides, fluoroquinolones, cephalosporins, penicillins and carbapenems). Patients who received empiric antibiotic regimen consist of meropenem (at dose of 1000 mg IV q8h) and vancomycin (at dose of 15 mg/kg IV q12h) for the first 48 hours until the results of the culture are determined.
Exclusion criteria:
Breastfeeding and pregnant patients. Patients who have an infection of another source other than the infection caused by the mechanical ventilation device. Obese patients with body mass index more than 35. Patients who have a history of allergy to medicines in the therapeutic regimen. Patients with underlying lung diseases, immunocompromised patients, and those with recurrent lung infection. Patients presenting with sepsis at the commencement of the study, according to the 2021 sepsis campaign guidelines. Individuals with pre-existing pulmonary conditions, such as chronic obstructive pulmonary disease (COPD), lung cancer, or pulmonary thromboembolism (PTE). Patients whose antibiotic treatment may be altered in terms of dosage and administration schedule. Patients experiencing a hypersensitivity reaction during the course of the medication. Patients who have ceased medication for a duration of less than 10 days.
Age
From 18 years old
Gender
Both
Phase
2-3
Groups that have been masked
No information
Sample size
Target sample size: 60
Actual sample size reached: 77
Randomization (investigator's opinion)
Randomized
Randomization description
Block randomization method was used in this study. 15 blocks including 4 patients generated with online website. In each block, two patients will be assigned to group A and two patients will be assigned to group B.
Blinding (investigator's opinion)
Not blinded
Blinding description
Placebo
Not used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Vice-Chancellor in Research Affairs - Shahid Beheshti University of Medical Sciences
Street address
3 rd floor, School of Medicine, Evin St, Shahid Chamran Highway
City
Tehran
Province
Tehran
Postal code
1983963113
Approval date
2023-08-01, 1402/05/10
Ethics committee reference number
IR.SBMU.PHARMACY.REC.1402.094

Health conditions studied

1

Description of health condition studied
Ventilator associated Pneumonia
ICD-10 code
J95.851
ICD-10 code description
Ventilator associated pneumonia

Primary outcomes

1

Description
The percentage of patients who recovered without changing their antibiotic regimen during the study.
Timepoint
10 days after the start of the study
Method of measurement
Relief of fever, reduction of purulent secretions and no need for vasopressor for at least 48 hours

2

Description
Mortality rate
Timepoint
10 days after the start of the study
Method of measurement
Evaluation of patients' medical records

Secondary outcomes

1

Description
The percentage of patients who manage to be weaned from mechanical ventilation or tolerate spontaneous breathing mode.
Timepoint
10 days after the start of the study
Method of measurement
Evaluation of patients' ventilators

2

Description
Length of stay in ICU
Timepoint
10 days after the start of the study
Method of measurement
Evaluation of patients' medical records

3

Description
Percentage of patients who develop the adverse drug reactions
Timepoint
Daily up to 10 days
Method of measurement
Examination of patients

Intervention groups

1

Description
Intervention group: receiving Meropenem injection at a dose of 1 gram every 8 hours + Colistin injection 4.5 million units every 12 hours + Ampicillin/Sulbactam injection at a dose of 9 grams every 6 hours
Category
Treatment - Drugs

2

Description
Control group: receiving Meropenem injection at a dose of 1 gram every 8 hours + Colistin injection 4.5 million units every 12 hours + Ampicillin/Sulbactam injection at a dose of 6 grams every 6 hours
Category
Treatment - Drugs

Recruitment centers

1

Recruitment center
Name of recruitment center
Loghman Hakim hospital
Full name of responsible person
Ilad Alavi Darazam
Street address
Loghman Hakim Hospital, Kamali St, Makhsoos St
City
Tehran
Province
Tehran
Postal code
1333635445
Phone
+98 21 5541 9005
Email
ilad13@yahoo.com

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Shahid Beheshti University of Medical Sciences
Full name of responsible person
Afshin Zarghi
Street address
3 rd floor, School of Medicine, Evin St, Shahid Chamran Highway
City
Tehran
Province
Tehran
Postal code
1983963113
Phone
+98 21 23871
Email
mpd@sbmu.ac.ir
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Shahid Beheshti University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Shahid Beheshti University of Medical Sciences
Full name of responsible person
Zahra Sahraei
Position
Associate professor
Latest degree
Specialist
Other areas of specialty/work
Medical Pharmacy
Street address
Valieasr St, Niyayesh Highway, Shahid Beheshti University of Medical Sciences, School of Pharmacy
City
Tehran
Province
Tehran
Postal code
1991953381
Phone
0098218200118
Email
zahra.sahraei@yahoo.com

Person responsible for scientific inquiries

Contact
Name of organization / entity
Shahid Beheshti University of Medical Sciences
Full name of responsible person
Elmira
Position
Clinical Pharmacy Resident
Latest degree
Medical doctor
Other areas of specialty/work
Medical Pharmacy
Street address
Shool of Pharmacy, Shahid Beheshti University of Medical Sciences, Valiasr St, Tehran
City
Tehran
Province
Tehran
Postal code
1991953381
Phone
0098218200118
Fax
Email
elmira_niknami@yahoo.com

Person responsible for updating data

Contact
Name of organization / entity
Shahid Beheshti University of Medical Sciences
Full name of responsible person
Elmira
Position
Clinical Pharmacy Resident
Latest degree
Medical doctor
Other areas of specialty/work
Medical Pharmacy
Street address
Shool of Pharmacy, Shahid Beheshti University of Medical Sciences, Valiasr St, Tehran
City
Tehran
Province
Tehran
Postal code
1991953381
Phone
0098218200118
Fax
Email
elmira_niknami@yahoo.com

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Yes - There is a plan to make this available
Study Protocol
Undecided - It is not yet known if there will be a plan to make this available
Statistical Analysis Plan
Undecided - It is not yet known if there will be a plan to make this available
Informed Consent Form
Undecided - It is not yet known if there will be a plan to make this available
Clinical Study Report
Undecided - It is not yet known if there will be a plan to make this available
Analytic Code
Undecided - It is not yet known if there will be a plan to make this available
Data Dictionary
Undecided - It is not yet known if there will be a plan to make this available
Title and more details about the data/document
All potential data can be shared after blinding
When the data will become available and for how long
Six mounts after results published
To whom data/document is available
Researchers working in academic institutions
Under which criteria data/document could be used
For research purposes and meta analysis
From where data/document is obtainable
Dr. Zahra Sahraei Valieasr St, Niyayesh Highway, Shahid Beheshti University of Medical Sciences, School of Pharmacy, 3rd floor, Department of Clinical Pharmacy Zip Code: 1996835113 Telephone: +982188200209 Email: z.sahraei@sbmu.ac.ir
What processes are involved for a request to access data/document
Official letter to the researchers, then after 7 days, their request will be answered.
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