Obesity and overweight are increasing because of increased incidence of many diseases such as coronary heart disease. The effect of heat shock proteins in cardiovascular disease has been confirmed. Therefore, simple check-off treatments like acupuncture treatment of obesity while reducing cardiovascular risk factors is important. Participants, male and female aged between 16 to 60, BMI>25, without drug history and Heart disease, (n=550), were randomized to therapeutic body acupuncture, auricular acupuncture, sham groups (sham body and auricular acupuncture) and control group just with low calorie diet. Subjects received authentic (cases) or sham acupuncture and control for 6-weeks (first-period) in combination with a low-calorie diet. In the next 6-week (second-period), the low-calorie diet was used on its own. Subjects were assessed at the beginning, 6 and 12 weeks later. In addition to anthropometric and lipid parameters, serum anti-heat shock protein (Hsp)-27, 60, 65, 70 and high sensitive C-reactive protein (hs-CRP) levels were assessed.